LOCUS       sequence-424067-        1783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lip2 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Jiang W, Wang S, Avila P, Jorgensen TS, Yang Z, Borodina I
  TITLE     Combinatorial iterative method for metabolic engineering of Yarrowia
            lipolytica: application for betanin biosynthesis.
  JOURNAL   Metab Eng. 2024 Sep 9:S1096-7176(24)00110-1. doi: 
            10.1016/j.ymben.2024.09.003.
  PUBMED    39260817
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2024 Sep 9:S1096-7176(24)00110-1. doi: 10.1016/j.ymben.2024.09.003."
COMMENT     Sequence Label: pCfB13090
FEATURES             Location/Qualifiers
     source          1..1783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      235..823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     724..743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1072..1106)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1177..1783,1..53))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1751..1770
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
       61 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
      121 ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg
      181 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      241 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      301 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      361 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      421 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      481 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      541 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      601 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      661 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      721 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      781 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      841 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      901 ccctgattct gtggataacc gtagtcggtc tcatctagtg tctgtggtat ctaagctatt
      961 tatcactctt tacaacttct acctcaacta tctactttaa taaatgaata tcgtttattc
     1021 tctatgatta ctgtatatgc gttcctctaa gacagagttg agaccagacc aataaaaaac
     1081 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1141 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1201 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1261 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1321 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1381 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     1441 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1501 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1561 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     1621 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1681 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     1741 ggtacattga gcaactgact gaaatgcctc aaaatgttct tta
//
