LOCUS       sequence-423528-        1397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrative fluorescent reporter for amino acid biosynthesis sensing
            pathways, comprising GFP driven by the ARG8 promoter within the Easy
            clone vector, requires NotI digestion for integration in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Paukstyte J, Tena EC, Saarikangas J
  TITLE     A dual reporter system for intracellular and extracellular amino 
            acid sensing in budding yeast.
  JOURNAL   Mol Biol Cell. 2025 Apr 2:mbcE24040162. doi: 
            10.1091/mbc.E24-04-0162.
  PUBMED    40172974
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2025 Apr 2:mbcE24040162. doi: 10.1091/mbc.E24-04-0162."
COMMENT     Sequence Label: pCfB2188-PrARG8-GFP::URA3
FEATURES             Location/Qualifiers
     source          1..1397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caaggaacac ccccggtaat ggcgtgcgat tcctgttagc aggaatgtgt cacactttct
       61 acaactattt ctagtaaata accagctact gcttattgga ggtagaaagg caccacacca
      121 aggtctctct gataattgga tatttgatat gaaaacaaga gagtggtcga tgatcaagag
      181 tttatcacac acgagattca gacatagtgc atgtagctta ccggatggaa acgtcctgat
      241 ccttggtggg gttacagaag gacctgccat gctgctatac aatgtcaccg aagaaatctt
      301 caaagacgta actccaaaag atgaattctt ccaaaattcg ctagtatcag cgggtctaga
      361 atttgatccg gtttccaaac aaggcataat attgggtggt ggatttatgg atcaaacaac
      421 tgtctcggac aaggcaatta ttttcaaata tgatgcggaa aatgctactg aaccaattac
      481 ggtgattaag aagctccagc accccttatt tcaacgttat ggatcccaga taaaatacat
      541 tactccaaga aagctattaa tactcggtgg aacaagtcct tccgggctat ttgaccggac
      601 caactctata atcagccttg atccgctgag tgagacgcta acatcaattc ccatatcaag
      661 acgcatatgg gaagatcact cactaatgct tgctggattt agtttggtgt ccacttctat
      721 gggcaccata catatcattg gaggcggtgc cacttgctac ggctttggat cagtgactaa
      781 tgtaggtctt aagctcatag caatcgcgaa ataatgctcc tatcgggatt cgaatggttg
      841 ccagctcgct atgtgactca cttaaagtac atgatgcgtc attgtgcact tttttacaaa
      901 ataatggcct ttttaccgct ctatttaaaa gcgtgaaaaa aaaattgcaa catgaaagag
      961 aattcaactc ttaagtgcaa gtgactgcca acattttttt cgtttgttag aataattcaa
     1021 gaatcgctac caatcatctg tcataaaaca atgtccaaag gggaagaatt attccctggg
     1081 ggttgcccca attttggttg aattagaggg ggatgttaag ggaccaaaat ttttctgtcc
     1141 cccggggaag gggaagggga agcttccttt cgggaaaatt gaccttaaaa tttatttgga
     1201 cccctgggaa aattgccagt tcccgggccc aaccttaacc caccactttt cggttaaggg
     1261 ggtcaaaggt tgtggggaaa atccccaaaa tttttaaaaa aaaaaatgag tttttttaag
     1321 ttttcccgcg gccaaaaggg gttttttttc aaaaaaagac attttttttt ttaaaagagg
     1381 gggggttccc caagacc
//
