LOCUS       sequence-423636-        9963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged mutant version of EZH1 partially resistant to 
            JQ-EZ-05 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Chakraborty AA, Nakamura E, Qi J, Creech A, Jaffe JD, Paulk J, Novak
            JS, Nagulapalli K, McBrayer SK, Cowley GS, Pineda J, Song J, Wang 
            YE, Carr SA, Root DE, Signoretti S, Bradner JE, Kaelin WG Jr
  TITLE     HIF activation causes synthetic lethality between the VHL tumor 
            suppressor and the EZH1 histone methyltransferase.
  JOURNAL   Sci Transl Med. 2017 Jul 12;9(398):eaal5272. doi: 
            10.1126/scitranslmed.aal5272.
  PUBMED    28701475
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2017-07-12"; volume: "9(398):eaal5272. doi"; pages: " 
            10.1126/scitranslmed.aal5272"
COMMENT     Sequence Label: pLX304-EZH1-Y727F
FEATURES             Location/Qualifiers
     source          1..9963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2862..2886)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2888..2929
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2971..3559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3024..3044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3442..3453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3631..3864
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3942..4063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3979..3998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4033..4052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4103..4238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4258..4277)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4259..4277)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4287..4304)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4287..4303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4296..4318)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4445..4900
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4532..4551)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4742..4763
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4926..5030
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5031..5099,5100..5891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5249..5268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6062..6650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6551..6570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6804..6821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6938..6959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6974..6979,6980..6997,6998..7004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7012..7028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7017..7039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7036..7052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7036..7052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7071..7091
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7073..7091
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7119..7345
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7346..7526
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7573..7698
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8191..8424
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8609..8653
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8828..9328
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9247..9265
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9340..9735
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9795..9912
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagttggca tggaaatacc aaatccccct acctccaaat gtatcactta
      661 ctggaaaaga aaagtgaaat ctgaatacat gcgacttcga caacttaaac ggcttcaggc
      721 aaatatgggt gcaaaggctt tgtatgtggc aaattttgca aaggttcaag aaaaaaccca
      781 gatcctcaat gaagaatgga agaagcttcg tgtccaacct gttcagtcaa tgaagcctgt
      841 gagtggacac ccttttctca aaaagtgtac catagagagc attttcccgg gatttgcaag
      901 ccaacatatg ttaatgaggt cactgaacac agttgcattg gttcccatca tgtattcctg
      961 gtcccctctc caacagaact ttatggtaga agatgagacg gttttgtgca atattcccta
     1021 catgggagat gaagtgaaag aagaagatga gacttttatt gaggagctga tcaataacta
     1081 tgatgggaaa gtccatggtg aagaagagat gatccctgga tccgttctga ttagtgatgc
     1141 tgtttttctg gagttggtcg atgccctgaa tcagtactca gatgaggagg aggaagggca
     1201 caatgacacc tcagatggaa agcaggatga cagcaaagaa gatctgccag taacaagaaa
     1261 gagaaagcga catgctattg aaggcaacaa aaagagttcc aagaaacagt tcccaaatga
     1321 catgatcttc agtgcaattg cctcaatgtt ccctgagaat ggtgtcccag atgacatgaa
     1381 ggagaggtat cgagaactaa cagagatgtc agaccccaat gcacttcccc ctcagtgcac
     1441 acccaacatc gatggcccca atgccaagtc tgtgcagcgg gagcaatctc tgcactcctt
     1501 ccacacactt ttttgccggc gctgctttaa atacgactgc ttccttcacc cttttcatgc
     1561 cacccctaat gtatataaac gcaagaataa agaaatcaag attgaaccag aaccatgtgg
     1621 cacagactgc ttccttttgc tggaaggagc aaaggagtat gccatgctcc acaacccccg
     1681 ctccaagtgc tctggtcgtc gccggagaag gcaccacata gtcagtgctt cctgctccaa
     1741 tgcctcagcc tctgctgtgg ctgagactaa agaaggagac agtgacaggg acacaggcaa
     1801 tgactgggcc tccagttctt cagaggctaa ctctcgctgt cagactccca caaaacagaa
     1861 ggctagtcca gccccacctc aactctgcgt agtggaagca ccctcggagc ctgtggaatg
     1921 gactggggct gaagaatctc tttttcgagt cttccatggc acctacttca acaacttctg
     1981 ttcaatagcc aggcttctgg ggaccaagac gtgcaagcag gtctttcagt ttgcagtcaa
     2041 agaatcactt atcctgaagc tgccaacaga tgagctcatg aacccctcac agaagaagaa
     2101 aagaaagcac agattgtggg ctgcacactg caggaagatt cagctgaaga aagataactc
     2161 ttccacacaa gtgtacaact accaaccctg cgaccaccca gaccgcccct gtgacagcac
     2221 ctgcccctgc atcatgactc agaatttctg tgagaagttc tgccagtgca acccagactg
     2281 tcagaatcgt ttccctggct gtcgctgtaa gacccagtgc aataccaagc aatgtccttg
     2341 ctatctggca gtgcgagaat gtgaccctga cctgtgtctc acctgtgggg cctcagagca
     2401 ctgggactgc aaggtggttt cctgtaaaaa ctgcagcatc cagcgtggac ttaagaagca
     2461 cctgctgctg gccccctctg atgtggccgg atggggcacc ttcataaagg agtctgtgca
     2521 gaagaacgaa ttcatttctg aatactgtgg tgagctcatc tctcaggatg aggctgatcg
     2581 acgcggaaag gtctatgaca aatacatgtc cagcttcctc ttcaacctca ataatgattt
     2641 tgtagtggat gctactcgga aaggaaacaa aattcgattt gcaaatcatt cagtgaatcc
     2701 caactgttat gccaaagtgg tcatggtgaa tggagaccat cggattggga tctttgccaa
     2761 gagggcaatt caagctggcg aagagctctt ctttgatttc aggtacagcc aagctgatgc
     2821 tctcaagtac gtggggatcg agagggagac cgacgtcctt tgcccaactt tcttgtacaa
     2881 agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc
     2941 tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     3001 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3061 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3121 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3181 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3241 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3301 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3361 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3421 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3481 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3541 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3601 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3661 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3721 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3781 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3841 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     3901 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     4081 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4141 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4201 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4261 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4321 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4381 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4441 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4501 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4561 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4621 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4681 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4741 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4801 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4861 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4921 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4981 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5041 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5101 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5161 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5221 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5281 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5341 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5401 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5461 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5521 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5581 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5641 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5701 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5761 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5821 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5881 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5941 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6001 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6061 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6121 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6181 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6241 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6301 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6361 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6421 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6481 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6541 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6601 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6661 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6721 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6781 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6841 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6901 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6961 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7021 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     7081 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     7141 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7201 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7261 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7321 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7381 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7441 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7501 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7561 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7621 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7681 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7741 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7801 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7861 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7921 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7981 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8041 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8101 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8161 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8221 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8281 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8341 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8401 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8461 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8521 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8581 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8641 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8701 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8761 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8821 gggacccttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     8881 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     8941 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     9001 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     9061 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     9121 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     9181 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     9241 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     9301 tgaccgaatc accgacctct ctccccaggg ggtaccacca tggccaagcc tttgtctcaa
     9361 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     9421 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     9481 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     9541 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     9601 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     9661 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     9721 tatgtgtggg agggcctgca gctgcagtag taagaattct agatcttgag acaaatggca
     9781 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     9841 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     9901 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag
     9961 ggg
//
