LOCUS       sequence-423362-        8251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human CTNNB1. N-terminal NanoLuc
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-N_CTNNB1:1-781
FEATURES             Location/Qualifiers
     source          1..8251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1065..1577
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1608..3953
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     primer_bind     complement(4019..4037)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4069..4190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4080..4099)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4134..4153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4435..4792
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4435..4626
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4458..4478)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4643..4778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4823..5860
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             5839..5847
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5884..5932
                     /note="synthetic polyadenylation signal"
     promoter        6088..6192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6193..6261,6262..7053)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6411..6430)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7211..7799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7291..7310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    7915..8198
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgcttc cgaattcatg gctactcaag
     1621 ctgatttgat ggagttggac atggccatgg aaccagacag aaaagcggct gttagtcact
     1681 ggcagcaaca gtcttacctg gactctggaa tccattctgg tgccactacc acagctcctt
     1741 ctctgagtgg taaaggcaat cctgaggaag aggatgtgga tacctcccaa gtcctgtatg
     1801 agtgggaaca gggattttct cagtccttca ctcaagaaca agtagctgat attgatggac
     1861 agtatgcaat gactcgagct cagagggtac gagctgctat gttccctgag acattagatg
     1921 agggcatgca gatcccatct acacagtttg atgctgctca tcccactaat gtccagcgtt
     1981 tggctgaacc atcacagatg ctgaaacatg cagttgtaaa cttgattaac tatcaagatg
     2041 atgcagaact tgccacacgt gcaatccctg aactgacaaa actgctaaat gacgaggacc
     2101 aggtggtggt taataaggct gcagttatgg tccatcagct ttctaaaaag gaagcttcca
     2161 gacacgctat catgcgttct cctcagatgg tgtctgctat tgtacgtacc atgcagaata
     2221 caaatgatgt agaaacagct cgttgtaccg ctgggacctt gcataacctt tcccatcatc
     2281 gtgagggctt actggccatc tttaagtctg gaggcattcc tgccctggtg aaaatgcttg
     2341 gttcaccagt ggattctgtg ttgttttatg ccattacaac tctccacaac cttttattac
     2401 atcaagaagg agctaaaatg gcagtgcgtt tagctggtgg gctgcagaaa atggttgcct
     2461 tgctcaacaa aacaaatgtt aaattcttgg ctattacgac agactgcctt caaattttag
     2521 cttatggcaa ccaagaaagc aagctcatca tactggctag tggtggaccc caagctttag
     2581 taaatataat gaggacctat acttacgaaa aactactgtg gaccacaagc agagtgctga
     2641 aggtgctatc tgtctgctct agtaataagc cggctattgt agaagctggt ggaatgcaag
     2701 ctttaggact tcacctgaca gatccaagtc aacgtcttgt tcagaactgt ctttggactc
     2761 tcaggaatct ttcagatgct gcaactaaac aggaagggat ggaaggtctc cttgggactc
     2821 ttgttcagct tctgggttca gatgatataa atgtggtcac ctgtgcagct ggaattcttt
     2881 ctaacctcac ttgcaataat tataagaaca agatgatggt ctgccaagtg ggtggtatag
     2941 aggctcttgt gcgtactgtc cttcgggctg gtgacaggga agacatcact gagcctgcca
     3001 tctgtgctct tcgtcatctg accagccgac accaagaagc agagatggcc cagaatgcag
     3061 ttcgccttca ctatggacta ccagttgtgg ttaagctctt acacccacca tcccactggc
     3121 ctctgataaa ggctactgtt ggattgattc gaaatcttgc cctttgtccc gcaaatcatg
     3181 cacctttgcg tgagcagggt gccattccac gactagttca gttgcttgtt cgtgcacatc
     3241 aggataccca gcgccgtacg tccatgggtg ggacacagca gcaatttgtg gagggggtcc
     3301 gcatggaaga aatagttgaa ggttgtaccg gagcccttca catcctagct cgggatgttc
     3361 acaaccgaat tgttatcaga ggactaaata ccattccatt gtttgtgcag ctgctttatt
     3421 ctcccattga aaacatccaa agagtagctg caggggtcct ctgtgaactt gctcaggaca
     3481 aggaagctgc agaagctatt gaagctgagg gagccacagc tcctctgaca gagttacttc
     3541 actctaggaa tgaaggtgtg gcgacatatg cagctgctgt tttgttccga atgtctgagg
     3601 acaagccaca agattacaag aaacggcttt cagttgagct gaccagctct ctcttcagaa
     3661 cagagccaat ggcttggaat gagactgctg atcttggact tgatattggt gcccagggag
     3721 aaccccttgg atatcgccag gatgatccta gctatcgttc ttttcactct ggtggatatg
     3781 gccaggatgc cttgggtatg gaccccatga tggaacatga gatgggtggc caccaccctg
     3841 gtgctgacta tccagttgat gggctgccag atctggggca tgcccaggac ctcatggatg
     3901 ggctgcctcc aggtgacagc aatcagctgg cctggtttga tactgacctg taagatatct
     3961 agatttgggc ccaattcctg caggattttg cggccgcttg ctgagttggc tgctgccacc
     4021 gctgagcaat aactagcata accccttggc cgcttcgagc agacatgata agatacattg
     4081 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4141 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4201 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt
     4261 aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa tattaacgct tacaatttcc
     4321 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg
     4381 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     4441 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4501 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4561 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4621 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4681 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4741 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcga
     4801 ttcttctgac actagcgcca ccatgaagaa gcccgaactc accgctacca gcgttgaaaa
     4861 atttctcatc gagaagttcg acagtgtgag cgacctgatg cagttgtcgg agggcgaaga
     4921 gagccgagcc ttcagcttcg atgtcggcgg acgcggctat gtactgcggg tgaatagctg
     4981 cgctgatggc ttctacaaag accgctacgt gtaccgccac ttcgccagcg ctgcactacc
     5041 catccccgaa gtgttggaca tcggcgagtt cagcgagagc ctgacatact gcatcagtag
     5101 acgcgcccaa ggcgttactc tccaagacct ccccgaaaca gagctgcctg ctgtgttaca
     5161 gcctgtcgcc gaagctatgg atgctattgc cgccgccgac ctcagtcaaa ccagcggctt
     5221 cggcccattc gggccccaag gcatcggcca gtacacaacc tggcgggatt tcatttgcgc
     5281 cattgctgat ccccatgtct accactggca gaccgtgatg gacgacaccg tgtccgccag
     5341 cgtagctcaa gccctggacg aactgatgct gtgggccgaa gactgtcccg aggtgcgcca
     5401 cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc gacaacggcc gcatcaccgc
     5461 cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag tacgaggtgg ccaacatctt
     5521 cttctggcgg ccctggctgg cttgcatgga gcagcagact cgctacttcg agcgccggca
     5581 tcccgagctg gccggcagcc ctcgtctgcg agcctacatg ctgcgcatcg gcctggatca
     5641 gctctaccag agcctcgtgg acggcaactt cgacgatgct gcctgggctc aaggccgctg
     5701 cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc acacaaatcg ctcgccggag
     5761 cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc gacagcggca accgccggcc
     5821 cagtacacga ccgcgcgcta aggaggtagg tcgagtttag actctagcca tcacgatggc
     5881 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     5941 gcgataagga tcctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     6001 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtaatggt ttcttagacg
     6061 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6181 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6241 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6301 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6361 agttttcgcc ccgaagaacg ttttccaatg atgagcactt tcaaagttct gctatgtggc
     6421 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6481 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6541 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6601 ctgacaacta tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6661 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6721 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6781 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6841 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6901 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6961 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7021 gagataggtg cctcactgat taagcattgg taattcgaaa tgaccgacca agcgacgccc
     7081 aaccggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7141 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7201 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7261 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7321 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7381 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7441 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7501 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7561 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7621 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7681 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7741 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag
     7801 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7861 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa
     7921 atacaggaac gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag
     7981 cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata
     8041 cggttaaaat ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta
     8101 cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta
     8161 tggtaaatcc acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc
     8221 acgatgcgtc cggcgtagag gatcgagatc t
//
