LOCUS       sequence-424065-       12031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D 
            S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter
            and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12031)
  TITLE     Dual promoter expression vectors including protrudin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-ZFYVE27 S14D S25D S32D
            T261D-WPRE-UbC...
FEATURES             Location/Qualifiers
     source          1..12031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3783..3803)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4201..4212)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5251..5270
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5444..5453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5450..5452,5453..5455,5456..6169)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5495..5516)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5756..5775)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6103..6124
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6264..6497
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6522..6540)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6523..6540)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6830..6849)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7063..7082)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7182..7204
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7242..7260)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7328..7432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7433..7501,7502..8293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7651..7670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8464..9052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8953..8972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9206..9223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9293..9313)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9298..9627
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9478..9613
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10761..10826
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10956..10976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11401..11535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11438..11457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11492..11511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11703..12031,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatccaccgg tcgccaccat gcagacatca gaacgtgagg ggagtgggcc ggagctggat
     2461 cccagcgtga tgcccgaggc tcccctggag gatccacctt ttcctaccaa ggatccagcg
     2521 tttgaccttt tcaacttggt tctctcctac aagaggctgg agatctacct ggaacccttg
     2581 aaggatgcag gtgatggtgt tcgatacttg ctcaggtggc agatgccttt gtgttccttg
     2641 ctgacctgcc tgggcctcaa cgtcttgttc ctcactttga atgagggtgc atggtactca
     2701 gtaggtgccc tgatgatttc agtgcccgcc ctgctgggct accttcagga ggtttgccgg
     2761 gcacggctgc ctgattccga gctgatgcgg aggaagtatc atagcgtgag gcaggaggac
     2821 ctgcagagag gtcgcctgtc tcgtcccgag gccgtggctg aggtgaagag cttcttgatc
     2881 cagctggagg ccttcctgag ccgcctgtgc tgcacatgtg aagccgccta ccgcgtgctg
     2941 cactgggaga accccgtcgt gtcctcacag ttctatgggg ctcttctggg cacagtctgc
     3001 atgctgtatt tgctgccact ctgctgggtt ctcacccttt taaacagcac gctctttctg
     3061 gggaatgtgg agttcttccg agttgtgtct gagtacaggg catctctgca gcagaggatg
     3121 aacccaaagc aggaagagca tgcctttgag agtcctccac caccagatgt tggggggaag
     3181 gatggtctga tggacagcga tcctgccctc acacccacgg agagtctctc ttcccaggac
     3241 ctcacaccgg gcagcgtgga ggaggctgag gaggctgagc cagatgaaga gtttaaagat
     3301 gcgattgagg agacccactt ggtggtgctg gaggatgatg agggcgcccc gtgcccagca
     3361 gaggatgagc tggccctgca ggacaacggg ttcctgagca agaatgaggt gctgcgcagc
     3421 aaggtgtctc ggctcacgga gcggctccgc aagcgctacc ccaccaacaa cttcgggaac
     3481 tgcacgggct gctcggccac cttctcagtg ctgaagaaga ggcggagctg cagtaattgt
     3541 ggaaacagct tctgctctcg atgctgctcc ttcaaggtgc ccaagtcctc catgggggcc
     3601 acagcccctg aagcccagag ggagactgtg tttgtgtgtg cctcgtgtaa ccagaccttg
     3661 agcaagtgaa gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     3721 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3781 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3841 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3901 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3961 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4021 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4081 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4141 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4201 tcggccctca atccagcgga ccttccttcc cgcgggcgcc cccctcctca cggcgagcgc
     4261 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     4321 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     4381 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     4441 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     4501 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     4561 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     4621 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     4681 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     4741 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     4801 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     4861 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     4921 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     4981 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     5041 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     5101 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     5161 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     5221 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     5281 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     5341 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     5401 cttttttgtt agacgaagct tggtaccgag ctcggatcaa ttcgccacca tggtgagcaa
     5461 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5521 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5581 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5641 cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     5701 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5761 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5821 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5881 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
     5941 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     6001 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     6061 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6121 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac tcgagggaat
     6181 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6241 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6301 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6361 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6421 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6481 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     6541 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     6601 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     6661 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     6721 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     6781 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     6841 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     6901 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     6961 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     7021 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     7081 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7141 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     7201 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     7261 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     7321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     9301 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9361 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9421 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9481 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9541 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9601 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     9661 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     9721 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     9781 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     9841 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     9901 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     9961 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
    10021 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
    10081 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
    10141 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
    10201 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
    10261 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
    10321 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
    10381 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
    10441 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
    10501 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
    10561 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
    10621 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
    10681 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
    10741 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
    10801 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
    10861 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
    10921 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
    10981 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
    11041 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
    11101 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
    11161 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
    11221 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
    11281 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
    11341 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    11401 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11461 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11521 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    11581 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    11641 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    11701 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
    11761 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
    11821 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
    11881 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
    11941 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
    12001 agagctgcat ccggagtact tcaagaactg c
//
