LOCUS       sequence-42369-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PANK3
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccctctgaat attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggttccgc gtggtagttc tttcccatgg tttggcatgg
      121 acattggggg aactctagta aagctctcgt actttgaacc tattgatatc acagcagagg
      181 aagagcaaga agaagttgag agtttaaaaa gtattcggaa atatttgact tctaacgtgg
      241 catatggatc caccggcatt cgggatgtac accttgaact gaaagattta acactttttg
      301 gccgaagagg gaacttgcac tttatcaggt ttccaaccca ggacctgcct acttttatcc
      361 aaatgggaag agataaaaac ttctcaacat tgcagacggt gctatgtgct acaggaggtg
      421 gtgcttacaa gtttgaaaaa gattttcgca caattggaaa cctccacctg cacaaactgg
      481 atgaacttga ctgccttgta aagggcttgc tgtatataga ctctgtcagt ttcaatggac
      541 aagccgagtg ctattatttt gctaatgcct cagaacctga gcgatgccaa aagatgcctt
      601 ttaacctgga tgatccctat ccactgcttg tagtgaacat tggctcagga gtcagtattt
      661 tagcagtcca ttccaaagac aactataaac gagtgactgg gacaagcctt ggagggggta
      721 cctttctggg tttatgcagt ttattgactg gctgtgaaag ttttgaagaa gctcttg
//
