LOCUS       sequence-42372-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00004
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(435..534)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(551..570)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(552..570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(575..591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(575..591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(693..712)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ctgatagtga cctgtttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac
       61 tttgtacaaa aaagttggca ccatggctga tatggagata cagagcaaca aaatgtcaat
      121 aacggaggaa acacaagtga cgcgcaagga atgtggaaaa aggggacgaa aaccaggaag
      181 aaagacgtct actgaaaaat tggacatgaa agccaaacta gaacgcagca gacaaagtgc
      241 cagggaatgc cgggcgcgca agaagctgcg gtatcagtac ctggaggaac tggtggcgga
      301 tcgggagaag gctgtagttg ctttgcgtac ggaactggag cgcttaattc aatggaataa
      361 ccagttgagc gaaagcaaca ctccaaccaa caatgaccag ttacttcagg aactcggaat
      421 cctcaaacaa gaattgccaa ctttcttgta caaagttggc attataagaa agcattgctt
      481 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
      541 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
      601 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
      661 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
      721 ggaaacgtcg aggccgcgat taaattccaa c
//
