LOCUS       sequence-423571-       10089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wild-type ARNT in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Chakraborty AA, Laukka T, Myllykoski M, Ringel AE, Booker MA, 
            Tolstorukov MY, Meng YJ, Meier SR, Jennings RB, Creech AL, Herbert 
            ZT, McBrayer SK, Olenchock BA, Jaffe JD, Haigis MC, Beroukhim R, 
            Signoretti S, Koivunen P, Kaelin WG Jr
  TITLE     Histone demethylase KDM6A directly senses oxygen to control 
            chromatin and cell fate.
  JOURNAL   Science. 2019 Mar 15;363(6432):1217-1222. doi: 
            10.1126/science.aaw1026. Epub 2019 Mar 14.
  PUBMED    30872525
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaw1026"; journalName: "Science"; date: 
            "2019-03-15- 15"; volume: "363"; issue: "6432"; pages: "1217-1222"
COMMENT     Sequence Label: pLX304-ARNT
FEATURES             Location/Qualifiers
     source          1..10089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2988..3012)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3014..3055
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3097..3685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3150..3170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3568..3579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3757..3990
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4068..4189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4105..4124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4159..4178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4229..4364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4384..4403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4385..4403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4413..4430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4413..4429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4422..4444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4571..5026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4658..4677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4868..4889
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5052..5156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5157..5225,5226..6017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5375..5394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6188..6776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6677..6696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6930..6947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7064..7085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7100..7105,7106..7123,7124..7130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7138..7154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7143..7165
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7162..7178
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7162..7178
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7197..7217
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7199..7217
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7245..7471
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7472..7652
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7699..7824
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8317..8550
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8735..8779
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8954..9454
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9373..9391
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9466..9861
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9921..10038
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagttggca tggcagcgac tactgccaac cccgaaatga catcagatgt
      661 accatcactg ggtccagcca ttgcctctgg aaactctgga cctggaattc aaggtggagg
      721 agccattgtc cagagggcta ttaagcggcg accagggctg gattttgatg atgatggaga
      781 agggaacagt aaatttttga ggtgtgatga tgatcagatg tctaacgata aggagcggtt
      841 tgccaggtcg gatgatgagc agagctctgc ggataaagag agacttgcca gggaaaatca
      901 cagtgaaatt gaacggcggc gacggaacaa gatgacagcc tacatcacag aactgtcaga
      961 tatggtaccc acctgtagtg ccctggctcg aaaaccagac aagctaacca tcttacgcat
     1021 ggcagtttct cacatgaagt ccttgcgggg aactggcaac acatccactg atggctccta
     1081 taagccgtct ttcctcactg atcaggaact gaaacatttg atcttggagg cagcagatgg
     1141 ctttctgttt attgtctcat gtgagacagg cagggtggtg tatgtctctg actccgtgac
     1201 tcctgttttg aaccagccac agtctgaatg gtttggcagc acactctatg atcaggtgca
     1261 cccagatgat gtggataaac ttcgtgagca gctttccact tcagaaaatg ccctgacagg
     1321 gcgtatcctg gatctaaaga ctggaacagt gaaaaaggaa ggtcagcagt cttccatgag
     1381 aatgtgtatg ggctcaagga gatcgtttat ttgccgaatg aggtgtggca gtagctctgt
     1441 ggacccagtt tctgtgaata ggctgagctt tgtgaggaac agatgcagga atggacttgg
     1501 ctctgtaaag gatggggaac ctcacttcgt ggtggtccac tgcacaggct acatcaaggc
     1561 ctggccccca gcaggtgttt ccctcccaga tgatgaccca gaggctggcc agggaagcaa
     1621 gttttgccta gtggccattg gcagattgca ggtaactagt tctcccaact gtacagacat
     1681 gagtaatgtt tgtcaaccaa cagagttcat ctcccgacac aacattgagg gtatcttcac
     1741 ttttgtggat caccgctgtg tggctactgt tggctaccag ccacaggaac tcttaggaaa
     1801 gaatattgta gaattctgtc atcctgaaga ccagcagctt ctaagagaca gcttccaaca
     1861 ggtagtgaaa ttaaaaggcc aagtgctgtc tgtcatgttc cggttccggt ctaagaacca
     1921 agaatggctc tggatgagaa ccagctcctt tactttccag aacccttact cagatgaaat
     1981 tgagtacatc atctgtacca acaccaatgt gaagaactct agccaagaac cacggcctac
     2041 actctccaac acaatccaga ggccacaact aggtcccaca gctaatttac ccctggagat
     2101 gggctcagga cagctggcac ccaggcagca gcaacagcaa acagaattgg acatggtacc
     2161 aggaagagat ggactggcca gctacaatca ttcccaggtg gttcagcctg tgacaaccac
     2221 aggaccagaa cacagcaagc cccttgagaa gtcagatggt ttatttgccc aggatagaga
     2281 tccaagattt tcagaaatct atcacaacat caatgcggat cagagtaaag gcatctcctc
     2341 cagcactgtc cctgccaccc aacagctatt ctcccagggc aacacattcc ctcctacccc
     2401 ccggccggca gagaatttca ggaatagtgg cctagcccct cctgtaacca ttgtccagcc
     2461 atcagcttct gcaggacaga tgttggccca gatttcccgc cactccaacc ccacccaagg
     2521 agcaacccca acttggaccc ctactacccg ctcaggcttt tctgcccagc aggtggctac
     2581 ccaggctact gctaagactc gtacttccca gtttggtgtg ggcagctttc agactccatc
     2641 ctccttcagc tccatgtccc tccctggtgc cccaactgca tcgcctggtg ctgctgccta
     2701 ccctagtctc accaatcgtg gatctaactt tgctcctgag actggacaga ctgcaggaca
     2761 attccagaca cggacagcag agggtgtggg tgtctggcca cagtggcagg gccagcagcc
     2821 tcatcatcgt tcaagttcta gtgagcaaca tgttcaacaa ccgccagcac agcaacctgg
     2881 ccagcctgag gtcttccagg agatgctgtc catgctggga gatcagagca acagctacaa
     2941 caatgaagaa ttccctgatc taactatgtt tccccccttt tcagaatgcc caactttctt
     3001 gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta
     3061 atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     3121 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3181 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3241 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3301 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3361 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3421 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3481 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3541 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3601 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3661 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     3721 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3781 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3961 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     4021 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4141 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4201 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4261 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4321 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4381 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4441 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4501 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4561 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4621 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4681 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4741 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4801 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4861 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4921 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4981 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5161 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5221 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5281 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5341 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5401 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5461 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5521 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5581 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5641 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5701 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5761 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5821 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5881 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5941 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6001 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6301 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6901 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6961 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7021 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7081 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7141 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     7201 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7261 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     7321 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     7381 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     7441 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7501 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7561 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7621 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7681 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7741 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7801 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7861 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7921 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7981 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8041 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8101 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8161 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8221 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8281 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     8341 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8401 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8461 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8521 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8581 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     8641 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     8701 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     8761 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     8821 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     8881 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     8941 cccgagggga cccttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     9001 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     9061 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     9121 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     9181 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     9241 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     9301 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     9361 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     9421 cctcgttgac cgaatcaccg acctctctcc ccagggggta ccaccatggc caagcctttg
     9481 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     9541 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     9601 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     9661 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     9721 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     9781 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     9841 tctggttatg tgtgggaggg cctgcagctg cagtagtaag aattctagat cttgagacaa
     9901 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     9961 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
    10021 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
    10081 ctcgagggg
//
