LOCUS       sequence-424069-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Padi SKR, Vos MR, Godek RJ, Fuller JR, Kruse T, Hein JB, Nilsson J, 
            Kelker MS, Page R, Peti W
  TITLE     Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19.
  JOURNAL   Nature. 2024 Jan;625(7993):195-203. doi: 10.1038/s41586-023-06870-3.
            Epub 2023 Dec 20.
  PUBMED    38123684
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06870-3"; journalName: "Nature"; date: 
            "2024-01"; volume: "625"; issue: "7993"; pages: "195-203"
COMMENT     Sequence Label: pcDNA3.4_StrepII_TEV_PP2Ac
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..30
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atggtatgga gccacccgca gttcgaaaaa gaaaacctgt attttcaggg catggacgag
       61 aaggtgttca ccaaggagct ggaccagtgg atcgagcagc tgaacgagtg caagcagctg
      121 tccgagtccc aggtcaagag cctctgcgag aaggctaaag aaatcctgac aaaagaatcc
      181 aacgtgcaag aggttcgatg tccagttact gtctgtggag atgtgcatgg gcaatttcat
      241 gatctcatgg aactgtttag aattggtggc aaatcaccag atacaaatta cttgtttatg
      301 ggagattatg ttgacagagg atattattca gttgaaacag ttacactgct tgtagctctt
      361 aaggttcgtt accgtgaacg catcaccatt cttcgaggga atcatgagag cagacagatc
      421 acacaagttt atggtttcta tgatgaatgt ttaagaaaat atggaaatgc aaatgtttgg
      481 aaatatttta cagatctttt tgactatctt cctctcactg ccttggtgga tgggcagatc
      541 ttctgtctac atggtggtct ctcgccatct atagatacac tggatcatat cagagcactt
      601 gatcgcctac aagaagttcc ccatgagggt ccaatgtgtg acttgctgtg gtcagatcca
      661 gatgaccgtg gtggttgggg tatatctcct cgaggagctg gttacacctt tgggcaagat
      721 atttctgaga catttaatca tgccaatggc ctcacgttgg tgtctagagc tcaccagcta
      781 gtgatggagg gatataactg gtgccatgac cggaatgtag taacgatttt cagtgctcca
      841 aactattgtt atcgttgtgg taaccaagct gcaatcatgg aacttgacga tactctaaaa
      901 tactctttct tgcagtttga cccagcacct cgtagaggcg agccacatgt tactcgtcgt
      961 accccagact acttcctgta a
//
