LOCUS       sequence-42390-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00415
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(412..511)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(528..547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(529..547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(552..568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(552..568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(670..689)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(748..767)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gatagtgacc tgtttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
       61 tgtacaaaaa agttggcacc atggaacaaa atgcgaagcc taaacgctcc tttaagcgtc
      121 cgtttacctt tccaaagaac tgtgtttacc gccctctacg tcagataagc aacatggagc
      181 gtagccagaa gctgtccgcc gaacaaccca cctacttcac gctcaatgca ccgccctcgt
      241 tggttccggc caagaagtac tcggatatca gcggtctgcc cgctccttat gccgatccgc
      301 acacgaaact aaggttcgcc agcgccgacg agtacgcgtc catgcagcac atgccctcgg
      361 acatcgtcaa tggctacctc atggtgcgcg gctataccag cgccgtggga ttgccaactt
      421 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
      481 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
      541 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
      601 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      661 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
      721 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      781 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
//
