LOCUS       sequence-423367-        6525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR91. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_WDR91:1-747
FEATURES             Location/Qualifiers
     source          1..6525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3588..3709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3599..3618)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3824..3857)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3867..3914
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3946..4740
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4000..4019)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4610..4629
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4898..5486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4978..4997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5612..5660
                     /note="synthetic polyadenylation signal"
     CDS             complement(5684..6082)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6079..6088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6113..6470)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6127..6262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6279..6470
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6427..6447
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggcggaggc
     1261 cgtggagcgc actgacgagc tggtccggga gtacctgctc ttccgcgggt tcacgcacac
     1321 actgcggcag ctggacgccg agatcaaggc ggacaaggag aaggggttcc gggtggataa
     1381 gattgtggac cagctgcagc agttaatgca ggtgtatgac ttggctgccc ttcgggatta
     1441 ttggagctac ttggagcgtc ggctcttcag ccgcttggag gatatataca gacccacaat
     1501 ccacaagctg aaaaccagcc tgtttcgatt ttatcttgtc tacacaatcc agacaaacag
     1561 aaatgacaag gctcaggagt tctttgcaaa gcaggccacg gaactccaga accaggctga
     1621 gtggaaggat tggtttgtcc tgcccttcct gccatccccg gacaccaacc ccacctttgc
     1681 tacctacttt tctcgacagt gggctgacac cttcattgtg tccctgcaca acttcctgag
     1741 cgtcctgttt cagtgcatgc cagtccctgt gatcctgaac tttgatgcgg agtgtcagag
     1801 gactaaccag gttcaagaag aaaatgaagt tctgcgtcag aagctttttg cattgcaagc
     1861 tgaaatccac cgactgaaga aagaggagca acagccagaa gaggaagagg ccttggtcca
     1921 acacaaattg cctccttatg tctccaacat ggaccgcctg ggggactcgg aacttgccat
     1981 ggtgtgcagc caaaggaatg cctccctctc ccagtcacct cgtgtgggct tcctgtcctc
     2041 gctgctgcct cagagtaaga agagcccctc aaggttgtcg cctgctcagg gccctcctca
     2101 acctcagagc tcggccaaga aagagtcctt cggtggtcag ggcaccaagg gaaaggaccc
     2161 gacgtccgga gccaaggatg ggaagagcct cctcagcggg ctggccactg gggagtccgg
     2221 ttggtcacag caccggcagc ggcgcctgca ggaccatggc aaggagagga aggagctttt
     2281 ctccacaacc acttcccagt gtgcagagaa gaaaccagaa gccagtggcc cagaggctga
     2341 gccctgccca gagctccaca cggagccagt ggagccactg actcgggcat cctcggcagg
     2401 ccctgagggt ggaggagtcc gccccgagca gccctttatt gtgctgggac aggaggagta
     2461 cggggaacac cactcatcca tcatgcactg cagagtggac tgctctggga ggagagtcgc
     2521 cagcttagac gtagatgggg tcatcaaagt gtggtccttc aaccccatca tgcagaccaa
     2581 agcatcctcc atttccaaat caccgctgct gtctttggaa tgggccacca aacgggacag
     2641 actgctcttg ctgggcagtg gtgtgggaac agtgcgtctc tatgacacgg aagccaagaa
     2701 gaatctctgt gaaatcaata tcaacgacaa catgcccaga atcctgtctc ttgcgtgcag
     2761 ccccaacggg gcctctttcg tctgttcggc agcagctccg agcctcactt cccaggtgga
     2821 cttctcagca ccagacatcg gcagcaaggg catgaaccag gttcctggca ggctgctgct
     2881 gtgggacacg aaaaccatga agcagcagct ccagttctcc ctggatccag aacccattgc
     2941 tatcaactgt acagccttca atcacaacgg gaacctgctg gtcacagggg cagctgatgg
     3001 cgtcatccgg ctgtttgaca tgcagcagca tgagtgcgcg atgagctgga gggcccacta
     3061 cggggaggtc tactctgtgg agttcagcta tgatgagaac accgtgtaca gcatcggcga
     3121 ggacgggaag ttcatccagt ggaacatcca caagagtggc ctcaaggtat ccgagtacag
     3181 cctcccctca gatgccacgg gcccctttgt gctgtctgga tacagcggct acaagcaggt
     3241 tcaagtcccc aggggccgac tcttcgcttt tgactcggag ggaaattaca tgctgacatg
     3301 ttctgccaca ggcggcgtca tctacaagct gggtggcgat gagaaggttc tggagagctg
     3361 cttgagccta ggtggccacc gagcccctgt ggtcaccgtg gactggagca ctgccatgga
     3421 ctgtgggacc tgcctcaccg cctccatgga tggcaagatc aagctgacca ccctcctggc
     3481 ccataaagcc taatctagag gtcaagcttc gggggtttaa acctgcaggc atgcaagctg
     3541 atttggctgc tgccaccgct gagcaaggcc gcttcgagca gacatgataa gatacattga
     3601 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3661 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3721 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
     3781 aaacctctac aaatgtggta aaatcttaat taagacttct aatataactt cgtatagcat
     3841 acattatacg aagttatggt tccactgttg acaattaatc atcggcatag tatatcggca
     3901 tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat
     3961 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4021 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4081 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4141 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4201 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4261 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4321 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4381 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4441 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4501 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4561 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4621 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4681 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4741 ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag gcggtaatac
     4801 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4861 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4921 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4981 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5041 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5101 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5161 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5221 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5281 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5341 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5401 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5461 ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg
     5521 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5581 tcacctagat ccttttatag tccggaatcg tcacacaaaa aaccaacaca cagatgtaat
     5641 gaaaataaag atattttatt gcggccatcg tgatggctag agtttagccc tcccacacat
     5701 aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca
     5761 ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc
     5821 tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg
     5881 cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata
     5941 cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt
     6001 cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt
     6061 cttgagacaa aggcttggcc atggtggcgc tagtgtcaga agaatcgagc tttttgcaaa
     6121 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     6181 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     6241 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     6301 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     6361 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     6421 ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag aaggtaccta
     6481 accaagttcc tctttcagag gttatttcag gccatggtgc tgcgc
//
