LOCUS       sequence-42361-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TDRD5
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             426..443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(496..514)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      510..557
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(681..700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctctgaata ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac
       61 agcagcggca gagaaaactt gtatttccag ggcatgtctg aacaagagcg tatacaggaa
      121 tgtctgcgga aggaaataag gtcacttctc atttccacca aagatggttt gagcccacag
      181 gagttggaga aggagtacct tttgatggtt ggcaaccatc taccactccg aatccttggg
      241 tatcggtcca ctatggagct ggtattggac atgcctgatg ttgttcgtgt ctgccccggt
      301 gcaggtggta ctgtaatact gaaagccatt ccagatgaat ctaccaaagg aatagcaagc
      361 ttagttgcaa aacagaggag cagccataag cttcgatgat gacgaagctt gcggccgcac
      421 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      481 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      541 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      661 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      721 cggctttccc cgtcaagctc taaatc
//
