LOCUS       sequence-423432-        7061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human GTF3C2. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_GTF3C2:1-911
FEATURES             Location/Qualifiers
     source          1..7061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4124..4245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4135..4154)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4360..4393)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4403..4450
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4482..5276
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4536..4555)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5146..5165
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5434..6022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5514..5533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6148..6196
                     /note="synthetic polyadenylation signal"
     CDS             complement(6220..6618)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6615..6624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6649..7006)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6663..6798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6815..7006
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6963..6983
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggatac ctgcggggtc
     1261 ggctatgttg ccctggggga ggccggcccc gtggggaaca tgactgtggt agactctcct
     1321 ggacaagagg tgctaaatca gcttgatgtc aagacctctt cagaaatgac cagtgcagag
     1381 gcttccgtag agatgtcatt acctacccct ttgcctggat ttgaggattc tcctgatcag
     1441 aggaggctcc ctccagagca ggaaagcctc tccagactgg aacagccaga tctttcttca
     1501 gagatgtcaa aggtctcaaa gcctagggcc tcaaagcctg gccggaagag aggtggtagg
     1561 acacgaaaag gccccaaaag gccccaacag cctaatcctc catcagcccc actggttcct
     1621 ggtctcttag atcaatccaa ccctctgtcc acccccatgc ctaagaaacg aggtcgaaag
     1681 tccaaggcag agctgctgct gctgaagttg tcaaaagacc tagatcggcc agaatctcaa
     1741 tctccaaaga ggccccctga ggactttgag accccttctg gggaacgacc ccgccgaagg
     1801 gctgcccaag tggcacttct gtatcttcag gaactggctg aagagctctc aacagccctg
     1861 cctgcccctg tgtcctgtcc tgagggcccc aaggtgagca gccccaccaa accgaagaag
     1921 atccggcagc cagcagcctg tccaggtgga gaagaggtgg atggtgctcc acgggatgaa
     1981 gacttttttc tccaggttga ggctgaagat gtggaagaaa gtgagggccc aagtgagagc
     2041 tcatctgaac ctgagcctgt agtgccccga agcaccccac gaggatctac ttcagggaaa
     2101 cagaaaccac actgccgagg aatggctccc aatggcttac cagatcatat catggctcct
     2161 gtttggaagt gcctccatct caccaaggac ttccgagagc agaaacattc atactgggag
     2221 tttgctgagt ggattccttt agcctggaag tggcacttgt tatctgagct tgaggccgct
     2281 ccctacctgc cccaggagga gaagtctcca ttgttttctg tacaacgtga agggctacct
     2341 gaagatggca ccctctaccg aataaacaga tttagctcga tcacagcaca tccagagcgc
     2401 tgggatgtgt ccttcttcac ggggggaccg ctctgggctc tggactggtg cccagtgcca
     2461 gagggggcag gagcctcgca atatgtggct cttttctcca gccctgacat gaatgagaca
     2521 cacccactga gccagcttca ttcgggtcct gggctgctcc agctctgggg ccttgggacc
     2581 ttgcagcaag aaagctgtcc tggcaacagg gcccactttg tctatgggat tgcttgtgac
     2641 aacggctgca tctgggacct caagttctgc cccagtggag catgggaact tccaggcacc
     2701 cctcggaagg ctcctctcct gccccggttg ggtctcttgg ctctggcctg ctcagacggg
     2761 aaagtactgc tattcagtct accccatccg gaggccctgc tggctcagca acccccagat
     2821 gcagtgaagc ctgccatata taaggtacaa tgtgtggcaa ctctgcaggt ggggtctatg
     2881 caagctacag acccctctga gtgtggtcag tgccttagcc tggcctggat gcctaccagg
     2941 ccccaccaac acctagctgc tggatattat aatggcatgg tggttttctg gaaccttccc
     3001 actaactcac ccctgcagcg gatacggctc tctgatggct ccttaaagct ctaccccttc
     3061 cagtgtttcc tagcccatga ccaggctgtg cgtacccttc aatggtgcaa agctaacagc
     3121 catttccttg tctctgcggg gagtgaccgg aaaatcaaat tctgggacct tcgacgtcct
     3181 tacgaaccca taaactctat caagcgcttc ttgagtacag aactggcctg gctgcttccc
     3241 tacaatggtg tcactgtggc tcaggacaac tgctatgcct cttatggact ctgtgggatt
     3301 cattatattg acgctggtta ccttggtttc aaggcctact tcactgctcc tcgaaaaggc
     3361 accgtttgga gtctttcagg atccgactgg cttgggacaa tagctgcagg agatatatcc
     3421 ggggagctca ttgctgctat attaccagat atggcactga atccaataaa tgtcaagcga
     3481 cctgtagagc gaagatttcc tatatataaa gcagatctga taccgtatca ggacagtcct
     3541 gaaggtccag accattcttc tgcttcatct ggggtcccca accctcctaa ggctcgaact
     3601 tacactgaaa ctgtcaacca tcactacttg ctctttcaag acacagattt gggttcattc
     3661 catgatctgc tccgtagaga accaatgctg cgcatgcagg agggagaggg gcattctcaa
     3721 ctctgcctgg acaggctgca gctggaggct attcataagg tacgtttcag cccaaacctg
     3781 gactcctatg gatggctggt atctgggggg cagtcagggc tggttcgaat ccattttgtc
     3841 cgtggactcg cctccccact gggccaccgt atgcagcttg aaagccgagc ccacttcaat
     3901 gctatgttcc aaccatcctc ccccactaga cggcctggct tctctccaac cagccatcgc
     3961 cttctgccca ctcccggctc gagcggtggg agctccggtg tgagcggctg gcggctgttc
     4021 aagaagatta gctaagttta aacggccgcg actctagact gcaggcatgc aagctgattt
     4081 ggctgctgcc accgctgagc aaggccgctt cgagcagaca tgataagata cattgatgag
     4141 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4201 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4261 attcatttta tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac
     4321 ctctacaaat gtggtaaaat cttaattaag acttctaata taacttcgta tagcatacat
     4381 tatacgaagt tatggttcca ctgttgacaa ttaatcatcg gcatagtata tcggcatagt
     4441 ataatacgac aaggtgagga actaaaccca ggaggcagat catgattgaa caagatggat
     4501 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4561 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4621 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     4681 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4741 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4801 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4861 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4921 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4981 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     5041 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5101 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5161 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5221 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgattcg
     5281 aaatgaccga ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg taatacggtt
     5341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     5881 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6001 gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6061 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6121 ctagatcctt ttatagtccg gaatcgtcac acaaaaaacc aacacacaga tgtaatgaaa
     6181 ataaagatat tttattgcgg ccatcgtgat ggctagagtt tagccctccc acacataacc
     6241 agagggcagc aattcacgaa tcccaactgc cgtcggctgt ccatcactgt ccttcactat
     6301 ggctttgatc ccaggatgca gatcgagaag cacctgtcgg caccgtccgc aggggctcaa
     6361 gatgcccctg ttctcatttc cgatcgcgac gatacaagtc aggttgccag ctgccgcagc
     6421 agcagcagtg cccagcacca cgagttctgc acaaggtccc ccagtaaaat gatatacatt
     6481 gacaccagtg aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc
     6541 agagatgggg atgctgttga ttgtagccgt tgctctttca atgagggtgg attcttcttg
     6601 agacaaaggc ttggccatgg tggcgctagt gtcagaagaa tcgagctttt tgcaaaagcc
     6661 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     6721 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     6781 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     6841 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     6901 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     6961 acaccctaac tgacacacat tccacagctg gttctttccg cctcagaagg tacctaacca
     7021 agttcctctt tcagaggtta tttcaggcca tggtgctgcg c
//
