LOCUS       sequence-423935-       13365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA 
            in mammilian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Klomp JE, Diehl JN, Klomp JA, Edwards AC, Yang R, Morales AJ, Taylor
            KE, Drizyte-Miller K, Bryant KL, Schaefer A, Johnson JL, Huntsman 
            EM, Yaron TM, Pierobon M, Baldelli E, Prevatte AW, Barker NK, 
            Herring LE, Petricoin EF 3rd, Graves LM, Cantley LC, Cox AD, Der CJ,
            Stalnecker CA
  TITLE     Determining the ERK-regulated phosphoproteome driving KRAS-mutant 
            cancer.
  JOURNAL   Science. 2024 Jun 7;384(6700):eadk0850. doi: 
            10.1126/science.adk0850. Epub 2024 Jun 7.
  PUBMED    38843329
REFERENCE   2  (bases 1 to 13365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-7"; volume: "384(6700):eadk0850. doi"; pages: " 
            10.1126/science.adk0850. Epub 2024 Jun 7"
COMMENT     Sequence Label: pInducer-myc-ERK1-R84S/D338N
FEATURES             Location/Qualifiers
     source          1..13365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2108,2109..2127,2128..2150,
                     2151..2169,2170..2192,2193..2211)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1983..2001,2002..2024,2025..2043,2044..2066,2067..2085,
                     2086..2108,2109..2127,2128..2150,2151..2169,2170..2192,
                     2193..2211,2212..2234,2235..2253)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2025..2043,2044..2066,2067..2085,2086..2108,2109..2127,
                     2128..2150,2151..2169,2170..2192,2193..2211,2212..2234,
                     2235..2253,2254..2276,2277..2295)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2328..2366
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2363..2387
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2459..2488
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3654..4865
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4702..4721
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4872..4905
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4912..5529,5530..5535,5536..5571,5572..5574,5575..5610,
                     5611..5613,5614..5649,5650..5658)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5707..6279
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5874..5891)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6101..6120
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6282..6884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6781..6801
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6906..7494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6959..6979)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7377..7388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7701..7934
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8035..8052)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8041..8265
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8311..8739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8398..8417)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8608..8629
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8748..8768)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8753..9082
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8933..9068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8995..9014
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9905..10026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10075..10091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10075..10091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10088..10110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10099..10115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10123..10129,10130..10147,10148..10153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10168..10189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10306..10323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10477..11065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10557..10576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11236..12027,12028..12096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11859..11878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12097..12201)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12249..12383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12551..13184
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13231..13356
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2281 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt aggcgtgtac
     2341 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     2401 atccacgctg ttttgacctc catagaagac accgggaccg atccagccta ccggtatgga
     2461 gcagaagctg atctccgagg aggacctggg atctgggcat atggcggcgg cggcggctca
     2521 ggggggcggg ggcggggagc cccgtagaac cgagggggtc ggcccggggg tcccggggga
     2581 ggtggagatg gtgaaggggc agccgttcga cgtgggcccg cgctacacgc agttgcagta
     2641 catcggcgag ggcgcgtacg gcatggtcag ctcggcctat gaccacgtgc gcaagactcg
     2701 cgtggccatc aagaagatca gccccttcga acatcagacc tactgccaga gcacgctccg
     2761 ggagatccag atcctgctgc gcttccgcca tgagaatgtc atcggcatcc gagacattct
     2821 gcgggcgtcc accctggaag ccatgagaga tgtctacatt gtgcaggacc tgatggagac
     2881 tgacctgtac aagttgctga aaagccagca gctgagcaat gaccatatct gctacttcct
     2941 ctaccagatc ctgcggggcc tcaagtacat ccactccgcc aacgtgctcc accgagatct
     3001 aaagccctcc aacctgctca tcaacaccac ctgcgacctt aagatttgtg atttcggcct
     3061 ggcccggatt gccgatcctg agcatgacca caccggcttc ctgacggagt atgtggctac
     3121 gcgctggtac cgggccccag agatcatgct gaactccaag ggctatacca agtccatcga
     3181 catctggtct gtgggctgca ttctggctga gatgctctct aaccggccca tcttccctgg
     3241 caagcactac ctggatcagc tcaaccacat tctgggcatc ctgggctccc catcccagga
     3301 ggacctgaat tgtatcatca acatgaaggc ccgaaactac ctacagtctc tgccctccaa
     3361 gaccaaggtg gcttgggcca agcttttccc caagtcagac tccaaagccc ttgacctgct
     3421 ggaccggatg ttaaccttta accccaataa acggatcaca gtggaggaag cgctggctca
     3481 cccctacctg gagcagtact atgacccgac gaatgagcca gtggccgagg agcccttcac
     3541 cttcgccatg gagctggatg acctacctaa ggagcggctg aaggagctca tcttccagga
     3601 gacagcacgc ttccagcccg gagtgctgga ggccccctag acgcgtaaga tctggcctcc
     3661 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
     3721 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
     3781 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
     3841 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
     3901 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
     3961 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
     4021 cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg
     4081 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
     4141 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa
     4201 atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt
     4261 gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat
     4321 gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga
     4381 agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg
     4441 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
     4501 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgtggtagg
     4561 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
     4621 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
     4681 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
     4741 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
     4801 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
     4861 tagacggatc cataacttcg tataatgtat gctatacgaa gttatgccac catgtctaga
     4921 ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt cggtatcgaa
     4981 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     5041 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     5101 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     5161 aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     5221 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     5281 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     5341 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     5401 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     5461 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     5521 gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat
     5581 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     5641 gacatgctcc ccgggtaaat aacttcgtat agcatacatt atacgaagta tggcgcgcca
     5701 attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     5761 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     5821 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     5881 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     5941 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     6001 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6061 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6121 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6181 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     6241 ccacggggac gtggttttcc tttgaaaaac acgataatac catggccacc gagtacaagc
     6301 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     6361 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     6421 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     6481 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6541 cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6601 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6661 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6721 ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt cctggagacc tccgcgcccc
     6781 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6841 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgattaatt aagcgcgtct
     6901 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6961 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7021 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7081 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7141 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7201 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7261 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7321 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7381 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7441 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7501 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7561 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7621 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7681 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatctgct
     7741 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7801 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7861 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7921 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7981 gaaatgaata tcagagagtg agaggccttg acattgttta aacccgctga tcagcctcga
     8041 ctgtgccttc tagttgccag ccatctgttg tttgcccctt ccccgtgcct tccttgaccc
     8101 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     8161 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     8221 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     8281 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     8341 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8401 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8461 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8521 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8581 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8641 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8701 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     8761 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8821 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8881 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8941 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9001 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     9061 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     9121 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtga ccgcaaggaa tcggtcaata
     9181 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     9241 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     9301 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     9361 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     9421 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     9481 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     9541 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     9601 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     9661 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     9721 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
     9781 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     9841 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     9901 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9961 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac taatcaatgt
    10021 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
    10081 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10141 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10201 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10261 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10321 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10381 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10441 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10501 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10561 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10621 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10681 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcactaacc
    10741 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10801 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10861 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10921 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10981 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11041 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11101 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11161 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11221 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11281 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11341 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11401 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11461 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11521 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11581 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11641 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11701 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11761 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11821 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11881 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11941 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12001 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12061 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12121 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12181 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    12241 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    12301 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    12361 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    12421 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    12481 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    12541 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
    12601 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
    12661 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
    12721 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
    12781 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
    12841 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
    12901 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
    12961 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
    13021 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    13081 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    13141 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
    13201 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
    13261 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    13321 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaag
//
