LOCUS       sequence-42376-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00019
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(650..749)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(766..785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(767..785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(790..806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(790..806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
       61 ttgtacaaaa aagttggcac catgatgggc actatgtcgc tgcgccagcc agagaattgg
      121 ctatacgaga tttggcagaa gggctacgac agcccctgct atgtgatggt gcctaatgca
      181 aaagatctgg tcaagtcgca tctcatgata gcggtgcgcg aggaggtgga agtgctaaaa
      241 gagcgcatct ccgagctgat ggacaagatc aacaagctgg agctcgagaa cagcattctc
      301 aagtcgaaca tcccgcagga gacactgcag cagctgcagt tgcagctaca actcgccgca
      361 ccgccggcca cgcccgccat tcaagcggca ccggcggtac aaagtgtcgt tgctccagct
      421 gccgcgggtc aggcagttca gcagcaggcc gctggagcgg tagcggtaac gggtgtggcc
      481 accagtccag cgtccgcggt ggtacccacg agcattccca acggcagcgc cgagaacggc
      541 agcagtgcag tcgagtcagc agcggtatcg gtggagcagc aggtgcagca ggtgacatca
      601 gcagcggcag cagcagcatc ggtagtcaca gccaatggtc ccatgtcctt gccaactttc
      661 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
      721 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
      781 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
      841 tacattgcac aagataaaaa
//
