LOCUS       sequence-423977-        7567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter system to measure EJC-enhanced NMD activity. No
            intron in 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Kolakada D, Campbell AE, Galvis LB, Li Z, Lore M, Jagannathan S
  TITLE     A system of reporters for comparative investigation of 
            EJC-independent and EJC-enhanced nonsense-mediated mRNA decay.
  JOURNAL   Nucleic Acids Res. 2024 Feb 20:gkae121. doi: 10.1093/nar/gkae121.
  PUBMED    38375914
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Feb 20:gkae121. doi: 10.1093/nar/gkae121."
COMMENT     Sequence Label: ARTi
FEATURES             Location/Qualifiers
     source          1..7567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    1049..1170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1183..1216)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1430..1449
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1448..1503
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1549..1568
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1645..2358,2359..2361,2362..2364))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFRTAAGITHGMDELYK"
     regulatory      2361..2370
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2397..2421)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2987..3007
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3070..3079
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3079..3108
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3109..3807
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="GEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTMKI
                     KVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVL
                     TATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQMA
                     LKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAVAR
                     YCDLPSKLGHS"
     CDS             3862..3927
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3928..3981
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3982..4023
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4825..4851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4992..5113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5209..5320
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5309..5326
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5462..6061)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5545..5565)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6042..6061
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    complement(6066..6099)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    6311..6359
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6432..6536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6537..6605,6606..7397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6755..6774)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tgggtaccta agatacattg atgagtttgg acaaaccaca
     1081 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1141 gtaaccatta taagctgcaa taaacaagtt gacggtatcg taataacttc gtatagcata
     1201 cattatacga agttatatgg atccatatgc tagctcgacg ctcaggtcga gggatcttca
     1261 taagagaaga gggacagcta tgactgggag taaggaatgg acagcagggg gctgtttcat
     1321 atactgatga cctctttata gccacctttg ttcatggcag ccagcatatg gcatatgttg
     1381 ccaaactcta aaccaaatac tcattctgat gttttaaatg atttgccctc ccatatgtcc
     1441 ttccgagtga gagacacaaa aaattccaac acactattgc aatgaaaata aatttccttt
     1501 attagccaga agtcagatgc tcaaggggct tcatgatgtc cccataattt ttggcagagg
     1561 gaaaaagatc tcagtggtat ttgtgagcca gggcattggc cacaccagcc accaccttct
     1621 gataggcagc ctgcacctga gtgtttactt gtacagctcg tccatgccgt gagtgatccc
     1681 ggcggcggtg cggaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
     1741 cagcttggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     1801 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
     1861 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agatgttgtg
     1921 gctgttgaag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
     1981 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
     2041 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
     2101 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
     2161 gccgtggctc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
     2221 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
     2281 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
     2341 cagctcctcg cccttgctca ccatggtggc gaccacgatc ctcatcctgt ctctgcgatc
     2401 tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg ccacctcgac
     2461 atactcagta gttattaata gtaatcaatt acggggtcat tagttcatag cgaggctgca
     2521 gtctaccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagataccg
     3061 gtcgccaccg ccaccatgga acaaaagctg atttctgaag aagacttggg tgaggatagc
     3121 gtgctgatca ccgagaacat gcacatgaaa ctgtacatgg agggcaccgt gaacgaccac
     3181 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaag
     3241 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
     3301 atgtacggca gcaaaacctt tatcaaccac acccagggca tccccgactt ctttaagcag
     3361 tccttccctg agggcttcac atgggagagg atcaccacat acgaagacgg gggcgtgctg
     3421 accgctaccc aggacaccag cctccagaac ggctgcctca tctacaacgt caagatcaac
     3481 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     3541 agcaccgaga tgctgtaccc cgctgacagc ggcctgagag gccatagcca gatggccctg
     3601 aagctcgtgg gcgggggcta cctgcactgc tccctcaaga ccacatacag atccaagaaa
     3661 cccgctaaga acctcaagat gcccggcttc tacttcgtgg acaggagact ggaaagaatc
     3721 aaggaggccg acaaagagac ctacgtcgag cagcacgaga tggctgtggc tcgctactgc
     3781 gacctgccta gcaaactggg gcacagcctc gagggtggtt ctggtggttc tgcctaaggt
     3841 ggttctggtg gttctgaatt cgactacaag gaccacgacg gtgactacaa ggaccacgac
     3901 atcgactaca aggacgacga cgacaaggag ggcagaggaa gtctgctaac atgcggtgac
     3961 gtcgaggaga atcctggccc aggtaagcct atccctaacc ctctcctcgg tctcgattct
     4021 acgagcgggg gcgaggagct gttcgccggc atcgtgcccg tgctgatcga gctggacggc
     4081 gacgtgcacg gccacaagtt cagcgtgcgc ggcgagggcg agggcgacgc cgactacggc
     4141 aagctggaga tcaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctg
     4201 gtgaccaccc tctgctacgg catccagtgc ttcgcccgct accccgagca catgaagatg
     4261 aacgacttct tcaagagcgc catgcccgag ggctacatcc aggagcgcac catccagttc
     4321 caggacgacg gcaagtacaa gacccgcggc gaggtgagtg ctacatggtg agccccaaag
     4381 ctggtgtggg aggagccacc tggctgatgg gcagcccctt cataccctca cgtattcccc
     4441 caggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg caaggacttc
     4501 aaggaggacg gcaacatcct gggccacaag ctggagtaca gcttcaacag ccacaacgtg
     4561 tacatccgcc ccgacaaggc caacaacggc ctggaggcta acttcaagac ccgccacaac
     4621 atcgagggcg gcggcgtgca gctggccgac cactaccaga ccaacgtgcc cctgggcgac
     4681 ggccccgtgc tgatccccat caaccactac ctgagcactc agaccaagat cagcaaggac
     4741 cgcaacgagg cccgcgacca catggtgctc ctggagtcct tcagcgcctg ctgccacacc
     4801 cacggcatgg acgagctgta caggtaccca tacgatgttc cagattacgc tatgcattaa
     4861 atagatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     4921 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     4981 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5041 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5101 tcaatgtatc ttaacgcgaa actagtgcta ttgtcttccc aatcctcccc cttgctgtcc
     5161 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
     5221 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
     5281 gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgagct
     5341 ctagagtcgg tgggcctcgg gggcgggtgc ggggtcggcg gggccgcccc gggtggcttc
     5401 ggtcggagcc atggggtcgt gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc
     5461 gtcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt
     5521 cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct
     5581 ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc
     5641 tgcccagacc cttgccctgg tggtcgggcg acacgccgac ggtggccagg aaccacgcgg
     5701 gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg
     5761 aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc
     5821 tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga
     5881 tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc
     5941 ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc
     6001 gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca
     6061 tggtgataac ttcgtatagg atactttata cgaagttatt tattctcgaa tgggggaccc
     6121 cgtccctaac ccacggggcc agtggctatg gcagggcctg ccgccccgac gttggctgcg
     6181 agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg
     6241 tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc
     6301 ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt
     6361 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tattaagctt ggcacttttc
     6421 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6481 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6541 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6601 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6661 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6721 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6781 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6841 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6901 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6961 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7021 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7081 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7141 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7201 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7261 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7321 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7381 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7441 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7501 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7561 gatcttc
//
