LOCUS       sequence-42384-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Weinman EJ, Steplock D, Cha B, Kovbasnjuk O, Frost NA, Cunningham R,
            Shenolikar S, Blanpied TA, Donowitz M
  TITLE     PTH transiently increases the percent mobile fraction of Npt2a in OK
            cells as determined by FRAP.
  JOURNAL   Am J Physiol Renal Physiol. 2009 Dec;297(6):F1560-5. Epub 2009 Sep 
            30.
  PUBMED    19794105
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Renal Physiol."; date: "2009-12"; volume: "297(6)"; pages: 
            "F1560-5. Epub 2009 Sep 30"
COMMENT     Sequence Label: pcDNA6-rbN1 S77A
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             124..141
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             145..177
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(181..189,190..204)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttac catggggggt
      121 tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg
      181 gatctgtacg acgatgacga taaggtacca ggatccagtg tggtggaatt cgccgccatg
      241 agcgcggacg cggcggccgg ggcgcccctg ccccggctct gctgcctgga gaaggggccg
      301 aacggctacg gcttccacct gcatggggag aagggcaagg tgggccagta catccggctg
      361 gtggagcccg gctctccggc cgagaaggcg gggctgctgg ccggggaccg gctggtggag
      421 gtgaacggcg agaacgtgga gaaggagacc caccagcagg tggtggcccg catccgcgcc
      481 gcgctcaacg ccgtgcgcct gctggtggtc gaccccgaca cggacgagca gttcaggaag
      541 ctcggagtcc agatccgggg ggagctgcta cgcgcccagg cg
//
