LOCUS       sequence-424087-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral overexpression of human TK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Flickinger KM, Wilson KM, Rossiter NJ, Hunger AL, Vishwasrao PV, Lee
            TD, Mellado Fritz CA, Richards RM, Hall MD, Cantor JR
  TITLE     Conditional lethality profiling reveals anticancer mechanisms of 
            action and drug-nutrient interactions.
  JOURNAL   Sci Adv. 2024 Oct 4;10(40):eadq3591. doi: 10.1126/sciadv.adq3591. 
            Epub 2024 Oct 4.
  PUBMED    39365851
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-10-4"; volume: "10(40):eadq3591. doi"; pages: " 
            10.1126/sciadv.adq3591. Epub 2024 Oct 4"
COMMENT     Sequence Label: pLJC2-TK2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             817..882
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgttgctgt ggcccttgcg agggtgggca gctagagccc tcagatgttt cggtccgggt
       61 tcacgaggga gtccggcatc aggacccgga cctcggcgcg tacaacgacg cgcttggccg
      121 ccagataagg agcaagagaa ggaaaagaaa tcagtaatct gtgtggaggg taacatagct
      181 agtgggaaga caacctgtct cgagttcttc agcaatgcca cggacgtaga agtcctgacg
      241 gaacccgtat caaaatggag gaacgttcgg ggtcacaacc ccctgggtct catgtaccat
      301 gatgcaagca gatggggtct taccctgcag acttatgtgc agcttactat gttggatcga
      361 catacccgcc cgcaggtttc tagtgtacgg ttgatggaga ggagcataca cagtgcccgc
      421 tatatctttg tcgaaaacct ctacaggtcc ggcaaaatgc ccgaggtcga ttacgttgta
      481 ctcagtgagt ggtttgattg gatactgaga aatatggacg tgtccgttga cctcatcgtg
      541 tacctccgga ctaatcccga aacctgctat caacgactca agaagcgctg tagggaagaa
      601 gagaaggtga tcccactgga gtatcttgaa gccattcacc atttgcacga ggaatggttg
      661 atcaagggtt ctctctttcc tatggcagca ccggtgttgg tgatagaagc cgaccatcac
      721 atggaaagaa tgttggagtt gtttgaacag aacagagaca ggatacttac accagaaaat
      781 cgcaaacact gcccaggagg cagcgcggcc gcaggcgact acaaagacca tgacggtgat
      841 tataaagatc atgacattga ttacaaggat gacgatgaca agtaataa
//
