LOCUS       sequence-42364-e         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Weinman EJ, Steplock D, Cha B, Kovbasnjuk O, Frost NA, Cunningham R,
            Shenolikar S, Blanpied TA, Donowitz M
  TITLE     PTH transiently increases the percent mobile fraction of Npt2a in OK
            cells as determined by FRAP.
  JOURNAL   Am J Physiol Renal Physiol. 2009 Dec;297(6):F1560-5. Epub 2009 Sep 
            30.
  PUBMED    19794105
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Renal Physiol."; date: "2009-12"; volume: "297(6)"; pages: 
            "F1560-5. Epub 2009 Sep 30"
COMMENT     Sequence Label: pcDNA6-rbNHERF1
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             133..165
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(169..177,178..192)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
       61 ggagacccaa gctggctagc gtttaaactt aagcttacca tggggggttc tcatcatcat
      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      181 gatgacgata aggtaccagg atccagtgtg gtggaattcg ccgccatgag cgcggacgcg
      241 gcggccgggg cgcccctgcc ccggctctgc tgcctggaga aggggccgaa cggctacggc
      301 ttccacctgc atggggagaa gggcaaggtg ggccagtaca tccggctggt ggagcccggc
      361 tctccggccg agaaggcggg gctgctggcc ggggaccggc tggtggaggt gaacggcgag
      421 aacgtggaga aggagaccca ccagcaggtg gtgagccgca tccgcgccgc gctcaacgcc
      481 gtgcgcctgc tggtggtcga ccccgacacg gacgagcagt tcaggaagct cggagtccag
      541 atccgggggg agctgctacg cgcccaggcg gggccggagc aggccgggcc gccggccgcc
      601 cccggcgagc agggcccggc cggtgaga
//
