LOCUS       sequence-423379-        6569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR91. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_WDR91:1-747
FEATURES             Location/Qualifiers
     source          1..6569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3632..3753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3643..3662)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3868..3901)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3911..3958
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3990..4784
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4044..4063)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4654..4673
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4942..5530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5022..5041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5656..5704
                     /note="synthetic polyadenylation signal"
     CDS             complement(5728..6126)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6123..6132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6157..6514)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6171..6306
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6323..6514
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6471..6491
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggcgga ggccgtggag
     1261 cgcactgacg agctggtccg ggagtacctg ctcttccgcg ggttcacgca cacactgcgg
     1321 cagctggacg ccgagatcaa ggcggacaag gagaaggggt tccgggtgga taagattgtg
     1381 gaccagctgc agcagttaat gcaggtgtat gacttggctg cccttcggga ttattggagc
     1441 tacttggagc gtcggctctt cagccgcttg gaggatatat acagacccac aatccacaag
     1501 ctgaaaacca gcctgtttcg attttatctt gtctacacaa tccagacaaa cagaaatgac
     1561 aaggctcagg agttctttgc aaagcaggcc acggaactcc agaaccaggc tgagtggaag
     1621 gattggtttg tcctgccctt cctgccatcc ccggacacca accccacctt tgctacctac
     1681 ttttctcgac agtgggctga caccttcatt gtgtccctgc acaacttcct gagcgtcctg
     1741 tttcagtgca tgccagtccc tgtgatcctg aactttgatg cggagtgtca gaggactaac
     1801 caggttcaag aagaaaatga agttctgcgt cagaagcttt ttgcattgca agctgaaatc
     1861 caccgactga agaaagagga gcaacagcca gaagaggaag aggccttggt ccaacacaaa
     1921 ttgcctcctt atgtctccaa catggaccgc ctgggggact cggaacttgc catggtgtgc
     1981 agccaaagga atgcctccct ctcccagtca cctcgtgtgg gcttcctgtc ctcgctgctg
     2041 cctcagagta agaagagccc ctcaaggttg tcgcctgctc agggccctcc tcaacctcag
     2101 agctcggcca agaaagagtc cttcggtggt cagggcacca agggaaagga cccgacgtcc
     2161 ggagccaagg atgggaagag cctcctcagc gggctggcca ctggggagtc cggttggtca
     2221 cagcaccggc agcggcgcct gcaggaccat ggcaaggaga ggaaggagct tttctccaca
     2281 accacttccc agtgtgcaga gaagaaacca gaagccagtg gcccagaggc tgagccctgc
     2341 ccagagctcc acacggagcc agtggagcca ctgactcggg catcctcggc aggccctgag
     2401 ggtggaggag tccgccccga gcagcccttt attgtgctgg gacaggagga gtacggggaa
     2461 caccactcat ccatcatgca ctgcagagtg gactgctctg ggaggagagt cgccagctta
     2521 gacgtagatg gggtcatcaa agtgtggtcc ttcaacccca tcatgcagac caaagcatcc
     2581 tccatttcca aatcaccgct gctgtctttg gaatgggcca ccaaacggga cagactgctc
     2641 ttgctgggca gtggtgtggg aacagtgcgt ctctatgaca cggaagccaa gaagaatctc
     2701 tgtgaaatca atatcaacga caacatgccc agaatcctgt ctcttgcgtg cagccccaac
     2761 ggggcctctt tcgtctgttc ggcagcagct ccgagcctca cttcccaggt ggacttctca
     2821 gcaccagaca tcggcagcaa gggcatgaac caggttcctg gcaggctgct gctgtgggac
     2881 acgaaaacca tgaagcagca gctccagttc tccctggatc cagaacccat tgctatcaac
     2941 tgtacagcct tcaatcacaa cgggaacctg ctggtcacag gggcagctga tggcgtcatc
     3001 cggctgtttg acatgcagca gcatgagtgc gcgatgagct ggagggccca ctacggggag
     3061 gtctactctg tggagttcag ctatgatgag aacaccgtgt acagcatcgg cgaggacggg
     3121 aagttcatcc agtggaacat ccacaagagt ggcctcaagg tatccgagta cagcctcccc
     3181 tcagatgcca cgggcccctt tgtgctgtct ggatacagcg gctacaagca ggttcaagtc
     3241 cccaggggcc gactcttcgc ttttgactcg gagggaaatt acatgctgac atgttctgcc
     3301 acaggcggcg tcatctacaa gctgggtggc gatgagaagg ttctggagag ctgcttgagc
     3361 ctaggtggcc accgagcccc tgtggtcacc gtggactgga gcactgccat ggactgtggg
     3421 acctgcctca ccgcctccat ggatggcaag atcaagctga ccaccctcct ggcccataaa
     3481 gccggctcga gcggtgggag ctccggtgtg agcggctggc ggctgttcaa gaagattagc
     3541 taagtttaaa cggccgcgac tctagactgc aggcatgcaa gctgatttgg ctgctgccac
     3601 cgctgagcaa ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     3661 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     3721 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3781 tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt
     3841 ggtaaaatct taattaagac ttctaatata acttcgtata gcatacatta tacgaagtta
     3901 tggttccact gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     3961 ggtgaggaac taaacccagg aggcagatca tgattgaaca agatggattg cacgcaggtt
     4021 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4081 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4141 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     4201 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4261 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4321 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4381 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4441 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4501 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4561 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4621 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4681 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4741 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgattcgaa atgaccgacc
     4801 aagcgacgcc caaccggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4861 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5341 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     5401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5521 aggatttcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5641 atagtccgga atcgtcacac aaaaaaccaa cacacagatg taatgaaaat aaagatattt
     5701 tattgcggcc atcgtgatgg ctagagttta gccctcccac acataaccag agggcagcaa
     5761 ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc ttcactatgg ctttgatccc
     5821 aggatgcaga tcgagaagca cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt
     5881 ctcatttccg atcgcgacga tacaagtcag gttgccagct gccgcagcag cagcagtgcc
     5941 cagcaccacg agttctgcac aaggtccccc agtaaaatga tatacattga caccagtgaa
     6001 gatgcggccg tcgctagaga gagctgcgct ggcgacgctg tagtcttcag agatggggat
     6061 gctgttgatt gtagccgttg ctctttcaat gagggtggat tcttcttgag acaaaggctt
     6121 ggccatggtg gcgctagtgt cagaagaatc gagctttttg caaaagccta ggcctccaaa
     6181 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     6241 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     6301 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     6361 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     6421 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     6481 acacacattc cacagctggt tctttccgcc tcagaaggta cctaaccaag ttcctctttc
     6541 agaggttatt tcaggccatg gtgctgcgc
//
