LOCUS       sequence-424078-       11527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression vector 6xHisTag-TEV at N terminus to express S 
            cerevisiae topoisomerase II (1–1177).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Jeong J, Lee JH, Carcamo CC, Parker MW, Berger JM
  TITLE     DNA-Stimulated Liquid-Liquid phase separation by eukaryotic 
            topoisomerase ii modulates catalytic function.
  JOURNAL   Elife. 2022 Nov 7;11:e81786. doi: 10.7554/eLife.81786.
  PUBMED    36342377
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-11-7"; volume: "11:e81786. doi"; pages: " 
            10.7554/eLife.81786"
COMMENT     Sequence Label: 12URA-B-ScTop2∆CTD
FEATURES             Location/Qualifiers
     source          1..11527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..665
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    217..334
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     629..652
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             693..710
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             720..740
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(4365..5168)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5169..5384)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      5976..7318
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6939..6986)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(7676..7694)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7762..7866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7867..7935,7936..8727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8085..8104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8898..9486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9387..9406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9640..9657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9672..9812
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9738..9757)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9898..9920
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9914..10105)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tttcaaaaat tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca
       61 tggcattacc accatataca tatccatata catatccata tctaatctta cttatatgtt
      121 gtggaaatgt aaagagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca
      181 gtaatacgct taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt
      241 gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct
      301 gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc
      361 ttttatggtt atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa
      421 cgaatcaaat taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg
      481 gtaattaatc agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact
      541 gcataaccac tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat
      601 gtaataaaag tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa
      661 aaaaccccgg atcagtaacc atgggcagca gccatcatca tcatcatcac agcagcggcg
      721 aaaacctgta cttccaggga tcctcaactg aaccggtaag cgcctctgat aaatatcaga
      781 aaatttctca actggaacat atcttaaaaa gaccagacac ttatatcggt tctgttgaaa
      841 ctcaagagca gctgcaatgg atatacgatg aagagaccga ttgcatgatt gaaaaaaatg
      901 tcacaattgt accagggttg ttcaaaatct ttgatgaaat cttagtcaat gcggcagata
      961 ataaagttcg tgatccatcg atgaaacgaa tcgatgtaaa catacatgct gaggaacata
     1021 ctatagaagt gaaaaatgat ggaaaaggta ttcccataga gattcataac aaggagaata
     1081 tttatattcc tgaaatgata tttggtcatt tgttgacatc atccaattat gatgatgatg
     1141 agaagaaagt cactggtggt agaaacggtt atggtgctaa gctttgtaat atattttcca
     1201 ctgaattcat attagaaact gcagatctaa atgttggcca gaaatatgtt caaaaatggg
     1261 aaaataacat gagcatttgc caccccccaa aaataacatc ttacaagaag ggtccatcat
     1321 atacaaaggt gacatttaag ccggatttaa ccagattcgg aatgaaagag ctagataatg
     1381 atatcttagg agtgatgcga agaagagttt atgatatcaa tggttctgtt cgtgacatta
     1441 atgtctatct gaatggcaag tccttaaaga taagaaattt caaaaattat gttgaactct
     1501 acttgaaatc actcgaaaaa aaaagacaac tagataacgg tgaggacggt gccgctaagt
     1561 ctgatatccc gactattctt tatgagagaa taaacaacag atgggaagtt gcttttgcgg
     1621 tttctgatat ctcttttcaa caaatttctt ttgtgaattc cattgcaact accatgggtg
     1681 gtacccatgt caattacata acagaccaaa ttgtaaaaaa aatttcagaa attttgaaga
     1741 aaaagaagaa aaaaagtgtg aagtcttttc agattaaaaa taatatgttc attttcatta
     1801 attgtttgat tgagaatcct gcatttacct cacaaacaaa agagcaactg acaacaagag
     1861 tcaaagattt tgggtcccgt tgtgagattc ctcttgaata tattaataag attatgaaaa
     1921 ctgatttggc tacaagaatg tttgaaattg ccgacgcaaa tgaagaaaat gcgctaaaga
     1981 agtctgatgg tacaaggaaa agcagaatta ctaattaccc taaactggaa gatgccaaca
     2041 aagccggtac aaaagaaggc tataaatgta ctttagttct gacagaaggg gattccgcct
     2101 tgtcattagc tgttgcaggt ttagctgttg ttggtagaga ttattatggt tgttatccac
     2161 ttcgtggtaa aatgctgaat gttagagaag ctagtgctga tcagatacta aaaaacgcgg
     2221 aaattcaagc cattaaaaaa attatggggt tacaacatcg caagaaatat gaagatacaa
     2281 aatctttaag atatgggcat cttatgatca tgaccgatca agatcatgat ggttcgcata
     2341 ttaaaggttt aattataaac tttttagaaa gctcatttcc tggtcttttg gatatccaag
     2401 gtttcttact tgaattcata actccgatca tcaaagtttc catcactaaa ccaacaaaaa
     2461 acactattgc attctacaat atgccggact atgaaaaatg gagagaggaa gaatcgcaca
     2521 aatttacttg gaagcagaag tattataaag gattagggac ttctctagca caagaagtcc
     2581 gagaatattt ttcgaacttg gacagacatt tgaaaatatt ccattctttg cagggtaatg
     2641 ataaagatta cattgattta gctttctcca agaaaaaggc agatgaccgt aaagaatggc
     2701 tgagacaata cgaacctggt actgttttag accctacttt aaaagagatt ccaattagcg
     2761 acttcattaa taaggaatta atcctttttt ctttggccga taatatacgg tcgattccca
     2821 atgttttaga tggatttaaa cctggccaaa gaaaagttct ttatggttgt ttcaaaaaaa
     2881 atttaaagtc ggaactgaaa gtagctcaac ttgcaccata cgtgagcgaa tgtacggcat
     2941 atcaccatgg tgagcagtca ttggcacaaa ctattattgg gctagcccaa aactttgttg
     3001 ggtccaacaa tatttacttg ctattaccta acggtgcttt cggtacaaga gccactggtg
     3061 gtaaagatgc agcggcagcg agatatatct acacagaatt gaacaaatta actcgtaaga
     3121 tatttcaccc tgctgatgat ccattataca aatatataca agaagatgag aaaacagtgg
     3181 agccagagtg gtatttacca attcttccta tgattcttgt taacggtgct gagggtattg
     3241 gcactggctg gagtacttac attcctccat tcaacccatt ggaaattata aagaatataa
     3301 gacatttaat gaacgacgag gagcttgagc aaatgcatcc gtggtttagg ggatggaccg
     3361 gtactattga agaaattgag cctctgcgtt acagaatgta cggtaggatt gaacaaattg
     3421 gagataacgt cttagaaata actgagttgc cagccagaac ttggacatcg actataaagg
     3481 agtacttact tttaggttta agcggtaacg ataaaataaa accctggatc aaagatatgg
     3541 aggagcagca cgatgataac atcaaattca taatcacgct atcacctgag gaaatggcta
     3601 aaacaaggaa aataggtttt tatgaaagat ttaaactaat ttcgcctata agtttgatga
     3661 atatggtcgc atttgatcct cacgggaaaa tcaagaagta caattccgtg aatgaaatat
     3721 taagcgaatt ttactacgtc agactagaat actatcaaaa aagaaaagac catatgagcg
     3781 aaaggttaca gtgggaggta gagaaatact ctttccaagt aaaatttatt aaaatgatta
     3841 ttgaaaagga gttaacagtc accaataagc ctaggaacgc tattatccaa gaacttgaga
     3901 atttagggtt ccccagattt aataaggaag gtaaaccata ttatggaagt cctaacgatg
     3961 agatagctga acaaattaac gacgtaaaag gcgcaacttc tgatgaagaa gatgaagaaa
     4021 gttcacacga agatactgaa aatgttataa atggtcctga agaactatat ggcacatatg
     4081 aatatttatt aggaatgaga atatggtcat tgaccaagga aagatatcaa aagctgttga
     4141 aacaaaaaca agaaaaggag acagagttgg aaaacttgtt aaaactttcc gcgaaagata
     4201 tatggaacac tgacttgaag gcttttgagg tgggatatca agaatttttg caacgagatg
     4261 cagaagctcg ctaataactc gaggccgggt aataactgat ataattaaat tgaagctcta
     4321 atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg
     4381 catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca
     4441 tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc
     4501 acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca
     4561 ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca
     4621 ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat
     4681 tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt
     4741 tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca
     4801 ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc
     4861 aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac
     4921 ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata
     4981 tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat
     5041 tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata
     5101 ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct
     5161 ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat
     5221 actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg
     5281 tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga
     5341 aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagctt atcgatgata
     5401 agctgtcaaa catgagaatt ccacggacta tagactatac tagtatactc cgtctactgt
     5461 acgatacact tccgctcagg tccttgtcct ttaacgaggc cttaccactc ttttgttact
     5521 ctattgatcc agctcagcaa aggcagtgtg atctaagatt ctatcttcgc gatgtagtaa
     5581 aactagctag accgagaaag agactagaaa tgcaaaaggc acttctacaa tggctgccat
     5641 cattattatc cgatgtgacg ctgcagcttc tcaatgatat tcgaatacgc tttgaggaga
     5701 tacagcctaa tatccgacaa actgttttac agatttacga tcgtacttgt tacccatcat
     5761 tgaattttga acatccgaac ctgggagttt tccctgaaac agatagtata tttgaacctg
     5821 tataataata tatagtctag cgctttacgg aagacaatgt atgtatttcg gttcctggag
     5881 aaactattgc atctattgca taggtaatct tgcacgtcgc atccccggtt cattttctgc
     5941 gtttccatct tgcacttcaa tagcatatct ttgttaacga agcatctgtg cttcattttg
     6001 tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     6061 ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat
     6121 ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     6181 catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac
     6241 ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta
     6301 gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact
     6361 gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa
     6421 agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca
     6481 agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca
     6541 gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt
     6601 ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc
     6661 tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa
     6721 aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat
     6781 atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag
     6841 cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag
     6901 tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagag
     6961 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     7021 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     7081 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     7141 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     7201 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     7261 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat
     7321 atgcatagta ccgagaaact agtgcgaagt agtgatcagg tattgctgtt atctgatgag
     7381 tatacgttgt cctggccacg gcagaagcac gcttatcgct ccaatttccc acaacattag
     7441 tcaactccgt taggcccttc attgaaagaa atgaggtcat caaatgtctt ccaatgtgag
     7501 attttgggcc attttttata gcaaagattg aataaggcgc atttttcttc aaagctttat
     7561 tgtacgatct gactaagtta tcttttaata attggtattc ctgtttattg cttgaagaat
     7621 tgccggtcct atttactcgt tttaggactg gttcagaatt cttgaagacg aaagggcctc
     7681 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7741 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7801 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7861 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7921 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7981 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8041 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8101 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8161 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8221 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8281 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8341 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8401 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8461 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8521 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8581 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8641 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8701 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8761 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8821 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8881 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8941 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9001 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     9061 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9661 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     9721 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     9781 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     9841 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     9901 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     9961 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
    10021 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
    10081 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
    10141 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
    10201 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
    10261 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
    10321 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
    10381 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
    10441 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
    10501 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
    10561 tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga
    10621 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
    10681 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc
    10741 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
    10801 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
    10861 aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga
    10921 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
    10981 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
    11041 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
    11101 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
    11161 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
    11221 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
    11281 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
    11341 gttgaaggct ctcaagggca tcggtcgacg gtcgaccaat tcgacaggtt atcagcaaca
    11401 acacagtcat atccattctc aattagctct accacagtgt gtgaaccaat gtatccagca
    11461 ccacctgtaa ccaaaacaat tttagaagta ctttcacttt gtaactgagc tgtcatttat
    11521 attgaat
//
