LOCUS       sequence-423485-        9732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For knock-in donor vector by CRISPR/CAS system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R
  TITLE     One-Step Generation of Mice Carrying Reporter and Conditional 
            Alleles by CRISPR/Cas-Mediated Genome Engineering.
  JOURNAL   Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 
            10.1016/j.cell.2013.08.022.
  PUBMED    23992847
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Aug 27. pii: S0092-8674(13)01016-7. doi: 
            10.1016/j.cell.2013.08.022."
COMMENT     Sequence Label: Nanog-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..9732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2310..2366
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2367..3077
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2512..2530)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2780..2799
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6165..6184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6166..6184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6191..6208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6191..6207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6200..6222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6348..6776
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6435..6454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6645..6666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7120..7914
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7174..7193)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7784..7803
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(7932..8000,8001..8792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8150..8169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8963..9551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9452..9471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9705..9722
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 caggctctga agacctgcct cttcaaggca gccctgattc ttctaccagt cccaaacaaa
      361 agctctcaag tcctgaggct gacaagggcc ctgaggagga ggagaacaag gtccttgcca
      421 ggaagcagaa gatgcggact gtgttctctc aggcccagct gtgtgcactc aaggacaggt
      481 ttcagaagca gaagtacctc agcctccagc agatgcaaga actctcctcc attctgaacc
      541 tgagctataa gcaggtgggg tccttagaag ttgctgtaat gtaacacaat gagactgact
      601 tgctaatgaa tcgaactaac gtctggacgt ctctctaact atcctatcct cctatcctat
      661 gcgcctgtta aaatcataat tatgtgattg attgattgat tgattatgta taagtacact
      721 atagctgtct tcagatacac cagaagaggg catcagatct cattacggat ggttgtgagc
      781 caccatgtgg ttgctgggat ttgaactcag gaccttcgga agagcagtca gtgctcttaa
      841 ccgctgagcc atctctccag ccccaattat gtgatttata tatttcccag tttgtttcat
      901 tggttacttt atcttctact tcacctccca cacccatccc caaatcaggg tctcactgta
      961 gccctgactg gtctctgtag accaggctgg gagaggttca cctgcctctg tctcctgggt
     1021 gctggaaata aaggtgtgcc tggctttttt cctttctttt cttttttaag attgtttatt
     1081 tttaaattcc tttagttcat tggtgttttg cctgcatgtg tttgtctttg tgagagtatc
     1141 agatctcctg gactagagtt acagacagtc gtgagctgcc atgtggccgc tgagaattga
     1201 acccaggtcc tctggaagag cagccagtgc tcttaacaga agagccattt cccccagcac
     1261 ctgtcttttt caagaaagag tttcacttta caggcctggc tgtcatgttt aaggtcgggc
     1321 tgtagacctg tctgcattct gtatgtctta gcttgttcga atgtcctgtc actctgcagg
     1381 ttaagacctg gtttcaaaac caaaggatga agtgcaagcg gtggcagaaa aaccagtggt
     1441 tgaagactag caatggtctg attcaggtaa caagatgttt tctctgcttt cctttttccc
     1501 cctccatctt accatcttat tttatgtgtc tgtgccgtgt gtgtgtgtgt gtgtgtgtgt
     1561 gtggctgacc tatggctgtt gggtatggat cccagatttc ctgtaagagc agtcagtggc
     1621 tctgcctttg cttcccagtg tcatcctgat ccatctatgt ccattagtcc tgtccctttc
     1681 ccatccctct tcctccacgc actctttctt gtgatctttg gttctttgag gcaggctctc
     1741 tctatgtagc cctggcagtc agagatctgc ctgcctagtc tcaggagtgc tggggttaac
     1801 ggcctgtgcg gccatgctaa tcttttcagt atttctgcag tttacatctt tagagtcccc
     1861 ttttcactca ttgcattaat cacctctttc agaagggctc agcaccagtg gagtatccca
     1921 gcatccattg cagctatccc cagggctatc tggtgaacgc atctggaagc ctttccatgt
     1981 ggggcagcca gacttggacc aacccaactt ggagcagcca gacctggacc aacccaactt
     2041 ggaacaacca gacctggacc aacccaactt ggagcagcca ggcctggacc gctcagtcct
     2101 ggaacggcca gccttggaat gctgctccgc tccataactt cggggaggac tttctgcagc
     2161 cttacgtaca gttgcagcaa aacttctctg ccagtgattt ggaggtgaat ttggaagcca
     2221 ctagggaaag ccatgcgcat tttagcaccc cacaagcctt ggaattattc ctgaactact
     2281 ctgtgactcc accaggtgaa atagctagcg ccactaactt ctccctgttg aaacaagcag
     2341 gggatgtcga agagaatccc gggccaatgg tgagcaaggg cgaggaggat aacatggcca
     2401 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     2461 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     2521 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     2581 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     2641 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     2701 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     2761 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     2821 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     2881 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     2941 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     3001 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     3061 acgagctgta caagtaatta gcgccgactt acgcaacatc tgggcttaaa gtcagggcaa
     3121 agccaggttc cttccttctt ccaaatattt tcatattttt tttaaagatt tatttattca
     3181 ttatatgtaa gtacactgta gctgtcttca gacactccag aagagggcgt cagatcttgt
     3241 tacgtatggt tgtgagccac catgtggttg ctgggatttg aactcctgac cttcggaaga
     3301 gcagtcgggt gctcttatcc actgagccat ctcaccagcc cctggtttat ttttttaatt
     3361 attatttgct ttttgtttat caagacaggg tttctctgca tagctctaat tgtctttgaa
     3421 ctagctctgc agaccagcct ggccttgaac tcagagatct gcccacttat ctttgcctcc
     3481 tgaatgctgg gaccaaaggt ggcataccac cacacctggc atatatattg tttatttcta
     3541 tttctatttt tattggtgcc agagcaaacc taggacttag aacatgctgg gcaccaactc
     3601 aacttctgag ctctatttac aacttggtgt gttagtgtat ttgtcttagt tctgaatttg
     3661 tccttttttt agtgttaact ctaggctttg gagacagtga ggtgcatata ctctctcctt
     3721 cccaagaata agtgcttgaa cacccttacc cacgcccacc cacccatgct agtctttttt
     3781 cttagaagcg tgggtcttgg tatacactgt gtcattttga ggggtgaggt ttaaaagtat
     3841 atacaaagta taacgatatg gtggctactc tcgaggatga gacagaagga ccaggagttt
     3901 gagggtagct cagatatgca ataagttcaa ggccaacctg tactatgttt aaatagtaag
     3961 acagcatctc gataaaataa taaaactaaa gtctcaacaa aataaaagct ttcacctatt
     4021 aaggtgcttg cttgtccttg gagtccccca agagtaactg ctatgttaat atctgtagaa
     4081 agatgtttat atttgactgt accatgatga accgatgcca gctggactag tttaaacaaa
     4141 ataaaacact aattttacct ttagtggtct tatcttttgt ccagtggggt caatatactt
     4201 tttattttgg tatgtgaagg ggagggtctt aaccgagggt ccaggaaaat gggtttcttt
     4261 tggcttaacc ggttgggagg gttataccac aacagttgga atggcctggt aaccagagca
     4321 ggaggccagc cactcacaat gcatccagtc aagaactgga gagcaatgaa taagaaatga
     4381 atgttttagt gtgggtatgg gccctgagga ggccagaagc attcgatgat cccctggaga
     4441 tgaagttacg tgaagccacc tgatcacctg atgtggcagg ctgcagggaa ataagctcta
     4501 gttctctgga agaacaaata tttaactggg agagtggttt ttattcctgt gaaataatag
     4561 aacttgtgga gagagcccct gaggtcacac acctagtgct tctgggaaag aggagggacc
     4621 acagtctctt gtttttgtgt cacactggag attaatctgg gacttcctgg ctccagatgg
     4681 aggctatctt ccctgagcat aaccccctaa cctacgtggg ggtgggggaa gatgtcaagt
     4741 gttatcacag gagacttgag aggctgtgtg ggttcagaag tgagtttggg gttttggaga
     4801 cgatttaaat ttgctgaaaa gacagaagat acgtatacat ctggtgtagt tgtgtgtaca
     4861 cttaaggaga ttaagttaac gtaactcctg atgcattaat tatagatgtt atagtagttg
     4921 tatacagtag atttagctaa agcatgatac ggaaaaacac agaagaagca agcgacacct
     4981 agtaactgcc gtgcgtaaat agccatacct gtcaatcaca tctttaactc ctgaccactc
     5041 acaggggtac ccagcccagc ccaagtaggt tctgtagctg ctcctcagct aaggagaggg
     5101 ctgttttggc agtgagaaaa attgtccctc attgtgccac agaggctagg acacattccc
     5161 ttgcaaagcc cagactaatt ccacctagaa ttctcctctt ctgctttctg tcctttggca
     5221 tcagtgggga tggctgctgt gtcagagatg ggacatcatc acctccacaa accctgacga
     5281 ccgttactga gagaccactt tgcttcaact cttagaggtt tcgttgataa gattctaaaa
     5341 tgtgtggtct tggggatcaa atccagggac tcttagaccc tgggtaagtg ctctactact
     5401 gaacaacact cagtccttgg tgtgagaatc cgagctttga ttttaccctt tttagggtgg
     5461 aagagattcc accaatcacc caggagtggg gctagttcaa gaggcctccc cagctgcagc
     5521 tttcagaggc tttttgcttc aggctcccac caagggacct attcaaatgc cttagtcaac
     5581 tgacatctga gtcccaagct gttgcaggac cccagctgac ttctttattt aacaaatcct
     5641 cctttttccc ctgctcgggc tcctctccct cctctgtctg gcaactgtcc gctttctctt
     5701 tgtagttccc caccaggacc tgttttctct ccactgtcac caggttttct gccgactggg
     5761 tttattcaca acagaattaa ccttttcctt tacccagaga agccagccct tggaattcaa
     5821 tggcagagaa ggtctgtgct tctgagcttg tagtttgcac tgtccaattc cgaagaccca
     5881 ggcaaatttg atccagggtg ctccgtgtta gacctgagga agaaaccaat tccaagactg
     5941 cctgattaaa gcaggtttta cttagaggaa cagcaggaac tggccagccc atgcacatga
     6001 gtaaaacaag cttggttgga ttaatttggc cacgatggtt tgcgttagtg gtcttccttc
     6061 tcccaacttt gggattgaca tttgcggccg gccaaaaggg cgaattctgc agatatccat
     6121 cacactggcg gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt
     6181 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6241 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6301 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
     6361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6781 caaaattcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag
     6841 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
     6901 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
     6961 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
     7021 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
     7081 tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     7141 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     7201 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     7261 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     7321 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     7381 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atcccacctt
     7441 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     7501 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     7561 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     7621 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     7681 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     7741 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     7801 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     7861 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattgaa
     7921 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7981 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8041 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8101 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8161 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8221 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8281 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8341 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8401 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8461 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8521 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8581 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8641 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8701 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8761 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8821 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8881 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9241 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9301 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9361 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9421 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9481 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9541 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9601 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9661 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9721 aggaagcgga ag
//
