LOCUS       sequence-42386-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Weinman EJ, Steplock D, Cha B, Kovbasnjuk O, Frost NA, Cunningham R,
            Shenolikar S, Blanpied TA, Donowitz M
  TITLE     PTH transiently increases the percent mobile fraction of Npt2a in OK
            cells as determined by FRAP.
  JOURNAL   Am J Physiol Renal Physiol. 2009 Dec;297(6):F1560-5. Epub 2009 Sep 
            30.
  PUBMED    19794105
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Renal Physiol."; date: "2009-12"; volume: "297(6)"; pages: 
            "F1560-5. Epub 2009 Sep 30"
COMMENT     Sequence Label: pcDNA6-rbN1 S77D
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..120
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(124..132,133..147)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 acgactcact tatagggaga cccaagctgg ctagcgttta aacttaagct taccatgggg
       61 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
      121 cgggatctgt acgacgatga cgataaggta ccaggatcca gtgtggtgga attcgccgcc
      181 atgagcgcgg acgcggcggc cggggcgccc ctgccccggc tctgctgcct ggagaagggg
      241 ccgaacggct acggcttcca cctgcatggg gagaagggca aggtgggcca gtacatccgg
      301 ctggtggagc ccggctctcc ggccgagaag gcggggctgc tggccgggga ccggctggtg
      361 gaggtgaacg gcgagaacgt ggagaaggag acccatcagc aggtggtgga ccgcatccgc
      421 gccgcgctca acgccgtgcg cctgctggtg gtcgaccccg acacggacga gcagttcagg
      481 aagctcggag tccagatccg gggggagctg ctacgcgccc aggcggggcc ggagcaggcc
      541 gggccgccgg ccgcccccgg cgagcagggc ccggccggtg agaacgagcc gcgcgaggtc
      601 gagaagagcc acccggagcg gcgcgagctc cggcctcggc tctgcgccat gaagaagggc
      661 cccaacggct acggcttcaa cctgcacagc gacaagtcca ggccgggcca gttcatccgc
      721 gccgtggacc
//
