LOCUS       sequence-42335-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PP-MAPK2 (3RP9)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccctctgaat attttgttta ctttaagaag gagatatacc atgcatcatc atcatcatca
       61 cagcagcggc agagaaaact tgtatttcca gggccatcac agctctaaac ctactgctag
      121 tatgccgcgc ccgcattcag attggcaaat tccagaccgt tatgagatcc gccacctgat
      181 tgggacagga tcgtacggcc atgtatgcga agcctatgat aaactggaaa aacgtgtggt
      241 tgcaatcaaa aaaattctgc gcgtctttga ggacctgatc gattgtaaac gtattctgcg
      301 cgaaatcgcg attctgaatc gtctgaacca tgatcacgtg gtaaaagttc tggacatcgt
      361 gattcctaaa gatgtcgaga aattcgacga actgtatgtt gtactggaaa tcgctgattc
      421 ggactttaaa aaactgttcc gcaccccggt gtacctgacg gagctgcata ttaaaaccct
      481 gctgtataat ctgctggtcg gtgttaaata cgtgcacagc gccggcatcc tgcatcgtga
      541 tctgaaaccg gcaaactgcc tggtaaatca ggattgttct gtcaaagttt gcgactttgg
      601 tctggcgcgc actgtggatt atccagaaaa cgggaatagt caactgccta tttcaccgcg
      661 tgaggacgat atgaacctgg taacattccc acacacgaaa aatctgaaac gccagctgac
      721 cggacatgtt gtgactcgtt ggtaccgcgc tccggaactg atcctgctgc aggaaaacta
      781 tacagaggcc attgatgtct ggtccattgg ctgtatcttt gcagaactgc tga
//
