LOCUS       sequence-423976-        2714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP sequence for C-terminus tagging of human 
            cadherin 5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Allen Institute
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-167:CDH5-mEGFP
FEATURES             Location/Qualifiers
     source          1..2714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(692..703)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(1001..1003,1004..1717)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcatcccagc tttacttatg tatatagtag tttgttcagt aactcaaagc tagaaggcct
       61 gagcctaatt ttagaagctc tctgcacttc aaggtctacc cctccttagg gatccaagac
      121 cctcacatag ccacacccct cagagattcc cacaggctga gcatccgcac ctgcccctgg
      181 gtgtggggga acggcagcgg tgggagtgtc gggcgaggtg gggatgagta tgcgggggag
      241 cggcagggac ggggtgtggg gtgtaggggg atggcgggga tgggggtatg ggggaacggc
      301 ggggatgggg ttgcagggga aggggggcca aaaggatggg gttgcagggg aagggggggc
      361 caaagggatg ggcatgctgg gagggcgtgg gggtggggtt gcagggggca gtggggatgg
      421 gggcatgggg ggccgcccag gcccccagcc ttgtctgact ctgctgctcg gctccctggc
      481 tgcagtgatc accctgctca tcttcctgcg gcggcggctc cggaagcagg cccgcgcgca
      541 cggcaagagc gtgccggaga tccacgagca gctggtcacc tacgacgagg agggcggcgg
      601 cgagatggac accaccagct acgatgtgtc ggtgctcaac tcggtgcgcc gcggcggggc
      661 caagcccccg cggcccgcgc tggacgcccg gccttccctc tatgcgcagg tgcagaagcc
      721 accgaggcac gcgcctgggg cacacggagg gcccggggag atggcagcca tgatcgaggt
      781 gaagaaggac gaggcggacc acgacggcga cggccccccc tacgacacgc tgcacatcta
      841 cggctacgag ggctccgagt ccatagccga gtccctcagc tccctgggca ccgactcatc
      901 cgactctgac gtggattacg acttccttaa cgactgggga cccaggttta agatgctggc
      961 tgagctgtac ggctcggacc cccgggagga gctgctgtat gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagtaggcg gccgaggtca ctctgggcct
     1741 ggggacccaa accccctgca gcccaggcca gtcagacgcc aggcaccaca gcctccaaaa
     1801 atggcagtga ctccccagcc cagcacccct tcctcgtggg tcccagagac ctcatcagcc
     1861 ttgggatagc aaactccagg ttcctgaaat atccaggaat atatgtcagt gatgactatt
     1921 ctcaaatgct ggcaaatcca ggctggtgtt ctgtctgggc tcagacatcc acataaccct
     1981 gtcacccaca gaccgccgtc taactcaaag acttcctctg gctccccaag gctgcaaagc
     2041 aaaacagact gtgtttaact gctgcagggt ctttttctag ggtccctgaa cgccctggta
     2101 aggctggtga ggtcctggtg cctatctgcc tggaggcaaa ggcctggaca gcttgacttg
     2161 tggggcagga ttctctgcag cccattccca agggagactg accatcatgc cctctctcgg
     2221 gagccctagc cctgctccaa ctccatactc cactccaagt gccccaccac tccccaaccc
     2281 ctctccaggc ctgtcaagag ggaggaaggg gccccatggc agctcctgac cttgggtcct
     2341 gaagtgacct cactggcctg ccatgccagt aactgtgctg tactgagcac tgaaccacat
     2401 tcagggaaat ggcttattaa actttgaagc aactgtgaat tcattctgga ggggcagtgg
     2461 agatcaggag tgacagatca cagggtgagg gccacctcca cacccacccc ctctggagaa
     2521 ggcctggaag agctgagacc ttgctttgag actcctcagc acccctccag ttttgcctga
     2581 gaaggggcag atgttcccgg agcagaagac gtctcccctt ctctgcctca cctggtcgcc
     2641 aatccatgct ctctttcttt tctctgtcta ctccttatcc cttggtttag aggaacccaa
     2701 gatgtggcct ttag
//
