LOCUS       sequence-423963-        8529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses arabinose-inducible JUB1-derived artificial transcription 
            factor in bacterial cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Naseri G, Raasch H, Charpentier E, Erhardt M
  TITLE     A versatile regulatory toolkit of arabinose-inducible artificial 
            transcription factors for Enterobacteriaceae.
  JOURNAL   Commun Biol. 2023 Oct 3;6(1):1005. doi: 10.1038/s42003-023-05363-3.
  PUBMED    37789111
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-10-3"; volume: "6(1):1005. doi"; pages: " 
            10.1038/s42003-023-05363-3"
COMMENT     Sequence Label: pJUB1
FEATURES             Location/Qualifiers
     source          1..8529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      734..1322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1223..1242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2247..2461)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      3062..3939
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     4110..4127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(4285..5079)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHADSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4396..4415)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5006..5025
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5080..5184)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5321..5345
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     RBS             7844..7855
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(7885..8169)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(7943..7962)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             join(8260..8529,1..519)
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(8406..8425)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
       61 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      121 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      181 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      241 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      301 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
      361 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
      421 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
      481 atcgctgaga taggtgcctc actgattaag cattggtaaa attattaacg cttacaattt
      541 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca
      601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      661 tgtatccgct ggatctcatg ctggagttct tcgcccaccc ttaattaacc gtagaaaaga
      721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      841 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcatg
     1381 atataattaa attgaagctc taatttgtga gtttagtata catgcattta cttataatac
     1441 agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta
     1501 acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat
     1561 aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc
     1621 ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca
     1681 cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca
     1741 acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac
     1801 atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca
     1861 atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat
     1921 acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg
     1981 aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg
     2041 gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct
     2101 tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt
     2161 tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca
     2221 cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcggtt tttgttctgt
     2281 gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat
     2341 ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat
     2401 caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga
     2461 aaagctagct tatcgatgat aagctgtcaa agatgagaat taattccacg gactatagac
     2521 tatacctagt atactccgtc tactgtacga tacacttccg ctcaggtcct tgtcctttaa
     2581 cgaggcctta ccactctttt gttactctat tgatccagct cagcaaaggc agtgtgatct
     2641 aagattctat cttcgcgatg tagtaaaact agctagaccg agaaagagac tagaaatgca
     2701 aaaggcactt ctacaatggc tgccatcatt attatccgat gtgacgctgc agcttctcaa
     2761 tgatattcga atacgctttg aggagataca gcctaatatc cgacaaactg ttttacagat
     2821 ttacgatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc
     2881 tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga
     2941 caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca
     3001 cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt
     3061 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     3121 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     3181 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     3241 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     3301 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     3361 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     3421 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     3481 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     3541 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     3601 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     3661 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     3721 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     3781 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     3841 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     3901 atacttttga gcaatgtttg tggaagcggt attcgcaatg ggaagctcca ccccggttga
     3961 taatcagaaa agccccaaaa acaggaagat tgtataagca aatatttaaa ttgtaattct
     4021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagg
     4141 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4201 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4261 atatgagtaa acttggtctg acagtcagaa gaactcgtca agaaggcgat agaaggcgat
     4321 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     4381 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     4441 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     4501 caagcaggca tcgccatgag tcacgacgag atcctcgccg tcgggcatgc tcgccttgag
     4561 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     4621 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     4681 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     4741 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     4801 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     4861 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga
     4921 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     4981 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     5041 ggccggagaa tctgcgtgca atccatcttg ttcaatcata ctcttccttt ttcaatatta
     5101 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5161 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcggacg
     5221 gatcgcttgc ctgtaactta cacgcgcctc gtcctgagaa agcaacctga cctacaggaa
     5281 agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt aaaatttgta
     5341 tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata agaaattcgc
     5401 ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca tcttttcgta
     5461 aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca aacctctggc
     5521 gaagaattgt tagagctctc actgcccact ttccagtctg gaaacctgtc ttgctagctg
     5581 catgagtgaa tcagccaatg cccggggaga ggcagtttgt gtattgggtg ccagggtggt
     5641 ttttctcttc accagtgaga ctggcaacag ctgattgccc ttcaccgcct ggccctgaga
     5701 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     5761 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     5821 atctgcacca actctcagcc cagactcagt aatggctctc attgcaccca gtgccatctg
     5881 atcattggca accagcattg cagtgggaac aatgccctca ttcagcattt gcatggtttg
     5941 ttgaaaccca gacatggcac tccagtcccc ttctctttca gctattggct gaatttgatt
     6001 cctagtgaga tatttatgcc agccggccag tctcagcctt gctgagacag aactgagtgg
     6061 gcccgcaagc agtgcaattt gctggtgtcc caatgcaacc agatgctcca cacccagtct
     6121 tgtaccatct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     6181 aaagagtgct ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     6241 tggatagtta atgatcagcc cactgactct ttgtgccaga agattgtgca cagcagcttt
     6301 acaggcttca actccacttc tttctaccat tgacaccacc acagaggctc ccagttgatc
     6361 agctctagat ttaatggctg ccacaatttg agatggtgca tgcagggcca gactggaggt
     6421 ggcaactcca atcagcaagc tctgtttgcc tgccagttgt tgtgccactc tgttgggaat
     6481 gtaattcagc tctgccatgg ctgcttccac tttttccctg gttttggcag aaacatggct
     6541 ggcctggttc accactctgg aaacagtctg ataagagaca ccggcatact ctgcgacatc
     6601 atacaatgtt actggtttca ttttaataac tagtgcgaaa aaaccccgcc gaagcggggt
     6661 tttttgcgtt aaagccggtg acggtagtcg ctgggagtcc ggtcaaattg acgagcgaag
     6721 acgcgagaga aggtttgctg cgacacatag cctaagtcca tggcaatatc gaaaatcgga
     6781 cgttctgttg tccgcaattc aacggcagcc aacaacaaac ggcgttgacg gatatagtct
     6841 cctaatgttt gatgtgtgac ggtacgaaac atgcgctgta aataccactt cgagtaacca
     6901 cttttcttcg ctacaacatc aatattaaga ggctgatcaa tatgctcatc gatccaagca
     6961 ataaggtcct ggatgatttt ttggtggctc attgaaccac ctccgccggg tttagtgttg
     7021 ccatctataa cagatctaag ttcatcccaa cttgctgagt ctcctatgaa atctccaccg
     7081 ttgccgttcc agaaactcat caggaggtta ttatcattac cagtatatgt cggttgatta
     7141 aaaccaacta tatgttggtt ccaataagga ttctgtggct gtggaagcgg cggctcgtgg
     7201 gacatggaat ctactgtacg gtggctcgtg tggtcggaat ctaagctgct ggtcttagaa
     7261 caagtgtcgg ttaaagtgat aaccggtttt cggtttggtg gtaagatggt tgggtttctt
     7321 tgagatgtga ctcgtttgaa gattctgcaa agtgtccata cctctgcttg ttgagctgga
     7381 gagtcggttt tcgtggtgga ggggaggcgg aattcatgca tcatccaatc ggttttggtg
     7441 cctttaccgg ctgaaccaag atagtaaacc agagatttct tgagaccaac acagtcaaga
     7501 ttggagtaaa ccggtttatc aataccagtg gctttccaga aacctgaacc ggtcactcgg
     7561 tttggtcgaa cgctattcct gtatttccta cctctcatgc agaagaagta ccactccttt
     7621 tctccgacgc tgctcactct tggaagatcc caaggatcgt acttatagat atcgatctgt
     7681 ttgataagtt cgagtttgat ggttttgttc tctacttttc ttcgaagata gtatcctaaa
     7741 agctcttcat ccgtcggatg aaacctgaac ccaggaagtg gtgcttcgtt ttcttcttca
     7801 tgatccttac ctaagttacc ttcgccacta tgtcgacaga tcctttctcc tctttagatc
     7861 ttttgaattc ccaaaaaaac gggtatggag aaacagtaga gagttgcgat aaaaagcgtc
     7921 aggtagaatc cgctaatctt atggataaaa atgctatggc atagcaaagt gtgacgccgt
     7981 gcaaataatc aatgtggact tttctgccgt gattatagac acttttgtta cgcgtttttg
     8041 tcatggcttt ggtcccgctt tgttacagaa tgcttttaat aagcggggtt accggtttgg
     8101 ttagcgagaa gagccagtaa aagacgcagt gacggcaatg tctgatgcaa tatggacaat
     8161 tggtttcttc tctgaatggc gggagtatga aaagttcaac atttccgtgt cgcccttatt
     8221 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8281 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8341 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8401 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8461 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8521 acggatggc
//
