LOCUS       sequence-424076-        3792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from 
            R. toruloides for a P1 position (overhangs R/D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Bonturi N, Pinheiro MJ, de Oliveira PM, Rusadze E, Eichinger T, 
            Liudziute G, De Biaggi JS, Brauer A, Remm M, Miranda EA, 
            Ledesma-Amaro R, Lahtvee PJ
  TITLE     Development of a dedicated Golden Gate Assembly Platform (RtGGA) for
            Rhodotorula toruloides.
  JOURNAL   Metab Eng Commun. 2022 May 23;15:e00200. doi: 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec.
  PUBMED    35662893
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2022-05-23"; volume: "15:e00200. doi"; pages: " 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec"
COMMENT     Sequence Label: (R)pGPD1(D)_P1(SBE109)
FEATURES             Location/Qualifiers
     source          1..3792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(1624..1630,1631..1648,1649..1654))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1669..1690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1810..1827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1981..2569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2061..2080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2740..3531,3532..3600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3363..3382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3601..3705)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3773..3791
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tatgcggtct cacatttgga gttcgacgtt ctcctcgctc cgcaagcatt
      421 ggaatgaacc ttgctctcta ggtccctcct ccgtgacctc gtttcgtcct ttagacggca
      481 cgatggaagg aagaaatctc tgcggacaag caaatctgct ggctcgcctt gtaggtcgcc
      541 taccggagca agccttgtgc cgccgggatg ccaacgtcgt tttttgacgt ttgcaagacg
      601 tagaggacgc ttcggacgac gaaacaagct gtgaggacat ggaagtcgtg ggaggaacgg
      661 cgcagagcgg cgccgcggga gcataaggca agcgagatag tccagaaatc gcggcgccaa
      721 gtacagtaat ttattggagc aggcaccaga agcgggcagc agtatgcgca ggcttggggt
      781 cgacgagaga cgactccctc atactcggtt acctcgagca atacaatcaa tcgaagctgc
      841 gcgaatctcg gcttgtaagg gtcggaaagg aacctcggag atggccacgt cacatcacca
      901 acttatcgat ctcagccgac gtcgcagaga gggcgagcga agcggtgaag gagggaaaca
      961 atccctcgag agcatgatcc gtctgaatct gcagcgcagg aagccgtcac acgcccgcct
     1021 cgagcgcagg tcgggtccag ccgggggacg aaacgcgcga gggctgattt cgtgagcgaa
     1081 ggaagccgca tcgacaagtt cgcgtccctt tgcactcttt cccatcaccc gctctcgctc
     1141 tacccgctca gaacaacacc agatcagtca caacacggag acctgctcta tctttctaga
     1201 agatctccta caatattctc agctgccatg gaaaatcgat gttcttcttt tattctctca
     1261 agattttcag gctgtatatt aaaacttata ttaagaacta tgctaaccac ctcatcagga
     1321 accgttgtag gtggcgtggg ttttcttggc aatcgactct catgaaaact acgagctaaa
     1381 tattcaatat gttcctcttg accaacttta ttctgcattt tttttgaacg aggtttagag
     1441 caagcttcag gaaactgaga caggaatttt attaaaaatt taaattttga agaaagttca
     1501 gggttaatag catccatttt ttgctttgca agttcctcag cattcttaac aaaagacgtc
     1561 tcttttgaca tgtttaaagt ttaaacctcc tgtgtgaaat tattatccgc tcataattcc
     1621 acacattata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1681 actcacatta attgcgttgc gctcactgcc aattgctttc cagtcgggaa acctgtcgtg
     1741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3721 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3781 tatcacgagg cc
//
