LOCUS       sequence-423573-        8089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses one of the split transcription factor component in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Shen J, Geng L, Li X, Emery C, Kroning K, Shingles G, Lee K, Heyden 
            M, Li P, Wang W
  TITLE     A general method for chemogenetic control of peptide function.
  JOURNAL   Nat Methods. 2023 Jan;20(1):112-122. doi: 
            10.1038/s41592-022-01697-8. Epub 2022 Dec 8.
  PUBMED    36481965
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01697-8"; journalName: "Nat Methods"; date: 
            "2023-01"; volume: "20"; issue: "1"; pages: "112-122"
COMMENT     Sequence Label: plx208-CMV-SspB-EGFP-VP16
FEATURES             Location/Qualifiers
     source          1..8089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    121..142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(157..162,163..180,181..187)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    195..211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     200..222
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     219..235
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     219..235
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     254..274
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        256..274
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        302..528
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             529..709
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    756..881
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1374..1607
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1792..1836
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2144..2261
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2378..2681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2682..2885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2836..2856
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(3253..3255,3256..3258,3259..3969)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3253..3255,3256..3258,3259..3969)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3298..3319)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3559..3578)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3906..3927
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3970..3990
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3991..4224
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     misc_feature    4243..4831
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4296..4316)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4714..4725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4903..5136
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5214..5335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5251..5270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5305..5324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5375..5510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5530..5549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5531..5549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5559..5576)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5559..5575)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5568..5590)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5717..6172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5804..5823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6014..6035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6198..6302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6303..6371,6372..7163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6521..6540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7334..7922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7823..7842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(8076..8089,1..4)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
       61 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      121 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      181 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      241 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      301 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      361 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      421 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      481 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      721 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      781 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      841 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      901 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      961 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1021 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1081 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1141 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1201 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1261 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1321 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1381 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1441 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1501 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1561 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1621 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1681 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1741 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1801 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1861 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1921 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1981 cagacccacc tcccaacccc gagagcgctg ccgtctggac cgatggctgt gtggaagtgc
     2041 tcgccgatag tggaaacaga cgccccagca ctcgtcctag ggcaaaggat ctgcagtaat
     2101 gagaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2161 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2221 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2281 gcagagatcc actttggcgc cggctcgagg gggcccgggg ttattaatag taatcaatta
     2341 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2401 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2461 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2521 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2581 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2641 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2701 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2761 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2821 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2881 gagctctctg gctaactgtc gggatccacc atgagccaac tgactcctcg aaggccatat
     2941 ttgctgaggg ccttttatga atggctcctc gataatcaac ttaccccaca cttggtggta
     3001 gatgtaaccc ttcctggcgt acaggtaccc atggaatacg ccagagacgg tcaaattgta
     3061 cttaatattg ccccacgcgc cgttggcaac cttgagctgg caaacgatga ggtccgcttt
     3121 aacgcacggt ttggaggcat tccacgacaa gtatccgtgc ctctggccgc cgtacttgct
     3181 atatacgccc gcgagaatgg agccggcacc atgttcgagc cagaagccgc ctatgatggt
     3241 agtgggggag gaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3301 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3361 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3421 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3481 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3541 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3601 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3661 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3721 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     3781 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3841 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     3901 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3961 ctgtacaagc ccaagaagaa gaggaaggtg gcccccccga ccgatgtcag cctgggggac
     4021 gaattgcact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc
     4081 gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc
     4141 gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc
     4201 cttggaattg acgagtacgg tgggtagacg cgttaagtcg acaatcaacc tctggattac
     4261 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4321 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4381 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4441 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4501 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4561 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4621 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4681 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4741 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4801 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     4861 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4921 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4981 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5041 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5101 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     5161 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5341 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5401 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5461 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     5521 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     5581 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5641 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5701 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5761 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5821 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5881 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5941 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6001 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6061 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6121 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6181 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6241 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6301 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6361 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6421 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6481 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6541 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6601 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6661 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6721 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6781 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6841 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6901 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6961 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7021 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7081 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7141 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7201 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7261 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7441 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7741 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8041 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgag
//
