LOCUS       sequence-42377-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HECW2
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             485..502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(555..573)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      569..616
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(740..759)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccctctgaa tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcctgca gcgggccaac agcgacaccg
      121 acctggtgac ttctgagagc cgctccagct taactgccag catgtacgag tacacgctgg
      181 ggcaagccca gaacctcatt atcttctggg acattaaaga ggaggtggac cccagtgatt
      241 ggattggact ttatcatata gatgagaatt ctccagccaa cttctgggat tctaagaaca
      301 ggggtgtgac tggaacacaa aaagggcaaa ttgtatggag aattgagcct ggaccctatt
      361 tcatggaacc ggagataaaa atctgtttta aatattacca cggcattagt ggagccctgc
      421 gagccacgac cccctgcatc accgtgaaga acccatgatg acgaagcttg cggccgcact
      481 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      541 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      601 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      661 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      721 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      781 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      841 cggcac
//
