LOCUS       sequence-423655-       12553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in mammallian cells or oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Abderemane-Ali F, Findeisen F, Rossen ND, Minor DL Jr
  TITLE     A Selectivity Filter Gate Controls Voltage-Gated Calcium Channel 
            Calcium-Dependent Inactivation.
  JOURNAL   Neuron. 2019 Mar 20;101(6):1134-1149.e3. doi: 
            10.1016/j.neuron.2019.01.011. Epub 2019 Feb 4.
  PUBMED    30733149
REFERENCE   2  (bases 1 to 12553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.01.011"; journalName: "Neuron"; date: 
            "2019-03-20- 20"; volume: "101"; issue: "6"; pages: "1134-1149.e3"
COMMENT     Sequence Label: CaV2.1/pcDNA3.1 ~ Human
FEATURES             Location/Qualifiers
     source          1..12553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             938..961
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7595..7624
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(8144..8161)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8150..8374
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8420..8848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8507..8526)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8717..8738
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8857..8877)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8862..9191
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9042..9177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9104..9123
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9258..10052
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9312..9331)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9922..9941
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10226..10347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10263..10282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10317..10336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10396..10412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10396..10412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10409..10431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10420..10436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10444..10450,10451..10468,10469..10474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10489..10510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10627..10644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10798..11386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10878..10897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11557..12348,12349..12417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12180..12199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12418..12522)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatggac tacaaagacg atgacgacaa
      961 gcttatggcc cgcttcggag acgagatgcc ggcccgctac gggggaggag gctccggggc
     1021 agccgccggg gtggtcgtgg gcagcggagg cgggcgagga gccgggggca gccggcaggg
     1081 cgggcagccc ggggcgcaaa ggatgtacaa gcagtcaatg gcgcagagag cgcggaccat
     1141 ggcactctac aaccccatcc ccgtccgaca gaactgcctc acggttaacc ggtctctctt
     1201 cctcttcagc gaagacaacg tggtgagaaa atacgccaaa aagatcaccg aatggcctcc
     1261 ctttgaatat atgattttag ccaccatcat agcgaattgc atcgtcctcg cactggagca
     1321 gcatctgcct gatgatgaca agaccccgat gtctgaacgg ctggatgaca cagaaccata
     1381 cttcattgga attttttgtt tcgaggctgg aattaaaatc attgcccttg ggtttgcctt
     1441 ccacaaaggc tcctacttga ggaatggctg gaatgtcatg gactttgtgg tggtgctaac
     1501 gggcatcttg gcgacagttg ggacggagtt tgacctacgg acgctgaggg cagttcgagt
     1561 gctgcggccg ctcaagctgg tgtctggaat cccaagttta caagtcgtcc tgaagtcgat
     1621 catgaaggcg atgatccctt tgctgcagat cggcctcctc ctattttttg caatccttat
     1681 ttttgcaatc atagggttag aattttatat gggaaaattt cataccacct gctttgaaga
     1741 ggggacagat gacattcagg gtgagtctcc ggctccatgt gggacagaag agcccgcccg
     1801 cacctgcccc aatgggacca aatgtcagcc ctactgggaa gggcccaaca acgggatcac
     1861 tcagttcgac aacatcctgt ttgcagtgct gactgttttc cagtgcataa ccatggaagg
     1921 gtggactgat ctcctctaca atagcaacga tgcctcaggg aacacttgga actggttgta
     1981 cttcatcccc ctcatcatca tcggctcctt ttttatgctg aaccttgtgc tgggtgtgct
     2041 gtcaggggag tttgccaaag aaagggaacg ggtggagaac cggcgggctt ttctgaagct
     2101 gaggcggcaa caacagattg aacgtgagct caatgggtac atggaatgga tctcaaaagc
     2161 agaagaggtg atcctcgccg aggatgaaac tgacggggag cagaggcatc cctttgatgg
     2221 agctctgcgg agaaccacca taaagaaaag caagacagat ttgctcaacc ccgaagaggc
     2281 tgaggatcag ctggctgata tagcctctgt gggttctccc ttcgcccgag ccagcattaa
     2341 aagtgccaag ctggagaact cgaccttttt tcacaaaaag gagaggagga tgcgtttcta
     2401 catccgccgc atggtcaaaa ctcaggcctt ctactggact gtactcagtt tggtagctct
     2461 caacacgctg tgtgttgcta ttgttcacta caaccagccc gagtggctct ccgacttcct
     2521 ttactatgca gaattcattt tcttaggact ctttatgtcc gaaatgttta taaaaatgta
     2581 cgggcttggg acgcggcctt acttccactc ttccttcaac tgctttgact gtggggttat
     2641 cattgggagc atcttcgagg tcatctgggc tgtcataaaa cctggcacat cctttggaat
     2701 cagcgtgtta cgagccctca ggttattgcg tattttcaaa gtcacaaagt actgggcatc
     2761 tctcagaaac ctggtcgtct ctctcctcaa ctccatgaag tccatcatca gcctgttgtt
     2821 tctccttttc ctgttcattg tcgtcttcgc ccttttggga atgcaactct tcggcggcca
     2881 gtttaatttc gatgaaggga ctcctcccac caacttcgat acttttccag cagcaataat
     2941 gacggtgttt cagatcctga cgggcgaaga ctggaacgag gtcatgtacg acgggatcaa
     3001 gtctcagggg ggcgtgcagg gcggcatggt gttctccatc tatttcattg tactgacgct
     3061 ctttgggaac tacaccctcc tgaatgtgtt cttggccatc gctgtggaca atctggccaa
     3121 cgcccaggag ctcaccaagg acgagcaaga ggaagaagaa gcagcgaacc agaaacttgc
     3181 cctacagaaa gccaaggagg tggcagaagt gagtcctctg tccgcggcca acatgtctat
     3241 agctgtgaaa gagcaacaga agaatcaaaa gccagccaag tccgtgtggg agcagcggac
     3301 cagtgagatg cgaaagcaga acttgctggc cagccgggag gccctgtata acgaaatgga
     3361 cccggacgag cgctggaagg ctgcctacac gcggcacctg cggccagaca tgaagacgca
     3421 cttggaccgg ccgctggtgg tggacccgca ggagaaccgc aacaacaaca ccaacaagag
     3481 ccgggcggcc gagcccaccg tggaccagcg cctcggccag cagcgcgccg aggacttcct
     3541 caggaaacag gcccgctacc acgatcgggc ccgggacccc agcggctcgg cgggcctgga
     3601 cgcacggagg ccctgggcgg gaagccagga ggccgagctg agccgggagg gaccctacgg
     3661 ccgcgagtcg gaccaccacg cccgggaggg cagcctggag caacccgggt tctgggaggg
     3721 cgaggccgag cgaggcaagg ccggggaccc ccaccggagg cacgtgcacc ggcagggggg
     3781 cagcagggag agccgcagcg ggtccccgcg cacgggcgcg gacggggagc atcgacgtca
     3841 tcgcgcgcac cgcaggcccg gggaggaggg tccggaggac aaggcggagc ggagggcgcg
     3901 gcaccgcgag ggcagccggc cggcccgggg cggcgagggc gagggcgagg gccccgacgg
     3961 gggcgagcgc aggagaaggc accggcatgg cgctccagcc acgtacgagg gggacgcgcg
     4021 gagggaggac aaggagcgga ggcatcggag gaggaaagag aaccagggct ccggggtccc
     4081 tgtgtcgggc cccaacctgt caaccacccg gccaatccag caggacctgg gccgccaaga
     4141 cccacccctg gcagaggata ttgacaacat gaagaacaac aagctggcca ccgcggagtc
     4201 ggccgctccc cacggcagcc ttggccacgc cggcctgccc cagagcccag ccaagatggg
     4261 aaacagcacc gaccccggcc ccatgctggc catccctgcc atggccacca acccccagaa
     4321 cgccgccagc cgccggacgc ccaacaaccc ggggaaccca tccaatcccg gcccccccaa
     4381 gacccccgag aatagcctta tcgtcaccaa ccccagcggc acccagacca attcagctaa
     4441 gactgccagg aaacccgacc acaccacagt ggacatcccc ccagcctgcc caccccccct
     4501 caaccacacc gtcgtacaag tgaacaaaaa cgccaaccca gacccactgc caaaaaaaga
     4561 ggaagagaag aaggaggagg aggaagacga ccgtggggaa gacggcccta agccaatgcc
     4621 tccctatagc tccatgttca tcctgtccac gaccaacccc cttcgccgcc tgtgccatta
     4681 catcctgaac ctgcgctact ttgagatgtg catcctcatg gtcattgcca tgagcagcat
     4741 cgccctggcc gccgaggacc ctgtgcagcc caacgcacct cggaacaacg tgctgcgata
     4801 ctttgactac gtttttacag gcgtctttac ctttgagatg gtgatcaaga tgattgacct
     4861 ggggctcgtc ctgcatcagg gtgcctactt ccgtgacctc tggaatattc tcgacttcat
     4921 agtggtcagt ggggccctgg tagcctttgc cttcactggc aatagcaaag gaaaagacat
     4981 caacacgatt aaatccctcc gagtcctccg ggtgctacga cctcttaaaa ccatcaagcg
     5041 gctgccaaag ctcaaggctg tgtttgactg tgtggtgaac tcacttaaaa acgtcttcaa
     5101 catcctcatc gtctacatgc tattcatgtt catcttcgcc gtggtggctg tgcagctctt
     5161 caaggggaaa ttcttccact gcactgacga gtccaaagag tttgagaaag attgtcgagg
     5221 caaatacctc ctctacgaga agaatgaggt gaaggcgcga gaccgggagt ggaagaagta
     5281 tgaattccat tacgacaatg tgctgtgggc tctgctgacc ctcttcaccg tgtccacggg
     5341 agaaggctgg ccacaggtcc tcaagcattc ggtggacgcc acctttgaga accagggccc
     5401 cagccccggg taccgcatgg agatgtccat tttctacgtc gtctactttg tggtgttccc
     5461 cttcttcttt gtcaatatct ttgtggcctt gatcatcatc accttccagg agcaagggga
     5521 caagatgatg gaggaataca gcctggagaa aaatgagagg gcctgcattg atttcgccat
     5581 cagcgccaag ccgctgaccc gacacatgcc gcagaacaag cagagcttcc agtaccgcat
     5641 gtggcagttc gtggtgtctc cgcctttcga gtacacgatc atggccatga tcgccctcaa
     5701 caccatcgtg cttatgatga agttctatgg ggcttctgtt gcttatgaaa atgccctgcg
     5761 ggtgttcaac atcgtcttca cctccctctt ctctctggaa tgtgtgctga aagtcatggc
     5821 ttttgggatt ctgaattatt tccgcgatgc ctggaacatc ttcgactttg tgactgttct
     5881 gggcagcatc accgatatcc tcgtgactga gtttgggaat aacttcatca acctgagctt
     5941 tctccgcctc ttccgagctg cccggctcat caaacttctc cgtcagggtt acaccatccg
     6001 cattcttctc tggacctttg tgcagtcctt caaggccctg ccttatgtct gtctgctgat
     6061 cgccatgctc ttcttcatct atgccatcat tgggatgcag gtgtttggta acattggcat
     6121 cgacgtggag gacgaggaca gtgatgaaga tgagttccaa atcactgagc acaataactt
     6181 ccggaccttc ttccaggccc tcatgcttct cttccggagt gccaccgggg aagcttggca
     6241 caacatcatg ctttcctgcc tcagcgggaa accgtgtgat aagaactctg gcatcctgac
     6301 tcgagagtgt ggcaatgaat ttgcttattt ttactttgtt tccttcatct tcctctgctc
     6361 gtttctgatg ctgaatctct ttgtcgccgt catcatggac aactttgagt acctcacccg
     6421 agactcctcc atcctgggcc cccaccacct ggatgagtac gtgcgtgtct gggccgagta
     6481 tgaccccgca gcttgcggtc ggattcatta taaggatatg tacagtttat tacgagtaat
     6541 atctccccct ctcggcttag gcaagaaatg tcctcatagg gttgcttgca agcggcttct
     6601 gcggatggac ctgcccgtcg cagatgacaa caccgtccac ttcaattcca ccctcatggc
     6661 tctgatccgc acagccctgg acatcaagat tgccaaggga ggagccgaca aacagcagat
     6721 ggacgctgag ctgcggaagg agatgatggc gatttggccc aatctgtccc agaagacgct
     6781 agacctgctg gtcacacctc acaagtccac ggacctcacc gtggggaaga tctacgcagc
     6841 catgatgatc atggagtact accggcagag caaggccaag aagctgcagg ccatgcgcga
     6901 ggagcaggac cggacacccc tcatgttcca gcgcatggag cccccgtccc caacgcagga
     6961 agggggacct ggccagaacg ccctcccctc cacccagctg gacccaggag gagccctgat
     7021 ggctcacgaa agcggcctca aggagagccc gtcctgggtg acccagcgtg cccaggagat
     7081 gttccagaag acgggcacat ggagtccgga acaaggcccc cctaccgaca tgcccaacag
     7141 ccagcctaac tctcagtccg tggagatgcg agagatgggc agagatggct actccgacag
     7201 cgagcactac ctccccatgg aaggccaggg ccgggctgcc tccatgcccc gcctccctgc
     7261 agagaaccag aggagaaggg gccggccacg tgggaataac ctcagtacca tctcagacac
     7321 cagccccatg aagcgttcag cctccgtgct gggccccaag gcccgacgcc tggacgatta
     7381 ctcgctggag cgggtcccgc ccgaggagaa ccagcggcac caccagcggc gccgcgaccg
     7441 cagccaccgc gcctctgagc gctccctggg ccgctacacc gatgtggaca caggcttggg
     7501 gacagacctg agcatgacca cccaatccgg ggacctgccg tcgaaggagc gggaccagga
     7561 gcggggccgg cccaaggatc ggaagcatcg acagcaccac caccaccacc accaccacca
     7621 ccaccccccg ccccccgaca aggaccgcta tgcccaggaa cggccggacc acggccgggc
     7681 acgggctcgg gaccagcgct ggtcccgctc gcccagcgag ggccgagagc acatggcgca
     7741 ccggcagtag ttccgtaagt ggaagcccag ccccctcaac atctggtacc agcactccgc
     7801 ggcggggccg ccgccagctc ccccagaccc cctccacccc ccggccacac gtgtcctatt
     7861 cccctgtgat ccgtaaggcc ggcggctcgg ggcccccgca gcagcagcag cagcagcagc
     7921 agcagcagca gcaggcggtg gccaggccgg gccgggcggc caccagcggc cctcggaggt
     7981 acccaggccc cacggccgag cctctggccg gagatcggcc gcccacgggg ggccacagca
     8041 gcggccgctc gcccaggatg gagaggcggg tcccaggccc ggcccggagc gagtccccca
     8101 gggcctgtcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     8161 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8221 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     8281 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8341 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     8401 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8461 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8521 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8581 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8641 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8701 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8761 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8821 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     8881 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8941 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9001 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     9061 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9121 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9181 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     9241 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     9301 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     9361 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     9421 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     9481 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     9541 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     9601 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     9661 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     9721 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     9781 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     9841 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     9901 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     9961 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
    10021 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
    10081 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
    10141 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
    10201 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
    10261 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10321 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
    10381 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10441 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10501 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10561 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10621 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10681 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10741 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    10981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11221 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11281 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11521 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11581 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11641 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11761 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11821 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11881 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    11941 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12061 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12121 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12181 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12241 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12361 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12421 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12481 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12541 gccacctgac gtc
//
