LOCUS       sequence-424051-       12629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of wild-type adult isoform of human SCN8A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Vanoye CG, Abramova TV, DeKeyser JM, Ghabra NF, Oudin MJ, Burge CB, 
            Helbig I, Thompson CH, George AL Jr
  TITLE     Molecular and cellular context influences SCN8A variant function.
  JOURNAL   JCI Insight. 2024 May 21;9(12):e177530. doi: 
            10.1172/jci.insight.177530.
  PUBMED    38771640
REFERENCE   2  (bases 1 to 12629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2024-05-21"; volume: "9(12):e177530. doi"; pages: 
            " 10.1172/jci.insight.177530"
COMMENT     Sequence Label: pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet
FEATURES             Location/Qualifiers
     source          1..12629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          4371..4503
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    join(6191..6763,6764..6766,6767..6775)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6357..6374)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6584..6603
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6776..7471
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6909..6927)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7177..7196
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7525..7554
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7570..7587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7610..7627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7616..7840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7886..8314
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7973..7992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8183..8204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8323..8343)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8328..8657
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8508..8643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8570..8589
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8705..8752
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8771..9145
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9275..9396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9312..9331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9366..9385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9445..9461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9445..9461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9458..9480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9469..9485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9493..9499,9500..9517,9518..9523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9538..9559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9676..9693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9847..10435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9927..9946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10606..11397,11398..11466))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11229..11248
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11467..11571)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11646..11665)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11837..12216
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12217..12420
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12371..12391
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    12422..12440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12443..12461
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     12471..12495
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggcagcgc ggctgcttgc accaccaggc cctgatagtt tcaagccttt cacccctgag
       61 tcactggcaa acattgagag gcgcattgct gagagcaagc tcaagaaacc accaaaggcc
      121 gatggcagtc atcgggagga cgatgaggac agcaagccca agccaaacag cgacctggaa
      181 gcagggaaga gtttgccttt catctacggg gacatccccc aaggcctggt tgcagttccc
      241 ctggaggact ttgacccata ctatttgacg cagaaaacct ttgtagtatt aaacagaggg
      301 aaaactctct tcagatttag tgccacgcct gccttgtaca ttttaagtcc ttttaacctg
      361 ataagaagaa tagctattaa aattttgata cattcagtat ttagcatgat cattatgtgc
      421 actattttga ccaactgtgt attcatgact tttagtaacc ctcctgactg gtcgaagaat
      481 gtggagtaca cgttcacagg gatttataca tttgaatcac tagtgaaaat cattgcaaga
      541 ggtttctgca tagatggctt taccttttta cgggacccat ggaactggtt agatttcagt
      601 gtcatcatga tggcatatgt gacagagttt gtggacctgg gcaatgtctc agcgctgaga
      661 acattcaggg ttctccgagc tttgaaaact atctctgtaa ttccaggcct gaagacaatt
      721 gtgggtgccc tgattcagtc tgtgaagaaa ctgtcagatg tgatgatcct gacagtgttc
      781 tgcctgagtg tttttgcctt gatcggactg cagctgttca tggggaacct tcgaaacaag
      841 tgtgttgtgt ggcccataaa cttcaacgag agctatcttg aaaatggcac caaaggcttt
      901 gattgggaag agtatatcaa caataaaaca aatttctaca cagttcctgg catgctggaa
      961 cctttactct gtgggaacag ttctgatgct gggcaatgcc cagagggata ccagtgtatg
     1021 aaagcaggaa ggaaccccaa ctatggttac acaagttttg acacttttag ctgggccttc
     1081 ttggcattat ttcgccttat gacccaggac tattgggaaa acttgtatca attgacttta
     1141 cgagcagccg ggaaaacata catgatcttc ttcgtcttgg tcatctttgt gggttctttc
     1201 tatctggtga acttgatctt ggctgtggtg gccatggctt atgaagaaca gaatcaggca
     1261 acactggagg aggcagaaca aaaagaggct gaatttaaag caatgttgga gcaacttaag
     1321 aagcaacagg aagaggcaca ggctgctgcg atggccactt cagcaggaac tgtctcagaa
     1381 gatgccatag aggaagaagg tgaagaagga gggggctccc ctcggagctc ttctgaaatc
     1441 tctaaactca gctcaaagag tgcaaaggaa agacgtaaca ggagaaagaa gaggaagcaa
     1501 aaggaactct ctgaaggaga ggagaaaggg gatcccgaga aggtgtttaa gtcagagtca
     1561 gaagatggca tgagaaggaa ggcctttcgg ctgccagaca acagaatagg gaggaaattt
     1621 tccatcatga atcagtcact gctcagcatc ccaggctcgc ccttcctctc ccgccacaac
     1681 agcaagagca gcatcttcag tttcagggga cctgggcggt tccgagaccc gggctccgag
     1741 aatgagttcg cggatgacga gcacagcacg gtggaggaga gcgagggccg ccgggactcc
     1801 ctcttcatcc ccatccgggc ccgcgagcgc cggagcagct acagcggcta cagcggctac
     1861 agccagggca gccgctcctc gcgcatcttc cccagcctgc ggcgcagcgt gaagcgcaac
     1921 agcacggtgg actgcaacgg cgtggtgtcc ctcatcggcg gccccggctc ccacatcggc
     1981 gggcgtctcc tgccagaggc tacaactgag gtggaaatta agaagaaagg ccctggatct
     2041 cttttagttt ccatggacca attagcctcc tacgggcgga aggacagaat caacagtata
     2101 atgagtgttg ttacaaatac actagtagaa gaactggaag agtctcagag aaagtgcccg
     2161 ccatgctggt ataaatttgc caacactttc ctcatctggg agtgccaccc ctactggata
     2221 aaactgaaag agattgtgaa cttgatagtt atggaccctt ttgtggattt agccatcacc
     2281 atctgcatcg tcctgaatac actgtttatg gcaatggagc accatcctat gacaccacaa
     2341 tttgaacatg tcttggctgt aggaaatctg gtaagtatga aattcaggga tacggcatat
     2401 ttgccaaata gtggaaatgt gaagtactga caaaactttt ccctttttcc ctctcatagg
     2461 ttttcactgg aattttcaca gcggaaatgt tcctgaagct catagccatg gatccctact
     2521 attatttcca agaaggttgg aacatttttg acggatttat tgtctccctc agtttaatgg
     2581 aactgagtct agcagacgtg gaggggcttt cagtgctgcg atctttccga ttgctccgag
     2641 tcttcaaatt ggccaaatcc tggcccaccc tgaacatgct aatcaagatt attggaaatt
     2701 cagtgggtgc cctgggcaac ctgacactgg tgctggccat tattgtcttc atctttgccg
     2761 tggtggggat gcaactcttt ggaaaaagct acaaagagtg tgtctgcaag atcaaccagg
     2821 actgtgaact ccctcgctgg catatgcatg actttttcca ttccttcctc attgtctttc
     2881 gagtgttgtg cggggagtgg attgagacca tgtgggactg catggaagtg gcaggccagg
     2941 ccatgtgcct cattgtcttt atgatggtca tggtgattgg caacttggtg gtgctgaacc
     3001 tgtttctggc cttgctcctg agctccttca gtgcagacaa cctggctgcc acagatgacg
     3061 atggggaaat gaacaacctc cagatctcag tgatccgtat caagaagggt gtggcctgga
     3121 ccaaactaaa ggtgcacgcc ttcatgcagg cccactttaa gcagcgtgag gctgatgagg
     3181 tgaagcctct ggatgagttg tatgaaaaga aggccaactg tatcgccaat cacaccggtg
     3241 cagacatcca ccggaatggt gacttccaga agaatggcaa tggcacaacc agcggcattg
     3301 gcagcagcgt ggagaagtac atcattgatg aggaccacat gtccttcatc aacaacccca
     3361 acttgactgt acgggtaccc attgctgtgg gcgagtctga ctttgagaac ctcaacacag
     3421 aggatgttag cagcgagtcg gatcctgaag gcagcaaaga taaactagat gacaccagct
     3481 cctctgaagg aagcaccatt gatatcaaac cagaagtaga agaggtccct gtggaacagc
     3541 ctgaggaata cttggatcca gatgcctgct tcacagaagg ttgtgtccag cggttcaagt
     3601 gctgccaggt caacatcgag gaagggctag gcaagtcttg gtggatcctg cggaaaacct
     3661 gcttcctcat cgtggagcac aactggtttg agaccttcat catcttcatg attctgctga
     3721 gcagtggcgc cctggccttc gaggacatct acattgagca gagaaagacc atccgcacca
     3781 tcctggaata tgctgacaaa gtcttcacct atatcttcat cctggagatg ttgctcaagt
     3841 ggacagccta tggcttcgtc aagttcttca ccaatgcctg gtgttggctg gacttcctca
     3901 ttgtggctgt ctctttagtc agccttatag ctaatgccct gggctactcg gaactaggtg
     3961 ccataaagtc ccttaggacc ctaagagctt tgagaccctt aagagcctta tcacgatttg
     4021 aagggatgag ggtggtggtg aatgccttgg tgggcgccat cccctccatc atgaatgtgc
     4081 tgctggtgtg tctcatcttc tggctgattt tcagcatcat gggagttaac ttgtttgcgg
     4141 gaaagtacca ctactgcttt aatgagactt ctgaaatccg atttgaaatt gaagatgtca
     4201 acaataagac cgaatgtgaa aagcttatgg aggggaacaa tacagagatc agatggaaga
     4261 acgtgaagat caactttgac aatgttgggg caggatacct ggcccttctt caagtagcaa
     4321 ccttcaaagg ctggatggac atcatgtatg cagctgtaga ttcccggaag gtaagtatca
     4381 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     4441 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     4501 cagcctgatg agcagcctaa gtatgaggac aatatctaca tgtacatcta ttttgtcatc
     4561 ttcatcatct tcggctcctt cttcaccctg aacctgttca ttggtgtcat cattgataac
     4621 ttcaatcaac aaaagaaaaa gttcggaggt caggacatct tcatgaccga agaacagaag
     4681 aagtactaca atgccatgaa aaagctgggc tcaaagaagc cacagaaacc tattccccgc
     4741 cccttgaaca aaatccaagg aatcgtcttt gattttgtca ctcagcaagc ctttgacatt
     4801 gttatcatga tgctcatctg ccttaacatg gtgacaatga tggtggagac agacactcaa
     4861 agcaagcaga tggagaacat cctctactgg attaacctgg tgtttgttat cttcttcacc
     4921 tgtgagtgtg tgctcaaaat gtttgcgttg aggcactact acttcaccat tggctggaac
     4981 atcttcgact tcgtggtagt catcctctcc attgtgggaa tgttcctggc agatataatt
     5041 gagaaatact ttgtttcccc aaccctattc cgagtcatcc gattggcccg tattgggcgc
     5101 atcttgcgtc tgatcaaagg cgccaaaggg attcgtaccc tgctctttgc cttaatgatg
     5161 tccttgcctg ccctgttcaa catcggcctt ctgctcttcc tggtcatgtt catcttctcc
     5221 atttttggga tgtccaattt tgcatatgtg aagcacgagg ctggtatcga tgacatgttc
     5281 aactttgaga catttggcaa cagcatgatc tgcctgtttc aaatcacaac ctcagctggt
     5341 tgggatggcc tgctgctgcc catcctaaac cgcccccctg actgcagcct agataaggaa
     5401 cacccaggga gtggctttaa gggagattgt gggaacccct cagtgggcat cttcttcttt
     5461 gtaagctaca tcatcatctc tttcctaatt gtcgtgaaca tgtacattgc catcatcctg
     5521 gagaacttca gtgtagccac agaggaaagt gcagaccctc tgagtgagga tgactttgag
     5581 accttctatg agatctggga gaagttcgac cccgatgcca cccagttcat tgagtactgt
     5641 aagctggcag actttgcaga tgccttggag catcctctcc gagtgcccaa gcccaatacc
     5701 attgagctca tcgctatgga tctgccaatg gtgagcgggg atcgcatcca ctgcttggac
     5761 atcctttttg ccttcaccaa gcgggtcctg ggagatagcg gggagttgga catcctgcgg
     5821 cagcagatgg aagagcggtt cgtggcatcc aatccttcca aagtgtctta cgagccaatc
     5881 acaaccacac tgcgtcgcaa gcaggaggag gtatctgcag tggtcctgca gcgtgcctac
     5941 cggggacatt tggcaaggcg gggcttcatc tgcaaaaaga caacttctaa taagctggag
     6001 aatggaggca cacaccggga gaaaaaagag agcaccccat ctacagcctc cctcccgtcc
     6061 tatgacagtg taactaaacc tgaaaaggag aaacagcagc gggcagagga aggaagaagg
     6121 gaaagagcca aaagacaaaa agaggtcaga gaatccaagt gttgactcga gcgatcggcc
     6181 cttggatccg cccctctccc tccccccccc taacgttact ggccgaagcc gcttggaata
     6241 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     6301 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     6361 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     6421 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     6481 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     6541 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6601 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6661 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     6721 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     6781 gagcaagggc gaggcagtga tcaaggagtt catgcggttc aaggtgcaca tggagggctc
     6841 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     6901 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct
     6961 gtcccctcag ttcatgtacg gctccagggc cttcaccaag caccccgccg acatccccga
     7021 ctactataag cagtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     7081 cggcggcgcc gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa
     7141 ggtgaagctc cgcggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat
     7201 gggctgggaa gcgtccaccg agcggttgta ccccgaggac ggcgtgctga agggcgacat
     7261 taagatggcc ctgcgcctga aggacggagg ccgctacctg gcggacttca agaccaccta
     7321 caaggccaag aagcccgtgc agatgcccgg cgcctacaac gtcgaccgca agttggacat
     7381 cacctcccac aacgaggact acaccgtggt ggaacagtac gaacgctccg agggccgcca
     7441 ctccaccggc ggcatggacg agctgtacaa gtaacctgca ggaattcaag ggcgaattcg
     7501 tttgctctag agggcccgcg gttcgaacaa aaactcatct cagaagagga tctgaatatg
     7561 cataccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     7621 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7681 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7741 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7801 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7861 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7921 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7981 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8041 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8101 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8161 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8221 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8281 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     8341 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8401 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8461 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8521 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8581 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8641 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     8701 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     8761 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     8821 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     8881 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     8941 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     9001 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     9061 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     9121 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     9181 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     9241 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     9301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     9421 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10261 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    10321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    11041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    11101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    11161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    11221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    11281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    11341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11581 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
    11641 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    11701 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    11761 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
    11821 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
    11881 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
    11941 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
    12001 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
    12061 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
    12121 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
    12181 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
    12241 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
    12301 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
    12361 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
    12421 ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta
    12481 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
    12541 cgggaccgat ccagcctccg gactctagcg tttaaactta agcttggtac cgagctcgga
    12601 tccactagtc cagtgtggtg gaattcgcc
//
