LOCUS       sequence-423492-        6391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red norepinephrine indicator nLightR in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Kagiampaki Z, Rohner V, Kiss C, Curreli S, Dieter A, Wilhelm M, 
            Harada M, Duss SN, Dernic J, Bhat MA, Zhou X, Ravotto L, Ziebarth T,
            Wasielewski LM, Sonmez L, Benke D, Weber B, Bohacek J, Reiner A, 
            Wiegert JS, Fellin T, Patriarchi T
  TITLE     Sensitive multicolor indicators for monitoring norepinephrine in 
            vivo.
  JOURNAL   Nat Methods. 2023 Sep;20(9):1426-1436. doi: 
            10.1038/s41592-023-01959-z.
  PUBMED    37474807
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2023-09"; volume: "20"; issue: "9"; pages: 
            "1426-1436"
COMMENT     Sequence Label: pAAV_hSyn1_nLightR
FEATURES             Location/Qualifiers
     source          1..6391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        825..1272
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          1296..1428
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1411..1430
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    3431..4019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3484..3504)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3902..3913)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4037..4261
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4304..4444
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4304..4433
                     /label=AAV2 ITR
     rep_origin      4519..4974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4606..4625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4816..4837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4991..5010)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5110..5132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5170..5188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5256..5360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5361..5429,5430..6221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5579..5598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca agtgggtttt
      841 aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac
      901 aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac
      961 aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc
     1021 ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc
     1081 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
     1141 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
     1201 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
     1261 ctgagagcgc agtcgagatt aattaactcg agcaggtaag tatcaaggtt acaagacagg
     1321 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
     1381 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gctagcgttt
     1441 aaacggtacc ggatcctccg ccaccatgaa gacgatcatc gccctgagct acatcttctg
     1501 cctggtgttc gccgactaca aggacgatga tgacgcagtc tttctcagtg ggaacgctag
     1561 tgactcttca aattgtaccc acccaccacc tcctgttaat atcagcaagg ctattttgct
     1621 gggtgtcatc ttgggaggcc tgattctgtt cggagtgctt ggtaacattc tcgtaattct
     1681 ctcagtagcg tgtcaccgcc atctccacag cgttacccac tactatattg tgaacctcgc
     1741 tgtagcagac ctgcttctca cgagtacggt actgccattt tccgctatct tcgaaatttt
     1801 ggggtattgg gcctttggac gggttttttg taacatctgg gccgctgtgg acgtattgtg
     1861 ttgtacggca tcaatcatgg gcctgtgcct tatctcaata gatcgatact gggcgattag
     1921 ttcacccgag cgctacgaga gaaagatgac tccgaaggcg gcgttcattg ctctcctttg
     1981 tgtctgggct ctttccttgg ttatatccat tgggcccttg ttcggctggc gccagcccgc
     2041 cccggaggac gaaacaatat gtcagatcaa cgaagagcct ggatacgtcc ttttcagcgc
     2101 cctcggatct ttttatgtcc cgttgacaat catcctggtc atgtattgca gagtttatcg
     2161 gatcgcgcag aaacagctct ccagcttcca cgtgagcgag aggatgtatc ctgaggacgg
     2221 tgcgctgaaa tccgaaataa agaaaggcct gcgcctcaaa gacgggggcc actacgccgc
     2281 tgaggttaag acaacttaca aggctaaaaa gcctgtccag ctcccgggcg cctacatagt
     2341 cgacattaaa ctggacatag tcagccataa cgaggactac accatagtcg aacagtgtga
     2401 gcgggcggaa ggtcgccaca gtacaggagg aatggacgaa ttgtataaag ggggcaccgg
     2461 tggcagcttg gttagcaagg gagaggaaga taacatggct attataaagg agttcatgag
     2521 atttaaagtt cacatggagg gatctgtaaa cgggcatgag tttgagattg agggagaagg
     2581 agaagggcga ccgtacgagg cgttccaaac cgcgaaattg aaggtcacca agggaggacc
     2641 ccttccgttt gcttgggaca ttcttagccc acaattcatg tatggcagca aggcgtacat
     2701 aaagcacccg gcagacatac ccgactactt caaacttagc tttccagagg gtttccgatg
     2761 ggaaagagtg atgaactttg aggatggtgg catcatacac gtcaatcaag atagcagcct
     2821 tcaggacgga gtatttatat acaaagtcaa actccggggt acaaactttc ccccggacgg
     2881 gccagtcatg cagaagaaga ctatgggttg ggaggcgcgg aaagacgact tgaagaggga
     2941 gactaaagtc ctcaagacgc tcgggatagt tgttggttgt ttcgtactct gctggttgcc
     3001 ctttttcttg gtgatgccga taggtagctt ttttcccgac ttccgaccga gtgagacagt
     3061 attcaaaata gctttttggc ttggttattt gaactcctgt attaatccca ttatatatcc
     3121 ctgttcatct caagagttta aaaaggcttt tcaaaacgtg ttgcgcatac aatgcctgcg
     3181 cagaaagcag tcttctaagc acgcactcgg ttatacgctc caccccccat cccatgctct
     3241 cgagggacag tatagaaacc tcgtgcgaat acctgtgggt agtggcgaaa cattttgcaa
     3301 aatcagtaaa acagacgggg tatgtgaatg gaaatttttt tcatccttgc cacgcggatc
     3361 tgccagaata acagttccta aggaccagtc cgcttgcaca actgcccggg acttttaaaa
     3421 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3481 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3541 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3601 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3661 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3721 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3781 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     3841 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3901 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     3961 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgct
     4021 ctcgagatac gtacgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4081 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4141 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4201 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4261 gcttctgagg cggaaagatc tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     4321 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4381 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4441 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4501 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4561 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     4621 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4681 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4741 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4801 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     4861 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     4921 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4981 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5041 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5101 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5161 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5221 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5281 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5341 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5401 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5461 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5521 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5581 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5641 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5701 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5761 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5821 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5881 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5941 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6001 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6061 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6121 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6181 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6241 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6301 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6361 cagaccccgt agaaaagatc aaaggatctt c
//
