LOCUS       sequence-42353-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: DDI1
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             513..530
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(583..601)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      597..644
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(768..787)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggttccgcgt ggtagtggac aagtgacgat gctctacatt
      121 aactgcaaag tgaatgggca tcctttgaag gcttttgttg actcgggcgc ccagatgacc
      181 attatgagcc aggcttgtgc cgagcgatgt aacatcatga ggctggtgga ccgacggtgg
      241 gctggggttg ctaaaggagt gggcacacag agaattattg gccgtgttca tctagctcag
      301 attcaaattg aaggtgattt cttacagtgc tctttctcca tacttgagga tcaacccatg
      361 gatatgcttc taggcctaga tatgctccgg agacatcaat gttccatcga tttgaagaaa
      421 aatgtgctgg tcatcggcac cactggcacg cagacttatt ttcttcctga gggagagttg
      481 ccctgatgac gaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg
      541 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      601 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      661 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      721 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      781 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atc
//
