LOCUS       sequence-423413-        6806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human ELP2. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_ELP2:1-826
FEATURES             Location/Qualifiers
     source          1..6806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1241..1250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3869..3990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3880..3899)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4105..4138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4148..4195
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4227..5021
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4281..4300)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4891..4910
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5179..5767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5259..5278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5893..5941
                     /note="synthetic polyadenylation signal"
     CDS             complement(5965..6363)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6360..6369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6394..6751)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6408..6543
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6560..6751
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6708..6728
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggtggc acccgtgctg
     1261 gagacttctc acgtgttttg ctgcccaaac cgggtgcggg gagtcctgaa ctggagctct
     1321 gggcccagag gacttctggc ctttggcacg tcctgctccg tggtgctcta tgaccccctg
     1381 aaaagggttg ttgttaccaa cttgaatggt cacaccgccc gagtcaattg catacagtgg
     1441 atttgtaaac aggatggctc cccttctact gaattagttt ctggaggatc tgataatcaa
     1501 gtgattcact gggaaataga ggataatcag cttttaaaag cagtgcatct tcaaggccat
     1561 gaaggacctg tttatgcggt gcatgctgtt taccagagga ggacatcaga tcctgcatta
     1621 tgtccactga tcgtttctgc agctgcagat tctgctgttc gactctggtc taaaaagggt
     1681 ccagaagtaa tgtgccttca gactttaaac tttggaaatg gatttgcttt ggctctctgc
     1741 ttatcttttt tgccaaatac tgatgtacca atattagcat gtggcaatga tgattgcaga
     1801 attcacatat ttgctcaaca aaatgatcag tttcagaaag tgctttctct ctgtggacat
     1861 gaggattgga ttagaggagt ggaatgggca gcctttggta gagatctttt cctagcaagc
     1921 tgttcacaag attgcctgat aagaatatgg aagctgtata taaagtcaac atctttagaa
     1981 actcaggatg acgataacat aagactgaaa gaaaatactt ttaccataga aaatgaaagt
     2041 gttaaaatag catttgctgt tactctggag acagtgctag ccggtcatga aaactgggta
     2101 aatgcagttc actggcaacc tgtgttttac aaagatggtg tcctacagca gccaatgaga
     2161 ttattatctg cttccatgga taaaaccatg attctctggg ctccagatga agagtcagga
     2221 gtttggctag aacaggttcg agtaggtgaa gtaggtggga atactttggg attttatgat
     2281 tgccagttca atgaagatgg ctccatgatc attgctcatg ctttccacgg agcgttgcac
     2341 ctttggaaac agaatacagt taacccaaga gagtggactc cagagattgt catttcagga
     2401 cactttgatg gtgtccaaga cctagtctgg gatccagaag gagaatttat tatcactgtt
     2461 ggtactgatc agacaactag actttttgct ccatggaaga gaaaagacca atcacaggtg
     2521 acttggcatg aaattgcaag gcctcagata catgggtatg acctgaaatg tttggcaatg
     2581 attaatcggt ttcagtttgt atctggagca gatgaaaaag ttcttcgggt tttttctgca
     2641 cctcggaatt ttgtggaaaa tttttgtgcc attacaggac aatcactgaa tcatgtgctc
     2701 tgtaatcaag atagtgatct tccagaagga gccactgtcc ctgcattggg attatcaaat
     2761 aaagctgtct ttcagggaga tatagcttct cagccttctg atgaagagga gctgttaact
     2821 agtactggtt ttgagtatca gcaggtggcc tttcagccct ccatacttac tgagcctccc
     2881 actgaggatc atcttctgca gaatactttg tggcctgaag ttcaaaaact atatgggcac
     2941 ggttatgaaa tattttgtgt tacttgtaac agttcaaaga ctctgcttgc ctcagcttgt
     3001 aaggcagcta agaaagagca tgcagctatc attctttgga acactacatc ttggaaacag
     3061 gtgcagaatt tagttttcca cagtttgaca gtcacgcaga tggccttctc acctaatgag
     3121 aagttcttac tagctgtttc cagagatcga acctggtcat tgtggaaaaa gcaggataca
     3181 atctcacctg agttcgagcc agtttttagt ctttttgcct tcaccaacaa aattacttct
     3241 gtgcacagta gaattatttg gtcttgtgat tggagtcctg acagcaagta tttcttcact
     3301 gggagtcgag acaaaaaggt ggttgtctgg ggtgagtgtg actccactga tgactgtatt
     3361 gagcacaaca ttggcccctg ctcctcagtc ctggacgtgg gtggggctgt gacagctgtc
     3421 agcgtctgcc cagtgctcca cccttctcaa cgatacgtgg ttgcagtagg attggagtgt
     3481 ggaaagattt gcttatatac ctggaaaaag actgatcaag ttccagaaat aaatgactgg
     3541 acccactgtg tagaaacaag tcaaagccaa agtcatacac tggctatcag aaaattatgc
     3601 tggaagaatt gcagtggaaa aactgaacag aaggaagcag aaggtgctga gtggttacac
     3661 tttgcaagct gtggtgaaga tcacactgtg aagatacaca gagtcaataa atgtgcactg
     3721 ggctcgagcg gtgggagctc cggtgtgagc ggctggcggc tgttcaagaa gattagctaa
     3781 gtttaaacgg ccgcgactct agactgcagg catgcaagct gatttggctg ctgccaccgc
     3841 tgagcaaggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     3901 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3961 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4021 caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt
     4081 aaaatcttaa ttaagacttc taatataact tcgtatagca tacattatac gaagttatgg
     4141 ttccactgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     4201 gaggaactaa acccaggagg cagatcatga ttgaacaaga tggattgcac gcaggttctc
     4261 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4321 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4381 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     4441 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4501 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     4561 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     4621 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     4681 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     4741 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     4801 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4861 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4921 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     4981 agcgcatcgc cttctatcgc cttcttgacg agttcttctg attcgaaatg accgaccaag
     5041 cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5581 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     5641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5761 atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttata
     5881 gtccggaatc gtcacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat
     5941 tgcggccatc gtgatggcta gagtttagcc ctcccacaca taaccagagg gcagcaattc
     6001 acgaatccca actgccgtcg gctgtccatc actgtccttc actatggctt tgatcccagg
     6061 atgcagatcg agaagcacct gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc
     6121 atttccgatc gcgacgatac aagtcaggtt gccagctgcc gcagcagcag cagtgcccag
     6181 caccacgagt tctgcacaag gtcccccagt aaaatgatat acattgacac cagtgaagat
     6241 gcggccgtcg ctagagagag ctgcgctggc gacgctgtag tcttcagaga tggggatgct
     6301 gttgattgta gccgttgctc tttcaatgag ggtggattct tcttgagaca aaggcttggc
     6361 catggtggcg ctagtgtcag aagaatcgag ctttttgcaa aagcctaggc ctccaaaaaa
     6421 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     6481 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     6541 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     6601 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     6661 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     6721 cacattccac agctggttct ttccgcctca gaaggtacct aaccaagttc ctctttcaga
     6781 ggttatttca ggccatggtg ctgcgc
//
