LOCUS       sequence-423455-        6149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human ATG16L1. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_ATG16L1:1-607
FEATURES             Location/Qualifiers
     source          1..6149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3212..3333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3223..3242)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3448..3481)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3491..3538
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3570..4364
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3624..3643)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4234..4253
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4522..5110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4602..4621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5236..5284
                     /note="synthetic polyadenylation signal"
     CDS             complement(5308..5706)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5703..5712
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5737..6094)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5751..5886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5903..6094
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6051..6071
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgtcgtc gggcctccgc
     1261 gccgctgact tcccccgctg gaagcgccac atctcggagc aactgaggcg ccgggaccgg
     1321 ctgcagagac aggcgttcga ggagatcatc ctgcagtata acaaattgct ggaaaagtca
     1381 gatcttcatt cagtgttggc ccagaaacta caggctgaaa agcatgacgt accaaacagg
     1441 cacgagataa gtcccggaca tgatggcaca tggaatgaca atcagctaca agaaatggcc
     1501 caactgagga ttaagcacca agaggaactg actgaattac acaagaaacg tggggagtta
     1561 gctcaactgg tgattgacct gaataaccaa atgcagcgga aggacaggga gatgcagatg
     1621 aatgaagcaa aaattgcaga atgtttgcag actatctctg acctggagac ggagtgccta
     1681 gacctgcgca ctaagctttg tgaccttgaa agagccaacc agaccctgaa ggatgaatat
     1741 gatgccctgc agatcacttt tactgccttg gagggaaaac tgaggaaaac tacggaagag
     1801 aaccaggagc tggtcaccag atggatggct gagaaagccc aggaagccaa tcggcttaat
     1861 gcagagaatg aaaaagactc caggaggcgg caagcccggc tgcagaaaga gcttgcagaa
     1921 gcagcaaagg aacctctacc agtcgaacag gatgatgaca ttgaggtcat tgtggatgaa
     1981 acttctgatc acacagaaga gacctctcct gtgcgagcca tcagcagagc agccactaag
     2041 cgactctcgc agcctgctgg aggccttctg gattctatca ctaatatctt tgggagacgc
     2101 tctgtctctt ccttcccagt cccccaggac aatgtggatg ctcatcctgg ttctggtaaa
     2161 gaagtgaggg taccagctac tgccttgtgt gtcttcgatg cacatgatgg ggaagtcaac
     2221 gctgtgcagt tcagtccagg ttcccggtta ctggccactg gaggcatgga ccgcagggtt
     2281 aagctttggg aagtatttgg agaaaaatgt gagttcaagg gttccctatc tggcagtaat
     2341 gcaggaatta caagcattga atttgatagt gctggatctt acctcttagc agcttcaaat
     2401 gattttgcaa gccgaatctg gactgtggat gattatcgat tacggcacac actcacggga
     2461 cacagtggga aagtgctgtc tgctaagttc ctgctggaca atgcgcggat tgtctcagga
     2521 agtcacgacc ggactctcaa actctgggat ctacgcagca aagtctgcat aaagacagtg
     2581 tttgcaggat ccagttgcaa tgatattgtc tgcacagagc aatgtgtaat gagtggacat
     2641 tttgacaaga aaattcgttt ctgggacatt cgatcagaga gcatagttcg agagatggag
     2701 ctgttgggaa agattactgc cctggactta aacccagaaa ggactgagct cctgagctgc
     2761 tcccgtgatg acttgctaaa agttattgat ctccgaacaa atgctatcaa gcagacattc
     2821 agtgcacctg ggttcaagtg cggctctgac tggaccagag ttgtcttcag ccctgatggc
     2881 agttacgtgg cggcaggctc tgctgagggc tctctgtata tctggagtgt gctcacaggg
     2941 aaagtggaaa aggttctttc aaagcagcac agctcatcca tcaatgcggt ggcgtggtcg
     3001 ccctctggct cgcacgttgt cagtgtggac aaaggatgca aagctgtgct gtgggcacag
     3061 tacggctcga gcggtgggag ctccggtgtg agcggctggc ggctgttcaa gaagattagc
     3121 taagtttaaa cggccgcgac tctagactgc aggcatgcaa gctgatttgg ctgctgccac
     3181 cgctgagcaa ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     3241 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     3301 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3361 tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt
     3421 ggtaaaatct taattaagac ttctaatata acttcgtata gcatacatta tacgaagtta
     3481 tggttccact gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     3541 ggtgaggaac taaacccagg aggcagatca tgattgaaca agatggattg cacgcaggtt
     3601 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3661 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3721 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     3781 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3841 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     3901 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     3961 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4021 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4081 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4141 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4201 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4261 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4321 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgattcgaa atgaccgacc
     4381 aagcgacgcc caaccggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4921 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5101 aggatttcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5221 atagtccgga atcgtcacac aaaaaaccaa cacacagatg taatgaaaat aaagatattt
     5281 tattgcggcc atcgtgatgg ctagagttta gccctcccac acataaccag agggcagcaa
     5341 ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc ttcactatgg ctttgatccc
     5401 aggatgcaga tcgagaagca cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt
     5461 ctcatttccg atcgcgacga tacaagtcag gttgccagct gccgcagcag cagcagtgcc
     5521 cagcaccacg agttctgcac aaggtccccc agtaaaatga tatacattga caccagtgaa
     5581 gatgcggccg tcgctagaga gagctgcgct ggcgacgctg tagtcttcag agatggggat
     5641 gctgttgatt gtagccgttg ctctttcaat gagggtggat tcttcttgag acaaaggctt
     5701 ggccatggtg gcgctagtgt cagaagaatc gagctttttg caaaagccta ggcctccaaa
     5761 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     5821 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     5881 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     5941 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     6001 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     6061 acacacattc cacagctggt tctttccgcc tcagaaggta cctaaccaag ttcctctttc
     6121 agaggttatt tcaggccatg gtgctgcgc
//
