LOCUS       sequence-42352-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TPK1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             810..827
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctctgaata ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac
       61 agcagcggca gagaaaactt gtatttccag ggcgggaatt tgaagtactg ccttgtaatt
      121 cttaatcagc ctttggacaa ctattttcgt catctttgga acaaagctct tttaagagcc
      181 tgtgccgatg gaggtgccaa ccgcttatat gatatcaccg aaggagagag agaaagcttt
      241 ttgcctgaat tcatcaatgg agactttgat tctattaggc ctgaagtcag agaatactat
      301 gctactaagg gatgtgagct catttcaact cctgatcaag accacactga ctttactaag
      361 tgccttaaaa tgctccaaaa gaagatagaa gaaaaagact taaaggttga tgtgatcgtg
      421 acactgggag gccttgctgg gcgttttgac cagattatgg catctgtgaa taccttgttc
      481 caagcgactc acatcactcc ttttccaatt ataataatcc aagaggaatc gctgatctac
      541 ctgctccaac caggaaagca caggttgcat gtagacactg gaatggaggg tgattggtgt
      601 ggccttattc ctgttggaca gccttgtatg caggttacaa ccacaggcct caagtggaac
      661 ctcacaaatg atgtgcttgc ttttggaaca ttggtcagta cttccaatac ctacgacggg
      721 tctggtgttg tgactgtgga aactgaccac ccactcctct ggaccatggc catcaaaagc
      781 tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggct
//
