LOCUS       sequence-423372-        8136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human MTA2. N-terminal HaloTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pHTN-HaloTag_MTA2:1-668
FEATURES             Location/Qualifiers
     source          1..8136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1067..1957
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1970..1990
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     primer_bind     complement(4087..4105)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4137..4258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4148..4167)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4202..4221
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4503..4860
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4503..4694
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4526..4546)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4711..4846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4911..5705
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4965..4984)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5575..5594
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5769..5817
                     /note="synthetic polyadenylation signal"
     promoter        5973..6077
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6078..6146,6147..6938)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6296..6315)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7096..7684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7176..7195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    7800..8083
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgcttccga attcatggcg gccaacatgt accgggtggg
     2041 agattacgtc tattttgaga actcttccag caatccttac ctggttagac ggattgagga
     2101 gctcaacaag actgcaaatg gaaatgtgga ggcaaaggtt gtctgtcttt tccggcgcag
     2161 ggacatttct agtagcctca acagcctggc tgatagtaat gccagggagt ttgaagagga
     2221 atcaaagcag ccaggggtgt ctgagcagca gcgccatcaa ctgaagcacc gggaactttt
     2281 tctttctcgg caatttgaat cattaccagc cacccacata cgggggaaat gcagtgtgac
     2341 cctcttgaat gagacagata tcttgagcca gtacctggaa aaggaggact gcttttttta
     2401 ctcactggtg tttgaccccg tgcagaagac acttctcgct gatcagggcg agattagagt
     2461 tggttgcaaa taccaagctg agatcccaga tcgcctagta gagggagaat ctgataatcg
     2521 gaaccagcag aagatggaga tgaaggtctg ggacccagac aaccctctca cagaccggca
     2581 gatcgaccag tttcttgtgg tggcccgagc tgtgggaacc tttgcaagag ccctagattg
     2641 tagcagctcc attcggcagc caagcttgca catgagtgca gctgctgcct cccgagatat
     2701 cactctgttt cacgccatgg ataccttgca aaggaacggc tacgacctgg ctaaggccat
     2761 gtcgaccctg gtaccccagg gaggcccggt gctgtgtcgg gatgagatgg aggaatggtc
     2821 agcctcagag gccatgctat ttgaggaggc cctagagaag tatgggaagg acttcaatga
     2881 tattcgccag gattttctac cctggaagtc acttgccagc atagtccagt tttattacat
     2941 gtggaaaacc acagaccggt atattcagca gaaaaggttg aaagctgctg aagcagacag
     3001 caaactgaaa caggtctaca ttcccaccta cactaagcca aaccctaacc agatcatttc
     3061 tgtgggttca aaacctggca tgaatggggc tggatttcag aagggcctga cttgtgagag
     3121 ttgccacacc acacagtctg ctcagtggta tgcctggggc ccacctaaca tgcagtgccg
     3181 cctctgtgct tcctgttgga tctactggaa gaagtatggg ggactgaaga ccccaactca
     3241 gcttgagggg gccactcggg gcaccacgga gccacactca aggggtcatt tatccagacc
     3301 tgaagctcaa agtctctctc cttacacaac cagcgccaac agggccaagc tactggctaa
     3361 gaacagacaa actttcctgc ttcagaccac aaagctgacc cgtcttgcca gacgcatgtg
     3421 cagggaccta ttacagccaa ggagggccgc ccgacggcct tatgctccta tcaatgccaa
     3481 tgccatcaaa gcagagtgct ccattcgact tcctaaggcc gccaagactc cattgaagat
     3541 tcaccctctg gtgcggctgc ccctggcaac tatcgtcaaa gatctggtgg cccaggcacc
     3601 cctgaaacca aaaacacctc ggggtaccaa gacaccgatc aacagaaacc agctgtccca
     3661 gaaccgggga ctggggggca ttatggtgaa acgggcctat gagactatgg caggggcagg
     3721 ggttcctttc tctgccaatg gaaggcctct ggcttcaggg attcgttcaa gctcacagcc
     3781 agcagccaag cgtcagaaac taaacccagc tgatgccccc aatcctgtgg tgtttgtggc
     3841 cacaaaggat accagggccc tacggaaggc tctgacccat ctggaaatgc ggcgagctgc
     3901 tcgccgaccc aacttgcccc tgaaggtgaa gccaacgctg attgcagtgc ggccccctgt
     3961 ccctctacct gcaccctcac atcctgccag caccaatgag cctattgtcc tggaggactg
     4021 agatatctag atttgggccc aattcctgca ggattttgcg gccgcttgct gagttggctg
     4081 ctgccaccgc tgagcaataa ctagcataac cccttggccg cttcgagcag acatgataag
     4141 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4201 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4261 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     4321 aagcaagtaa aacctctaca aatgtggtaa aatcgaattt taacaaaata ttaacgctta
     4381 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4441 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     4501 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4621 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4681 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4741 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4801 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4861 aagcttgatt cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac
     4921 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4981 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5041 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5101 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5161 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5221 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5281 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5341 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5401 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5461 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5521 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5581 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5641 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5701 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5761 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     5821 cgatagcgat aaggatcctc tttgcgcttg cgttttccct tgtccagata gcccagtagc
     5881 tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgta atggtttctt
     5941 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6001 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6061 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6121 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6181 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6241 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cactttcaaa gttctgctat
     6301 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6361 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6421 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6481 tacttctgac aactatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6541 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6601 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6661 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6721 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6781 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6841 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6901 tcgctgagat aggtgcctca ctgattaagc attggtaatt cgaaatgacc gaccaagcga
     6961 cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7081 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7501 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt
     7681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7741 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttatagtc
     7801 cggaaataca ggaacgcacg ctggatggcc cttcgctggg atggtgaaac catgaaaaat
     7861 ggcagcttca gtggattaag tgggggtaat gtggcctgta ccctctggtt gcataggtat
     7921 tcatacggtt aaaatttatc aggcgcgatt gcggcagttt ttcgggtggt ttgttgccat
     7981 ttttacctgt ctgctgccgt gatcgcgctg aacgcgtttt agcggtgcgt acaattaagg
     8041 gattatggta aatccactta ctgtctgccc tcgtagccat cgagataaac cgcagtactc
     8101 cggccacgat gcgtccggcg tagaggatcg agatct
//
