LOCUS       sequence-423452-        6521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human STRN isoform 2. C-terminal
            HiBiT tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_STRN:1-731
FEATURES             Location/Qualifiers
     source          1..6521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3584..3705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3595..3614)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3820..3853)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3863..3910
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3942..4736
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3996..4015)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4606..4625
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4894..5482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4974..4993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5608..5656
                     /note="synthetic polyadenylation signal"
     CDS             complement(5680..6078)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6075..6084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6109..6466)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6123..6258
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6275..6466
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6423..6443
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggacga gcaggcgggt
     1261 cccggcgtct tcttcagcaa caaccacccg ggcgccggcg gtgccaaggg gctcgggcct
     1321 ctggcggggg cggcccgagc ccagtacagt ctcccgggga tcctgcactt cctgcagcac
     1381 gagtgggccc gcttcgaggt ggagagagcc cagtgggagg tggagcgggc ggagctgcag
     1441 gcccagattg ccttcctgca gggagaaagg aagggccaag aaaatttgaa gaaggatctt
     1501 gtgaggagga tcaaaatgtt ggagtatgct cttaaacagg aaagagccaa ataccacaag
     1561 ttgaaatacg ggacagaatt gaatcaggga gatatgaagc ctccaagcta tgattctgat
     1621 gaaggtaatg aaacagaagt gcagccacaa caaaacagcc agttaatgtg gaaacaaggt
     1681 cgacaactac tcagacagta tctacaggag gtgggttata cagatactat tctagatgtg
     1741 aaatctaaac gagtgcgagc tttgttgggc ttttcaagtg atgtcacgga cagggaagat
     1801 gacaaaaatc aggactcagt tgtaaatggc acagaggctg aagttaaaga gacagcaatg
     1861 attgcaaaat ctgagttaac agattctgcc tccgtgctgg ataatttcaa attccttgaa
     1921 agtgcagctg cagatttcag tgatgaagat gaagatgatg atgttgatgg aagagagaaa
     1981 agcgtcattg atacttcaac aattgttagg aaaaaagcat tgcctgacag cggtgaagat
     2041 cgagatacaa aagaagctct aaaggagttt gacttcttgg ttacatcaga ggaaggagac
     2101 aatgaatcta gaagtgcagg cgatggaaca gactgggggc ccaataggtc aaaactacaa
     2161 gatatgcttg ctaatttgag agatgttgat gaacttcctt cattgcagcc atctgtgggt
     2221 tcaccttcca gacccagcag ctccaggctt cctgaacatg aaattaatag ggcagatgaa
     2281 gtggaagcat tgacatttcc tccttcttct ggaaagtcat tcatcatggg agcagatgaa
     2341 gcccttgaaa gtgaactggg acttggagaa ctagcaggcc ttacggtggc caatgaagca
     2401 gactcactaa cttatgatat agcaaacaat aaagatgcat tgaggaagac atggaaccct
     2461 aagtttacat tgagaagtca ctttgatggc atccgagccc ttgctttcca tcccattgag
     2521 cctgttttga taacagcatc agaggatcac acattaaaaa tgtggaattt acagaaaaca
     2581 gccccagcca aaaagagcac ttctcttgat gtagaaccta tctatacatt cagagcccat
     2641 aaaggtccag tgctttgtgt ggtaatgagc agcaatggtg agcagtgtta cagtggtggt
     2701 actgatggac tgatccaggg ctggaatacc actaatccca acatcgaccc ctatgattct
     2761 tatgatcctt ctgttttacg aggccctctg ctaggccaca cggatgcagt ctggggtttg
     2821 gcttatagtg cagcacatca gcgtttgttg tcctgttcag cagatggcac tctgcgttta
     2881 tggaatacaa ctgaggttgc tccagcacta agtgtattta atgatactaa agaactggga
     2941 atccctgcct ctgtggatct agtgagcagt gacccgagcc atatggtagc atcattcagc
     3001 aagggatata caagcatttt taacatggaa acacaacaac gcattctcac tttagaatcc
     3061 aatgtagata caacagccaa ctcttcctgc caaataaata gagtcatcag tcatcctact
     3121 cttccgatca gcatcactgc tcatgaagac aggcacatca aattctatga taacaataca
     3181 ggcaaactga tccactcgat ggtagcccac ctagaagctg ttacaagttt agcagttgat
     3241 cccaatggcc tttacttgat gtctggcagt catgactgtt caatacgttt atggaatcta
     3301 gaaagtaaga cgtgtatcca agaattcaca gctcatcgaa aaaagtttga agaatcgatt
     3361 catgatgtag ctttccaccc atccaaatgc tatatagcca gtgctggagc tgacgcactg
     3421 gctaaagtct ttgtaggctc gagcggtggg agctccggtg tgagcggctg gcggctgttc
     3481 aagaagatta gctaagttta aacggccgcg actctagact gcaggcatgc aagctgattt
     3541 ggctgctgcc accgctgagc aaggccgctt cgagcagaca tgataagata cattgatgag
     3601 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3661 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3721 attcatttta tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac
     3781 ctctacaaat gtggtaaaat cttaattaag acttctaata taacttcgta tagcatacat
     3841 tatacgaagt tatggttcca ctgttgacaa ttaatcatcg gcatagtata tcggcatagt
     3901 ataatacgac aaggtgagga actaaaccca ggaggcagat catgattgaa caagatggat
     3961 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4021 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4081 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     4141 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4201 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4261 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4321 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4381 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4441 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     4501 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4561 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4621 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4681 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgattcg
     4741 aaatgaccga ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg taatacggtt
     4801 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4861 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4921 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4981 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5041 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5101 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5161 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5221 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5281 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     5341 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5401 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5461 gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5521 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5581 ctagatcctt ttatagtccg gaatcgtcac acaaaaaacc aacacacaga tgtaatgaaa
     5641 ataaagatat tttattgcgg ccatcgtgat ggctagagtt tagccctccc acacataacc
     5701 agagggcagc aattcacgaa tcccaactgc cgtcggctgt ccatcactgt ccttcactat
     5761 ggctttgatc ccaggatgca gatcgagaag cacctgtcgg caccgtccgc aggggctcaa
     5821 gatgcccctg ttctcatttc cgatcgcgac gatacaagtc aggttgccag ctgccgcagc
     5881 agcagcagtg cccagcacca cgagttctgc acaaggtccc ccagtaaaat gatatacatt
     5941 gacaccagtg aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc
     6001 agagatgggg atgctgttga ttgtagccgt tgctctttca atgagggtgg attcttcttg
     6061 agacaaaggc ttggccatgg tggcgctagt gtcagaagaa tcgagctttt tgcaaaagcc
     6121 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     6181 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     6241 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     6301 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     6361 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     6421 acaccctaac tgacacacat tccacagctg gttctttccg cctcagaagg tacctaacca
     6481 agttcctctt tcagaggtta tttcaggcca tggtgctgcg c
//
