LOCUS       sequence-423555-        2072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TEF promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Jiang W, Wang S, Avila P, Jorgensen TS, Yang Z, Borodina I
  TITLE     Combinatorial iterative method for metabolic engineering of Yarrowia
            lipolytica: application for betanin biosynthesis.
  JOURNAL   Metab Eng. 2024 Sep 9:S1096-7176(24)00110-1. doi: 
            10.1016/j.ymben.2024.09.003.
  PUBMED    39260817
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2024 Sep 9:S1096-7176(24)00110-1. doi: 10.1016/j.ymben.2024.09.003."
COMMENT     Sequence Label: pCfB13089
FEATURES             Location/Qualifiers
     source          1..2072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(40..74)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(145..804)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     719..738
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      986..1574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1475..1494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgagtataa gaatcattca aaaatgtgag accagaccaa taaaaaacgc ccggcggcaa
       61 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
      121 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
      181 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
      241 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      301 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      361 attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      421 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      481 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      541 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
      601 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      661 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      721 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      781 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
      841 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
      901 cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
      961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1621 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtagtcggt
     1681 ctcaacggag agaccgggtt ggcggcgcat ttgtgtccca aaaaacagcc ccaattgccc
     1741 caattgaccc caaattgacc cagtagcggg cccaaccccg gcgagagccc ccttctcccc
     1801 acatatcaaa cctcccccgg ttcccacact tgccgttaag ggcgtagggt actgcagtct
     1861 ggaatctacg cttgttcaga ctttgtacta gtttctttgt ctggccatcc gggtaaccca
     1921 tgccggacgc aaaatagact actgaaaatt tttttgcttt gtggttggga ctttagccaa
     1981 gggtataaaa gaccaccgtc cccgaattac ctttcctctt cttttctctc tctccttgtc
     2041 aactcacacc cgaaatcgtt aagcatttcc tt
//
