LOCUS       sequence-423582-       10584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian continuous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10584)
  TITLE     Yoshimatsu Lab plasmids 2023
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CSII-CAG-EGFP
FEATURES             Location/Qualifiers
     source          1..10584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..640
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(105..125)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(523..534)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1159..1339
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1365..1382)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1371..1595
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1641..2069
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1728..1747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1938..1959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2078..2098)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2083..2412
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2263..2398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2325..2344
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2460..2507
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2526..2900
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3030..3151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3067..3086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3121..3140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3200..3216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3200..3216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3213..3235)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3224..3240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3248..3254,3255..3272,3273..3278))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3293..3314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3431..3448)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3602..4190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3682..3701)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4361..5152,5153..5221))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4984..5003
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5222..5326)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5401..5420)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5592..5971
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5973..6171
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6126..6146
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6189..6369
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6416..6541
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7033..7266
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7451..7495
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7769..7886
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8033..8057
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        8070..8449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          8711..9727
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     9651..9673
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     9735..9754
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    9788..9808
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    9815..9839
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9857..9859,9860..9862,9863..10576)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9857..9859,9860..9862,9863..10573)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9902..9923)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10163..10182)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10510..10531
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(join(10574..10584,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatgct accggaacat cgagggatca agcttatcga taatcaacct
       61 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
      121 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
      181 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
      241 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
      301 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
      361 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
      421 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
      481 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
      541 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
      601 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgagaccta
      661 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
      721 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
      781 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
      841 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
      901 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
      961 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     1021 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     1081 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     1141 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     1201 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     1261 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     1321 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     1381 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     1441 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     1501 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     1561 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     1621 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     1681 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     1741 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     1801 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     1861 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     1921 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     1981 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2041 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     2101 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2161 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2221 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2281 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2341 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2401 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     2461 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     2521 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     2581 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     2641 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     2701 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     2761 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     2821 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     2881 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     2941 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     3001 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     3061 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3121 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     3181 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3241 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3301 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3361 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3421 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3481 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3541 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3601 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3661 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3721 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3781 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3841 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3901 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3961 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4021 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4081 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4141 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4201 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4261 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4321 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4381 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4441 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4501 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4561 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4621 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4681 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4741 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4801 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4861 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4921 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4981 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5041 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5101 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5161 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5221 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5281 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5341 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     5401 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     5461 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     5521 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     5581 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     5641 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     5701 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     5761 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     5821 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     5881 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     5941 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     6001 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     6061 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     6121 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     6181 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6241 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6301 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6361 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     6421 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     6481 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     6541 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     6601 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     6661 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     6721 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     6781 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     6841 ggaagagcaa aacaaaagta agaccaccgc acagcaaggg ccgctgatct tcagacctgg
     6901 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     6961 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     7021 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     7081 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     7141 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     7201 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     7261 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     7321 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     7381 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     7441 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     7501 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     7561 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     7621 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     7681 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     7741 atctacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     7801 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     7861 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga aattcacttt
     7921 gaattaattc aagcttcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc
     7981 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggctagca tcacaagttt
     8041 gtacaaaaaa gcagaagctt ccatgggtcg acattgatta ttgactagtt attaatagta
     8101 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8161 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     8221 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     8281 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     8341 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     8401 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     8461 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     8521 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg gggggggggg
     8581 cggggggcgg gggggggggg gggggagggg tgcgggggcg gccatgcaga gcggcgcgct
     8641 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     8701 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     8761 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     8821 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     8881 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     8941 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     9001 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     9061 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     9121 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     9181 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     9241 tacggggcgt ggcgcggggc tcgccgtacc gggcgggggg tggcggcagg tgggggtgcc
     9301 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     9361 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     9421 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     9481 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     9541 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     9601 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     9661 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     9721 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     9781 caagcttcaa ctttgtataa taaagttgga agcttcaagt ttgtacaaaa aagcaggctt
     9841 cgaaggagat agaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     9901 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     9961 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
    10021 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
    10081 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
    10141 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
    10201 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
    10261 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
    10321 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
    10381 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
    10441 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
    10501 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
    10561 cgagctgtac aagtaacagc tttc
//
