LOCUS       sequence-423563-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike N439K for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 N439K (AAA)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          642..1117
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    5014..5602
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5067..5087)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5485..5496)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5655..5776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5666..5685)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5720..5739
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      5891..5937
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      5967..6103
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6148..6219
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        6264..6460
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6311..6446
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6474..7268
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6528..6547)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7138..7157
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7279..7503
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7699..8287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7779..7798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9033..9101,9102..9893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9251..9270)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      9906..10042
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    10194..10265
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(10270..10316)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    10334..10382
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tcccctcgaa
      601 gcttacatgt ggtaccgagc tcggatcctg agaacttcag ggtgagtcta tgggaccctt
      661 gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta
      721 acagggtaca catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct
      781 ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc
      841 tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag
      901 tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt ctgcatataa
      961 attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc
     1021 tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt
     1081 gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg tgctggtctg
     1141 tgtgctggcc catcactttg gcaaaggcgg ccgccatgtt tgtgttcctc gttctcctgc
     1201 ccctcgtttc ttcccaatgc gtgaacctca caactagaac gcagctccca cccgcttaca
     1261 cgaactcttt tactcgtgga gtatactatc ccgataaagt ctttaggtcc agtgtccttc
     1321 attctactca agaccttttc ctgccattct tctcaaatgt cacctggttc catgctatcc
     1381 acgtttctgg caccaacgga actaaacgat ttgataatcc cgtcttgcca tttaacgatg
     1441 gcgtatactt tgcctcaacg gagaagtcca acattatccg gggatggata tttggcacca
     1501 ccctggactc caagacacag tctctcctca ttgtgaacaa cgccaccaat gtcgtcatca
     1561 aagtctgtga atttcaattt tgtaacgatc cctttctcgg ggtttattat cataagaata
     1621 acaagtcttg gatggagtca gagttccgcg tttacagttc cgctaacaac tgtacttttg
     1681 agtacgtttc ccaaccattt ctcatggatc ttgaagggaa gcaaggcaac ttcaagaatc
     1741 tccgcgaatt tgtgtttaag aatatagacg gctacttcaa aatttatagc aagcataccc
     1801 cgataaatct ggttcgggat ctccctcaag gcttctccgc gttggaaccc ctcgtggact
     1861 tgccgattgg catcaacatc acccggttcc aaacccttct tgcccttcat cgaagctacc
     1921 tcacgcctgg cgacagtagt agtggttgga cagcgggcgc agcagcctac tatgtgggat
     1981 atttgcaacc acgcaccttt cttctcaaat ataacgaaaa cggaacaatt accgatgccg
     2041 tagactgtgc cctcgatccc ctgtccgaaa ctaaatgcac actcaagtcc ttcacagttg
     2101 agaaaggcat ataccaaacc agtaacttcc gcgtccaacc caccgaatcc attgttcgat
     2161 tccctaacat caccaatttg tgtcctttcg gtgaagtatt taatgccact cgatttgcat
     2221 cagtttatgc ctggaatcgc aagcgaatat caaattgtgt tgcagattat agtgtcctgt
     2281 ataatagcgc atcattcagc acttttaagt gttatggggt cagccctacg aaattgaatg
     2341 acttgtgttt taccaacgtt tacgcagatt ctttcgtcat acgcggcgac gaagtccgac
     2401 agattgcacc ggggcaaacc ggaaagatag cagactataa ttataagttg ccggatgact
     2461 tcaccggctg cgttatagca tggaactcca aaaatctcga ttcaaaagtc ggcggcaact
     2521 ataattatct ttaccgcctg tttcgcaagt caaatctgaa gccctttgaa cgtgacatct
     2581 ccaccgaaat ttatcaagct ggcagcaccc catgtaatgg agtggaaggg tttaactgtt
     2641 attttcccct tcaatcatac ggatttcaac caacaaatgg ggtgggctat caaccatatc
     2701 gggtggtagt cttgagcttt gagctgctcc acgcaccggc tacagtttgc ggaccgaaga
     2761 aaagcactaa cctggtgaag aataaatgtg tgaacttcaa ctttaatggg ctcaccggaa
     2821 caggggtgct gacagaatca aacaagaaat ttctcccttt tcaacaattt ggtagagaca
     2881 ttgccgatac cacagatgcc gtccgcgatc cgcaaacact ggaaatcctc gatataacac
     2941 cctgtagctt cggcggggtc agcgtgataa ctcccggaac gaacacatcc aaccaagtgg
     3001 ctgtcctgta tcaagatgtc aactgtaccg aagtccctgt ggcgatacac gccgaccaac
     3061 tgacacccac ctggagagtc tactcaacag gttccaatgt ctttcaaact agagcgggtt
     3121 gtctcattgg cgctgagcat gtcaataatt catacgaatg tgatattcct attggggcag
     3181 gaatctgtgc gtcttatcaa actcaaacga actcaccacg gcgcgctcgc tcagtagcca
     3241 gccagtccat aatcgcctat accatgagtc tcggggctga gaactccgtg gcttattcaa
     3301 acaattccat cgcaatccca actaacttta ccattagcgt taccacggaa atcttgcccg
     3361 tcagcatgac taagacgagc gtagactgta cgatgtatat ctgtggggat tccaccgagt
     3421 gtagcaacct cttgcttcaa tacgggtctt tctgtactca actcaaccga gcattgaccg
     3481 gaattgcggt cgaacaagac aagaataccc aagaggtgtt cgcacaagta aagcaaatat
     3541 acaaaacgcc gccaatcaaa gattttggcg ggtttaattt tagccaaata ctgccagacc
     3601 catctaagcc ttcaaaacgc agctttatcg aggatctgct gttcaacaaa gttacgttgg
     3661 ctgacgcggg tttcattaag cagtatgggg attgtttggg agacattgcg gcccgcgacc
     3721 ttatctgtgc tcaaaaattc aatgggttga cagttctgcc ccctctgctc actgatgaga
     3781 tgattgcaca gtatacatca gcattgttgg caggaaccat tacatccggc tggacttttg
     3841 gcgcgggtgc ggcccttcaa atacctttcg ctatgcaaat ggcttacaga ttcaatggga
     3901 ttggtgtcac gcaaaatgta ttgtatgaaa atcagaagct gatcgctaat caatttaaca
     3961 gcgcgattgg caagatccaa gattcactgt caagcacagc gtccgccctc ggcaaactcc
     4021 aagatgttgt gaatcaaaac gcccaggccc tgaataccct cgtgaagcaa ctgagttcta
     4081 acttcggcgc aatctccagc gtcctcaacg acatattgtc cagacttgat aaggtggaag
     4141 ccgaggtgca aattgatcgg ctcatcacag gcaggctcca gtcactccaa acttatgtga
     4201 cacaacagtt gatccgggct gctgagatac gagcaagcgc caacctggcc gccactaaaa
     4261 tgtcagagtg cgtgctcggg cagtccaaac gggttgactt ttgtggcaag ggctatcact
     4321 tgatgtcctt cccccaatcc gccccacatg gagtggtctt tctccacgtc acttatgtcc
     4381 ctgctcaaga gaagaacttt acgacagctc cagcaatctg tcacgatggc aaagcgcact
     4441 ttcccaggga aggcgttttc gtcagtaatg gcacgcattg gtttgtgacc caaaggaatt
     4501 tctatgaacc ccaaatcata accactgaca atacatttgt ctcaggcaac tgtgatgtgg
     4561 tcattgggat cgtgaacaac acggtatatg atcccctgca acccgagttg gatagcttca
     4621 aagaggaatt ggataaatac tttaagaatc atacttcacc cgatgttgat ctcggggaca
     4681 tatcaggaat aaatgcaagt gtcgtcaaca ttcagaaaga gattgatcgc cttaatgagg
     4741 tcgccaagaa cttgaatgaa tctttgatcg acttgcaaga actgggcaaa tacgaacaat
     4801 atattaaatg gccctggtac atttggttgg gctttatcgc cggacttata gctatcgtta
     4861 tggtcacaat tatgttgtgc tgtatgacta gctgttgttc ctgcttgaaa ggatgttgca
     4921 gttgcggatc ttgttgtaaa taaaggatcc tctagaagtt gtctcctcct gcactgactg
     4981 actgatacaa tcgatttctg gatccgcagg cctaatcaac ctctggatta caaaatttgt
     5041 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5101 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5161 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5221 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5281 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5341 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5401 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     5461 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5521 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5581 tccctttggg ccgcctcccc gcctgtctag cttgactgac tgagatacag cgtaccttca
     5641 gctcacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     5701 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     5761 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     5821 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtattg gcccatctct
     5881 atcggtatcg tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgtgc
     5941 ccctcgggcc ggattgctat ctaccggcat tggcgcagaa aaaaatgcct gatgcgacgc
     6001 tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg
     6061 cccacttcca tacgtgtcct ccttaccaga aatttatcct taaggtcgtc agctatcctg
     6121 caggcgatct ctcgatttcg atcaagacat tcctttaatg gtcttttctg gacaccacta
     6181 ggggtcagaa gtagttcatc aaactttctt ccctccctaa tctcattggt taccttgggc
     6241 tatcgaaact taattaagcg atctgcatct caattagtca gcaaccatag tcccgcccct
     6301 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatcgctg
     6361 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6421 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa ggaggtagcc aacatgattg
     6481 aacaagatgg attgcacgca ggttctcccg ccgcttgggt ggagaggcta ttcggctatg
     6541 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     6601 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctccaggacg
     6661 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6721 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6781 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6841 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6901 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6961 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc gacggcgagg
     7021 atctcgtcgt gacccacggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     7081 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     7141 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     7201 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     7261 tcttctagta tgtaagccct gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     7321 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     7381 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     7441 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     7501 tggttaatta accagtcaag tcagctactt ggcgagatcg acttgtctgg gtttcgacta
     7561 cgctcagaat tgcgtcagtc aagttcgatc tggtccttgc tattgcaccc gttctccgat
     7621 tacgagtttc atttaaatca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8101 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8521 tgcatttaaa tttccgaact ctccaaggcc ctcgtcggaa aatcttcaaa cctttcgtcc
     8581 gatccatctt gcaggctacc tctcgaacga actatcgcaa gtctcttggc cggccttgcg
     8641 ccttggctat tgcttggcag cgcctatcgc caggtattac tccaatcccg aatatccgag
     8701 atcgggatca cccgagagaa gttcaaccta catcctcaat cccgatctat ccgagatccg
     8761 aggaatatcg aaatcggggc gcgcctggtg taccgagaac gatcctctca gtgcgagtct
     8821 cgacgatcca tatcgttgct tggcagtcag ccagtcggaa tccagcttgg gacccaggaa
     8881 gtccaatcgt cagatattgt actcaagcct ggtcacggca gcgtaccgat ctgtttaaac
     8941 ctagatattg atagtctgat cggtcaacgt ataatcgagt cctagctttt gcaaacatct
     9001 atcaagagac aggatcagca ggaggctttc gcatgagtat tcaacatttc cgtgtcgccc
     9061 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     9121 aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg ttacatcgaa ctggatctca
     9181 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ctttccaatg atgagcactt
     9241 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9301 gtcgccgcat acactattct cagaatgact tggttgagta ttcaccagtc acagaaaagc
     9361 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9421 acactgcggc caacttactt ctgacaacga ttggaggacc gaaggagcta accgcttttt
     9481 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9541 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca accttgcgta
     9601 aactattaac tggcgaacta cttactctag cttcccggca acagttgata gactggatgg
     9661 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9721 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9781 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9841 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taaccgattc
     9901 taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta tactcccaca
     9961 tatgccagat tcagcaacgg atacggcttc cccaacttgc ccacttccat acgtgtcctc
    10021 cttaccagaa atttatcctt aagatcccga atcgtttaaa ctcgactctg gctctatcga
    10081 atctccgtcg tttcgagctt acgcgaacag ccgtggcgct catttgctcg tcgggcatcg
    10141 aatctcgtca gctatcgtca gcttaccttt ttggcagcga tcgcggctcc cgacatcttg
    10201 gaccattagc tccacaggta tcttcttccc tctagtggtc ataacagcag cttcagctac
    10261 ctctcaattc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctatcaa
    10321 tcgttgcgtt acacacacaa aaaaccaaca cacatccatc ttcgatggat agcgatttta
    10381 ttatctaact gctgatcgag tgtagccaga tctagtaatc aattacgggg tcattagttc
    10441 atagcccata tatggagttc cg
//
