LOCUS       sequence-423529-        1360 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrative fluorescent reporter for amino acid biosynthesis sensing
            pathways, comprising GFP driven by the ARG8 promoter within the Easy
            clone vector, requires NotI digestion for integration in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Paukstyte J, Tena EC, Saarikangas J
  TITLE     A dual reporter system for intracellular and extracellular amino 
            acid sensing in budding yeast.
  JOURNAL   Mol Biol Cell. 2025 Apr 2:mbcE24040162. doi: 
            10.1091/mbc.E24-04-0162.
  PUBMED    40172974
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2025 Apr 2:mbcE24040162. doi: 10.1091/mbc.E24-04-0162."
COMMENT     Sequence Label: pCfB2188-PrARG8-GFP::URA3
FEATURES             Location/Qualifiers
     source          1..1360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctgggacga ttatttgtaa ttcatccata ccatgggtaa taccagcagc agtaacaaat
       61 tctaacaaga ccatgtggtc tctcttttcg tttggatctt tggataaggc agattgagtg
      121 gataagtaat ggttgtctgg taacaagact ggaccatcac caattggagt attttgttga
      181 taatggtcag ctaattgaac agaaccatct tcaatgttgt gtctaatttt gaagttaact
      241 ttgataccat tcttttgttt gtcagccatg atgtaaacat tgtgagagtt atagttgtat
      301 tccaatttgt gacctaaaat gttaccatct tctttaaaat caataccttt taattcgatt
      361 ctattaacta aggtatcacc ttcaaacttg acttcagctc tggtcttgta gttaccgtca
      421 tctttgaaaa aaatagttct ttcttgaaca taaccttctg gcatggcaga cttgaaaaag
      481 tcatgttgtt tcatatgatc tgggtatctc gcaaaacatt gaacaccata accgaaagta
      541 gtgactaagg ttggccatgg aactggcaat ttaccagtag tacaaataaa ttttaaggtc
      601 aatttaccgt aagtagcatc accttcacct tcaccggaga cagaaaattt gtgaccatta
      661 acatcaccat ctaattcaac caaaattggg acaacaccag tgaataattc ttcaccttta
      721 gacattgttt tatgacagat gattggtagc gattcttgaa ttattctaac aaacgaaaaa
      781 aatgttcgca gtcacttgca cttaagagtc gaattttctt catgttgcaa tttttttttc
      841 acgcttttaa atagagcgga aaaaggccat tatttcgtaa aaaagtgcac aatgacgcat
      901 catgtacttt aagtgagtca catagcgagc tggcaaccat tcgaatcccg ataggagcat
      961 tatttcgcga ttgctatgag cttaagacct acattagtca ctgatccaaa gccgtagcaa
     1021 gtggcaccgc ctccaatgat atgtatggtg cccatagaag tggacaccaa actaaatcca
     1081 gcaagcatta ttgagtgatc ttcccatatg cgtcttgaaa tgggaattga tgttagcgtc
     1141 tccctcagcg gatcaaggct gattaagaag ttggtccggt caaaaagccc ggaaggactt
     1201 gttccaccga ctataaaaac ctttttttgg aaaaaagggt tttttttcgg gaacccaaaa
     1261 cttaaaaaaa ggggggtggg actttttaac cccgaaatgg gtcaaaaaaa tttttccccc
     1321 caaatttaaa aaatttcctt ttccaaaaaa attttttttt
//
