LOCUS       sequence-423456-        6188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human CFAP52. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_CFAP52:1-620
FEATURES             Location/Qualifiers
     source          1..6188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3251..3372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3262..3281)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3487..3520)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3530..3577
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3609..4403
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3663..3682)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4273..4292
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4561..5149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4641..4660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5275..5323
                     /note="synthetic polyadenylation signal"
     CDS             complement(5347..5745)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5742..5751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5776..6133)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5790..5925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5942..6133
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6090..6110
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggataa caaaatttcg
     1261 ccggaggccc aagtggcgga gctggaactt gacgccgtga tcggcttcaa tggacatgtg
     1321 cccactggtc tcaaatgcca tcctgaccag gagcatatga tttatcctct tggttgcaca
     1381 gtcctcattc aggcaataaa tactaaagag cagaacttcc tacagggtca tggcaacaac
     1441 gtctcctgct tggccatctc caggtctgga gagtacatcg cctccggaca agtcacattc
     1501 atggggttca aggtagacat cattttgtgg gattataaga acagagagct gcttgctcgg
     1561 ctgtcccttc acaaaggcaa aattgaagct ctggcctttt ctccaaatga tttgtacttg
     1621 gtatcactag gaggcccaga tgacggaagt gtggtggtgt ggagcatagc caagagagat
     1681 gccatctgtg gcagccctgc agccggcctc aatgttggca atgccaccaa tgtgatcttc
     1741 tccaggtgcc gggatgagat gtttatgact gctggaaatg ggacaattcg agtatgggaa
     1801 ttggatcttc caaatagaaa aatctggcca actgagagcc aaacaggaca gttgaaaaga
     1861 atagtcatga gtattggagt ggatgatgat gatagctttt tctaccttgg caccacgact
     1921 ggagatattc taaaaatgaa ccccaggact aaactgctga cagatgttgg gcctgcgaag
     1981 gacaaattca gtttgggagt gtcagctatc aggtgcctga agatgggggg tttgttggtg
     2041 ggctctggag ccggactgct ggtcttctgt aaaagccctg gctacaaacc catcaagaag
     2101 attcagttac aaggcggcat cacttctatc acacttcgag gagaaggaca ccagtttctc
     2161 gtaggaacag aagaatcgca catttatcgt gtcagcttca cggatttcaa agagacgctc
     2221 atagcgactt gtcactttga tgctgtcaag gatattgtct ttccatttgg cactgctgag
     2281 ctatttgcaa cctgtgccaa gaaggatatc agggtgtggc acacatcatc caacagggag
     2341 ctgctgcgga tcaccgtgcc caacatgacc tgccacggca tcgacttcat gagggacggc
     2401 aaaagcatca tttcagcatg gaacgacggt aaaatccgag ccttcgcccc agagacaggc
     2461 cgactgatgt atgtcattaa caatgctcac aggatcggcg tcaccgccat cgccaccacc
     2521 agtgactgta aaagggtcat cagtggcggt ggggaagggg aggtgagggt atggcagata
     2581 ggctgtcaga cccagaagct ggaggaggcc ctgaaggaac acaagtcatc agtgtcctgc
     2641 attagggtga agaggaacaa cgaggagtgt gtcaccgcca gcaccgatgg gacttgtatc
     2701 atttgggacc ttgtgcgtct caggaggaat cagatgatac tagccaacac cttattccag
     2761 tgtgtgtgct atcaccctga ggagttccag atcatcacca gcggaacaga cagaaagatt
     2821 gcttactggg aagtatttga tgggacagta atcagagaat tggaaggttc cctgtctggg
     2881 tcgataaatg gcatggatat cacacaggaa ggggtgcact ttgtcacagg tggaaatgac
     2941 catctggtca aagtttggga ttataatgag ggtgaagtga ctcacgttgg ggtgggacac
     3001 agtggcaaca tcacacgcat ccgcataagt ccaggaaatc aatatattgt tagtgtaagt
     3061 gccgatggag ccattttgcg atggaagtac ccatatacct ccggctcgag cggtgggagc
     3121 tccggtgtga gcggctggcg gctgttcaag aagattagct aagtttaaac ggccgcgact
     3181 ctagactgca ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga
     3241 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3301 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3361 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     3421 tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagact
     3481 tctaatataa cttcgtatag catacattat acgaagttat ggttccactg ttgacaatta
     3541 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga
     3601 ggcagatcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     3661 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     3721 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3781 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3841 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     3901 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3961 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4021 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4081 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     4141 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4201 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4261 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4321 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4381 gccttcttga cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc
     4441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4981 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
     5161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5221 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca
     5281 aaaaaccaac acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc
     5341 tagagtttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt
     5401 cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac
     5461 ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat
     5521 acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca
     5581 aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag
     5641 agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc
     5701 tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc
     5761 agaagaatcg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     5821 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     5881 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     5941 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     6001 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     6061 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     6121 ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg
     6181 tgctgcgc
//
