LOCUS       sequence-42350-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00442
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(570..669)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(685..702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(685..701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(694..716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct
       61 tataatgcca actttgtaca agaaagttgg caaaaatggc gttttggccg cgctgccttg
      121 ctgctcctcc agtttgatgc gcttgcactt catgcgtcga ttttggaacc acaccttcac
      181 ctggcgttcg gtcagctcca gcgccactgc gatttcgtag cgacgcaagc gagtcaaata
      241 gttggaatag cagaactccg cttccagctg cttgagctgc gtttttgaaa atgctgtgcg
      301 ttccttgcga ttcgagctga ttgcccggag tttggacctt tggcttggaa gttcttgacc
      361 agtaggtgcc tcattttgta accattgatc gttcgcttcg ttgcaccaat tagtttctag
      421 tttattgtaa tccgcgtagg aagaagacgc actgtggtca tatacatagc tgcagtccgt
      481 atagccctcc gtttggttca gccacgattt cgtaatatcc tcgtagcaat aggattcttg
      541 gtagacgttc gcgtgggagt tcttcatggt gccaactttt ttgtacaaag ttggcattat
      601 aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
      661 cattatttgg ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
      721 catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata
//
