LOCUS       sequence-423442-       10598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human NUP214. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_NUP214:1-2090
FEATURES             Location/Qualifiers
     source          1..10598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1730..1739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4388..4399
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    7661..7782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7672..7691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(7897..7930)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7940..7987
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8019..8813
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8073..8092)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8683..8702
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(8971..9559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9051..9070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    9685..9733
                     /note="synthetic polyadenylation signal"
     CDS             complement(9757..10155)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      10152..10161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10186..10543)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10200..10335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        10352..10543
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     10500..10520
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgggaga cgagatggat
     1261 gccatgattc ccgagcggga gatgaaggat tttcagttta gagcgctaaa gaaggtgaga
     1321 atctttgact cccctgagga attgcccaag gaacgctcga gtctgcttgc tgtgtccaac
     1381 aaatatggtc tggtcttcgc tggtggagcc agtggcttgc agatttttcc tactaaaaat
     1441 cttcttattc aaaataaacc cggagatgat cccaacaaaa tagttgataa agtccaaggc
     1501 ttgctagttc ctatgaaatt cccaatccat cacctggcct tgagctgtga taacctcaca
     1561 ctctctgcgt gcatgatgtc cagtgaatat ggttccatta ttgctttttt tgatgttcgc
     1621 acattctcaa atgaggctaa acagcaaaaa cgcccatttg cctatcataa gcttttgaaa
     1681 gatgcaggag gcatggtgat tgatatgaag tggaacccca ctgtcccctc catggtggca
     1741 gtttgtctgg ctgatggtag tattgctgtc ctgcaagtca cggaaacagt gaaagtatgt
     1801 gcaactcttc cttccacggt agcagtaacc tctgtgtgct ggagccccaa aggaaagcag
     1861 ctggcagtgg gaaaacagaa tggaactgtg gtccagtatc ttcctacttt gcaggaaaaa
     1921 aaagtcattc cttgtcctcc gttttatgag tcagatcatc ctgtcagagt tctggatgtg
     1981 ctgtggattg gtacctacgt cttcgccata gtgtatgctg ctgcagatgg gaccctggaa
     2041 acgtctccag atgtggtgat ggctctacta ccgaaaaaag aagaaaagca cccagagata
     2101 tttgtgaact ttatggagcc ctgttatggc agctgcacgg agagacagca tcattactac
     2161 ctcagttaca ttgaggaatg ggatttagtg ctggcagcat ctgcggcttc aacagaagtt
     2221 agtatccttg ctcgacaaag tgatcagatt aattgggaat cttggctact ggaggattct
     2281 agtcgagctg aattgcctgt gacagacaag agtgatgact ccttgcccat gggagttgtc
     2341 gtagactata caaaccaagt ggaaatcacc atcagtgatg aaaagactct tcctcctgct
     2401 ccagttctca tgttactttc aacagatggt gtgctttgtc cattttatat gattaatcaa
     2461 aatcctgggg ttaagtctct catcaaaaca ccagagcgac tttcattaga aggagagcga
     2521 cagcccaagt caccaggaag tactcccact accccaacct cctctcaagc cccacagaaa
     2581 ctggatgctt ctgcagctgc agcccctgcc tctctgccac cttcatcacc tgctgctccc
     2641 attgccactt tttctttgct tcctgctggt ggagccccca ctgtgttctc ctttggttct
     2701 tcatctttga agtcatcggc tacggtcact ggggagcccc cttcatattc cagtggctcc
     2761 gacagctcca aagcagcccc aggccctggc ccatcaacct tctcttttgt tcccccttct
     2821 aaagcctccc tagcccccac ccctgcagcg tctcctgtgg ctccatcagc tgcttcattc
     2881 tcctttggat catctggttt taagcctacc ctggaaagca caccagtgcc aagtgtgtct
     2941 gctccaaata tagcaatgaa gtcctccttc ccaccctcaa cctctgctgt caaagtcaac
     3001 cttagtgaaa agtttactgc tgcagctacc tctactcctg ttagtagctc ccagagcgca
     3061 cccccgatgt cgccattctc ttctgcctcc aagccagctg cttctggacc actcagccac
     3121 cccacacctc tctcagcacc acctagttcc gtgccattga agtcctcagt cttgccctca
     3181 ccatcaggac gatctgctca gggcagttca agcccagtgc cctcaatggt acagaaatca
     3241 cccaggataa cccctccagc ggcaaagcca ggctctcccc aggcaaagtc acttcagcct
     3301 gctgttgcag aaaagcaggg acatcagtgg aaagattcag atcctgtaat ggctggaatt
     3361 ggggaggaga ttgcacactt tcagaaggag ttggaagagt taaaagcccg aacttccaaa
     3421 gcctgtttcc aagtgggcac ttctgaggag atgaagatgc tgcgaacaga atcagatgac
     3481 ttgcatacct ttcttttgga gattaaagag accacagagt cgcttcatgg agatataagt
     3541 agcctgaaaa caactttact tgagggcttt gctggtgttg aggaagccag agaacaaaat
     3601 gaaagaaatc gtgactctgg ttatctgcat ttgctttata aaagaccact ggatcccaag
     3661 agtgaagctc agcttcagga aattcggcgc cttcatcagt atgtgaaatt tgctgtccaa
     3721 gatgtgaatg atgttctaga cttggagtgg gatcagcatc tggaacaaaa gaaaaaacaa
     3781 aggcacctgc ttgtgccaga gcgagagaca ctgtttaaca ccctagccaa caatcgggaa
     3841 atcatcaacc aacagaggaa gaggctgaat cacctggtgg atagtcttca gcagctccgc
     3901 ctttacaaac agacttccct gtggagcctg tcctcggctg ttccttccca gagcagcatt
     3961 cacagttttg acagtgacct ggaaagcctg tgcaatgctt tgttgaaaac caccatagaa
     4021 tctcacacca aatccttgcc caaagtacca gccaaactgt cccccatgaa acaggcacaa
     4081 ctgagaaact tcttggccaa gaggaagacc ccaccagtga gatccactgc tccagccagc
     4141 ctgtctcgat cagcctttct gtctcagaga tattatgaag acttggatga agtcagctca
     4201 acgtcatctg tctcccagtc tctggagagt gaagatgcac ggacgtcctg taaagatgac
     4261 gaggcagtgg ttcaggcccc tcggcacgcc cccgtggttc gcactccttc catccagccc
     4321 agtctcttgc cccatgcagc accttttgct aaatctcacc tggttcatgg ttcttcacct
     4381 ggtgtgatgg gaacttcagt ggctacatct gctagcaaaa ttattcctca aggggccgat
     4441 agcacaatgc ttgccacgaa aaccgtgaaa catggtgcac ctagtccttc ccaccccatc
     4501 tcagccccgc aggcagctgc cgcagcagca ctcaggcggc agatggccag tcaggcacca
     4561 gctgtaaaca ctttgactga atcaacgttg aagaatgtcc ctcaagtggt aaatgtgcag
     4621 gaattgaaga ataaccctgc aaccccttct acagccatgg gttcttcagt gccctactcc
     4681 acagccaaaa cacctcaccc agtgttgacc ccagtggctg ctaaccaagc caagcagggg
     4741 tctctaataa attcccttaa gccatctggg cctacaccag catccggtca gttatcatct
     4801 ggtgacaaag cttcagggac agccaagata gaaacagctg tgacttcaac cccatctgct
     4861 tctgggcagt tcagcaagcc tttctcattt tctccatcag ggactggctt taattttggg
     4921 ataatcacac caacaccgtc ttctaatttc actgctgcac aaggggcaac accctccact
     4981 aaagagtcaa gccagccgga cgcattctca tctggtgggg gaagcaaacc ttcttatgag
     5041 gccattcctg aaagctcacc tccctcagga atcacatccg catcaaacac caccccagga
     5101 gaacctgccg catctagcag cagacctgtg gcaccttctg gaactgctct ttccaccacc
     5161 tctagtaagc tggaaacccc accgtccaag ctgggagagc ttctgtttcc aagttctttg
     5221 gctggagaga ctctgggaag tttttcagga ctgcgggttg gccaagcaga tgattctaca
     5281 aaaccaacca ataaggcttc atccacaagc ctaactagta cccagccaac caagacgtca
     5341 ggcgtgccct cagggtttaa ttttactgcc cccccggtgt tagggaagca cacggagccc
     5401 cctgtgacat cctctgcaac caccacctca gtagcaccac cagcagccac cagcacttcc
     5461 tcaactgccg tttttggcag tctgccagtc accagtgcag gatcctctgg ggtcatcagt
     5521 tttggtggga catctctaag tgctggcaag actagttttt catttggaag ccaacagacc
     5581 aatagcacag tgcccccatc tgccccacca ccaactacag ctgccactcc ccttccaaca
     5641 tcattcccca cattgtcatt tggtagcctc ctgagttcag caactacccc ctccctgcct
     5701 atgtccgctg gcagaagcac agaagaggcc acttcatcag ctttgcctga gaagccaggt
     5761 gacagtgagg tctcagcatc agcagcctca cttctagagg agcaacagtc agcccagctt
     5821 ccccaggctc ctccgcaaac ttctgactct gttaaaaaag aacctgttct tgcccagcct
     5881 gcagtcagca actctggcac tgcagcatct agtactagtc ttgtagcact ttctgcagag
     5941 gctaccccag ccaccacggg ggtccctgat gccaggacgg aggcagtacc acctgcttcc
     6001 tccttttctg tgcctgggca gactgctgtc acagcagctg ctatctcaag tgcaggccct
     6061 gtggccgtcg aaacatcaag tacccccata gcctccagca ccacgtccat tgttgctccc
     6121 ggcccatctg cagaggcagc agcatttggt accgtcactt ctggctcatc cgtctttgct
     6181 cagcctcctg ctgccagttc tagctcagct ttcaaccagc tcaccaacaa cacagccact
     6241 gccccctctg ccacgcccgt gtttgggcaa gtggcagcca gcaccgcacc aagtctgttt
     6301 gggcagcaga ctggtagcac agccagcaca gcagctgcca caccacaggt cagcagctca
     6361 gggtttagca gcccagcttt tggtaccaca gccccagggg tctttggaca gacaaccttc
     6421 gggcaggcct cagtctttgg gcagtcggcg agcagtgctg caagtgtctt ttccttcagt
     6481 cagcctgggt tcagttccgt gcctgccttc ggtcagcctg cttcctccac tcccacatcc
     6541 accagtggaa gtgtctttgg tgccgcctca agtaccagta gctccagttc cttctcattt
     6601 ggacagtctt ctcccaacac aggagggggg ctgtttggcc aaagcaacgc tcctgctttt
     6661 gggcagagtc ctggctttgg acagggaggc tctgtctttg gtggtacctc agctgccacc
     6721 acaacagcag caacctctgg gttcagcttt tgccaagctt caggttttgg gtctagtaat
     6781 actggttctg tgtttggtca agcagccagt actggtggaa tagtctttgg ccagcaatca
     6841 tcctcttcca gtggtaacgt gtttgggtct ggaaacactg gaagaggggg aggtttcttc
     6901 agtggccttg gaggaaaacc cagtcaggat gcagccaaca aaaacccatt cagctcggcc
     6961 agtgggggct ttggatccac agctacctca aatacctcta acctatttgg aaacagtggg
     7021 gccaagacat ttggtggatt tgccagctcg tcgtttggag agcagaaacc cactggcact
     7081 ttcagctctg gaggaggaag tgtggcatcc caaggctttg ggttttcctc tccaaacaaa
     7141 acaggtggct tcggtgctgc tccagtgttt ggcagccctc ctacttttgg gggatcccct
     7201 gggtttggag gggtgccagc attcggttca gccccagcct ttacaagccc tctgggctcg
     7261 acgggaggca aagtgttcgg agagggcact gcagctgcca gcgcaggagg attcgggttt
     7321 gggagcagca gcaacaccac atccttcggc acgctcgcga gtcagaatgc ccccactttc
     7381 ggatcactgt cccaacagac ttctggtttt gggacccaga gtagcggatt ctctggtttt
     7441 ggatcaggca caggagggtt cagctttggg tcaaataact cgtctgtcca gggttttggt
     7501 ggctggcgaa gcggctcgag cggtgggagc tccggtgtga gcggctggcg gctgttcaag
     7561 aagattagct aagtttaaac ggccgcgact ctagactgca ggcatgcaag ctgatttggc
     7621 tgctgccacc gctgagcaag gccgcttcga gcagacatga taagatacat tgatgagttt
     7681 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     7741 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     7801 cattttatgt ttcaggttca gggggagatg tgggaggttt ttttaagcaa gtaaaacctc
     7861 tacaaatgtg gtaaaatctt aattaagact tctaatataa cttcgtatag catacattat
     7921 acgaagttat ggttccactg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7981 atacgacaag gtgaggaact aaacccagga ggcagatcat gattgaacaa gatggattgc
     8041 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     8101 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     8161 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     8221 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     8281 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     8341 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     8401 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     8461 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     8521 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     8581 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     8641 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     8701 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8761 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgattcgaaa
     8821 tgaccgacca agcgacgccc aaccggtatc agctcactca aaggcggtaa tacggttatc
     8881 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9541 cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9601 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9661 gatcctttta tagtccggaa tcgtcacaca aaaaaccaac acacagatgt aatgaaaata
     9721 aagatatttt attgcggcca tcgtgatggc tagagtttag ccctcccaca cataaccaga
     9781 gggcagcaat tcacgaatcc caactgccgt cggctgtcca tcactgtcct tcactatggc
     9841 tttgatccca ggatgcagat cgagaagcac ctgtcggcac cgtccgcagg ggctcaagat
     9901 gcccctgttc tcatttccga tcgcgacgat acaagtcagg ttgccagctg ccgcagcagc
     9961 agcagtgccc agcaccacga gttctgcaca aggtccccca gtaaaatgat atacattgac
    10021 accagtgaag atgcggccgt cgctagagag agctgcgctg gcgacgctgt agtcttcaga
    10081 gatggggatg ctgttgattg tagccgttgc tctttcaatg agggtggatt cttcttgaga
    10141 caaaggcttg gccatggtgg cgctagtgtc agaagaatcg agctttttgc aaaagcctag
    10201 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
    10261 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
    10321 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
    10381 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
    10441 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
    10501 ccctaactga cacacattcc acagctggtt ctttccgcct cagaaggtac ctaaccaagt
    10561 tcctctttca gaggttattt caggccatgg tgctgcgc
//
