LOCUS       sequence-42375-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00018
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(650..749)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(766..785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(767..785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(790..806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(790..806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
       61 ttgtacaaaa aagttggcac catggaacgg agtcatcttt acttgcccac tctgagctac
      121 gcggccatgg gtcacgtata cgcaccgtat cgcggaagca gttcacccgc actatcgaca
      181 gcatcgtcaa catcatcgaa gccggagcag atcgaggagc tggtgtccca gcagctgcat
      241 catctcaaaa tgcactacgc cgacgaggag caacgctacg tagaccagat gctcctggag
      301 aatcccattg ttgtggagcg ccgtgcaccg ccgccgctga aaacggagct tgctatggat
      361 tgccgcggat cgggttccgg atcaggttcc ggttctggtt cggatgtcaa ggatgcccag
      421 cgtcagaggg ccgagtcgtg ccgcaaatcc cgctacaaca acaagatcaa gaaggccaag
      481 ctgcgcttcc ggcacaaatt cgtcagcggg cagctgaaga agagcgccgt catgttggac
      541 acgatgcggg atgtgattgc ccaggcggag aggcagctgc tcgagcgggg atatcccgcc
      601 gccacactcg agcggatgcg ggccactttc ggcctggaaa tggagcagtt gccaactttc
      661 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
      721 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
      781 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
      841 tacattgcac aagataaaaa tatatcatca tgaacaataa
//
