LOCUS       sequence-423487-       12904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutagenesis of the Arabidopsis MAR1 gene for efficient screening..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Cheng Y, Zhang L, Ke Y, Dang X, Miki D
  TITLE     Double step screening using endogenous marker improves relative gene
            targeting efficiency in Arabidopsis.
  JOURNAL   Sci Rep. 2024 Dec 28;14(1):30791. doi: 10.1038/s41598-024-80352-y.
  PUBMED    39730554
REFERENCE   2  (bases 1 to 12904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-12-28"; volume: "14(1):30791. doi"; pages: " 
            10.1038/s41598-024-80352-y"
COMMENT     Sequence Label: pCambia3301-AtU6-MAR1
FEATURES             Location/Qualifiers
     source          1..12904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2..16,207..2024)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          17..206
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2031..2048
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2083..2335
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2357..2381
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3682..4311
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4740..5813
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5879..6073
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6309..6331)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6417..6557
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6472..6491
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6572..6589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6743..7331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6823..6842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8756..9550)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9975..9999
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10077..10251
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10258..10809)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10860..11205)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    11396..11417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11432..11437,11438..11455,11456..11462)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11470..11486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11475..11497
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11494..11510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11494..11510
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        complement(11552..11627)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    12091..12147
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(12151..12168)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12151..12167)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12160..12182)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        12544..12889
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
      961 tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg
     1021 tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct
     1081 actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg
     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     1261 ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca
     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2401 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     2461 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     2521 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     2581 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     2641 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     2701 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     2761 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     2821 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     2881 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     2941 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     3001 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     3061 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     3121 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     3181 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     3241 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     3301 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgt cctggcggcc gccgagaatg
     3361 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     3421 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     3481 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     3541 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     3601 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     3661 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     3721 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     3781 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     3841 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     3901 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     3961 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     4021 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     4081 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     4141 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     4201 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     4261 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     4321 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     4381 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     4441 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     4501 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     4561 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     4621 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     4681 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     4741 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     4801 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     4861 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     4921 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     4981 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     5041 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     5101 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     5161 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     5221 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     5281 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     5341 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     5401 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     5461 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     5521 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     5581 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     5641 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     5701 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     5761 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     5821 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
     5881 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     5941 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     6001 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     6061 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     6121 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     6181 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     6241 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     6301 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6361 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     6421 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     6481 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6541 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7141 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7381 actcacgtta agggctgatg aatcccctaa tgatttttat caaaatcatt aagttaaggt
     7441 agatacacat cttgtcatat gatcaaatgg tttcgccaaa aatcaataat cagacaacaa
     7501 aatgtgcgaa ctcgatattt tacacgactc tctttaccaa ttctgccccg aattacactt
     7561 aaaacgactc aacagcttaa cgttggcttg ccacgcctta cttgactgta aaactctcac
     7621 tcttaccgaa cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg
     7681 aatcgaccga ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg
     7741 gcatgctagc tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga
     7801 tatccgtgag caaaaacggc ttatggtatt gcgagcttca gtcgcactac acggtcgttc
     7861 tgttactctt tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga
     7921 ccaatttcta gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag
     7981 tgatgctggc tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt
     8041 aagtcgagta agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat
     8101 cagcaactta catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac
     8161 taaaagcaac ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa
     8221 aaatcagcgc tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc
     8281 ggcaaaggag ccatggattc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca
     8341 acttgttaat atctattcga agcgaatgca gattgaagaa accttccgag acttgaaaag
     8401 tcctgcctac ggactaggcc tacgccatag ccgaacgagc agctcagagc gttttgatat
     8461 catgctgcta atcgccctga tgcttcaact aacatgttgg cttgcgggcg ttcatgctca
     8521 gaaacaaggt tgggacaagc acttccaggc taacacagtc agaaatcgaa acgtactctc
     8581 aacagttcgc ttaggcatgg aagttttgcg gcattctggc tacacaataa caagggaaga
     8641 ctcactcgtg gctgcaaccc tgcttactca aaatctattc acacatggtt acgttttggg
     8701 gaaattatga ggggatctct cagcgttaag ggattttggt catgcattct aggtactaaa
     8761 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     8821 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
     8881 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     8941 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     9001 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     9061 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     9121 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     9181 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     9241 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     9301 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     9361 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     9421 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     9481 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     9541 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     9601 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
     9661 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     9721 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     9781 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     9841 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     9901 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     9961 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
    10021 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc
    10081 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
    10141 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
    10201 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagtca
    10261 aatctcggtg acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca
    10321 gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg
    10381 ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc
    10441 gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga
    10501 gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc
    10561 gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac
    10621 ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg
    10681 cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat
    10741 ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc
    10801 tgggctcatg gtagactcga gagagataga tttgtagaga gagactggtg atttcagcgt
    10861 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
    10921 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    10981 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    11041 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    11101 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    11161 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    11221 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
    11281 gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11341 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11401 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11461 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattaca
    11521 ctagtaattg cccttcgaag ggacaaaaaa agcaccgact cggtgccact ttttcaagtt
    11581 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacctg gagttctgga
    11641 ccgtcctcaa tcactacttc gactctagct gtatataaac tcagcttcgt tttcttatct
    11701 aagcgatgtg ggacttttga agattgtttt caacttaaat gggcctatat aagaaatact
    11761 attgttcttt cccatataaa tgggcctgct tctcttcttt cagattccca ggggcctttt
    11821 gaagattatc ttcatatctt aagaatgaag atgttttatt caatcaaatt cttgaaggtt
    11881 cgatgcctaa tcattctaat cctgggacaa actatgaaac aagatacaaa aactccgaat
    11941 ggaaagttaa aaagaagaaa acgaaagcta cggttcaaga aaatgtaagc tgataaacaa
    12001 aaaaaaactg tatgaacgaa gaagaagaaa aaaagctaag aagaaatgat gtattgtgcg
    12061 gaaggcaagt cgagtttccg ttgttcaacg gaattcgagc tcggtacccg gggatcctct
    12121 agagtcgacc tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg
    12181 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    12241 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    12301 cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttagctt
    12361 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
    12421 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
    12481 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
    12541 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
    12601 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
    12661 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
    12721 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
    12781 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
    12841 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct
    12901 tgac
//
