LOCUS       sequence-423575-        9679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1329
FEATURES             Location/Qualifiers
     source          1..9679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2712..3300
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2765..2785)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3183..3194)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3372..3605
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3683..3804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3720..3739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3774..3793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3844..3979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3999..4018)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4000..4018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4028..4045)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4028..4044)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4037..4059)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4186..4641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4273..4292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4483..4504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4667..4771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4772..4840,4841..5632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4990..5009)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5803..6391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6292..6311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6545..6562
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6679..6700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6715..6720,6721..6738,6739..6745)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6753..6769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6758..6780
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6777..6793
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6777..6793
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6812..6832
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6814..6832
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6860..7086
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7087..7267
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7314..7439
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7932..8165
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8350..8394
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8625..8835
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8647..8843
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8694..8829
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8875..9477
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8875..8894)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9371..9391
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9520..9637
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag caccgccacc atgctttgga aattgacgga taatatcaag
     1321 tacgaggact gcgaggaccg tcacgacggc accagcaacg ggacggcacg gttgccccag
     1381 ctgggcactg taggtcaatc tccctacacg agcgccccgc cgctgtccca cacccccaat
     1441 gccgacttcc agcccccata cttcccccca ccctaccagc ctatctaccc ccagtcgcaa
     1501 gatccttact cccacgtcaa cgacccctac agcctgaacc ccctgcacgc ccagccgcag
     1561 ccgcagcacc caggctggcc cggccagagg cagagccagg agtctgggct cctgcacacg
     1621 caccgggggc tgcctcacca gctgtcgggc ctggatcctc gcagggacta caggcggcac
     1681 gaggacctcc tgcacggccc acacgcgctc agctcaggac tcggagacct ctcgatccac
     1741 tccttacctc acgccatcga ggaggtcccg catgtagaag acccgggtat taacatccca
     1801 gatcaaactg taattaagaa aggccccgtg tccctgtcca agtccaacag caatgccgtc
     1861 tccgccatcc ctattaacaa ggacaacctc ttcggcggcg tggtgaaccc caacgaagtc
     1921 ttctgttcag ttccgggtcg cctctcgctc ctcagctcca cctcgaagta caaggtcacg
     1981 gtggcggaag tgcagcggcg gctctcacca cccgagtgtc tcaacgcgtc gctgctgggc
     2041 ggagtgctcc ggagggcgaa gtctaaaaat ggaggaagat ctttaagaga aaaactggac
     2101 aaaataggat taaatctgcc tgcagggaga cgtaaagctg ccaacgttac cctgctcaca
     2161 tcactagtag agggagaagc tgtccaccta gccagggact ttgggtacgt gtgcgaaacc
     2221 gaatttcctg ccaaagcagt agctgaattt ctcaaccgac aacattccga tcccaatgag
     2281 caagtgacaa gaaaaaacat gctcctggct acaaaacaga tatgcaaaga gttcaccgac
     2341 ctgctggctc aggaccgatc tcccctgggg aactcacggc ccaaccccat cctggagccc
     2401 ggcatccaga gctgcttgac ccacttcaac ctcatctccc acggcttcgg cagccccgcg
     2461 gtgtgtgccg cggtcacggc cctgcagaac tatctcaccg aggccctcaa ggccatggac
     2521 aaaatgtacc tcagcaacaa ccccaacagc cacacggaca acaacgccaa aagcagtgac
     2581 aaagaggaga agcacagaaa gtagtaatga actagtccgt aacttgaaag tatttcgatt
     2641 tcttggcttt atatatcttg tggaaaggac gatcgatgcc atgttgtctc ggatacacgc
     2701 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2761 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2821 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2881 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2941 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3001 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3061 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3121 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3181 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3241 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3301 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3361 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     3421 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     3481 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     3541 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3601 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     3661 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     3721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3781 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     3841 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3901 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3961 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     4021 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4081 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4141 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     4201 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4261 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4321 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4381 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4441 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4501 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4561 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4621 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4681 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4741 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4801 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4861 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4921 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4981 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5041 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5101 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5161 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5221 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5281 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5341 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5401 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5461 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5521 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5581 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5641 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5701 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5761 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5821 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5881 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5941 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6001 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6061 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6421 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6481 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6541 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6601 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6661 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6721 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6781 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     6841 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     6901 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     6961 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     7021 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     7081 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7141 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7201 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7261 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     7321 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     7381 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     7441 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     7501 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     7561 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7621 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     7681 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     7741 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     7801 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7861 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7921 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7981 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     8041 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     8101 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     8161 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     8221 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     8281 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     8341 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     8401 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     8461 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     8521 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat gcattgcatc
     8581 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8641 aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     8701 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8761 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8821 ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct taccatgacc
     8881 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     8941 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     9001 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     9061 aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc
     9121 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     9181 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     9241 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     9301 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     9361 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     9421 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cagttaacga
     9481 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     9541 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     9601 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     9661 agatccactt tggcgccgg
//
