LOCUS       sequence-424088-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral overexpression of human DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Flickinger KM, Wilson KM, Rossiter NJ, Hunger AL, Vishwasrao PV, Lee
            TD, Mellado Fritz CA, Richards RM, Hall MD, Cantor JR
  TITLE     Conditional lethality profiling reveals anticancer mechanisms of 
            action and drug-nutrient interactions.
  JOURNAL   Sci Adv. 2024 Oct 4;10(40):eadq3591. doi: 10.1126/sciadv.adq3591. 
            Epub 2024 Oct 4.
  PUBMED    39365851
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-10-4"; volume: "10(40):eadq3591. doi"; pages: " 
            10.1126/sciadv.adq3591. Epub 2024 Oct 4"
COMMENT     Sequence Label: pLJC2-DHFR-3xFLAG
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             583..648
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggtaggat ccttgaattg tatcgtcgcg gtatctcaaa atatggggat aggaaaaaac
       61 ggcgacttgc cgtggccgcc gcttagaaac gagttcaggt actttcagcg aatgactacc
      121 acatctagcg tcgaggggaa gcagaacctt gtgattatgg gcaaaaaaac atggttttca
      181 ataccggaga agaaccggcc cctcaaaggc cgcattaatt tggtattgag tagagaactg
      241 aaagagccac cccagggtgc tcattttctg agtaggtccc ttgacgatgc tctgaagttg
      301 acagaacagc ctgagttggc gaacaaggtg gatatggtat ggatcgtcgg aggctcatct
      361 gtatacaaag aagccatgaa ccatcccggc caccttaaac tgtttgttac caggataatg
      421 caagacttcg aatctgatac attttttccc gagatcgact tggaaaagta caaactgttg
      481 cctgaatacc ctggtgttct ctcagatgtt caggaggaga aggggataaa atataagttt
      541 gaggtttacg agaagaacga tggaggcagc gcggccgcag gcgactacaa agaccatgac
      601 ggtgattata aagatcatga cattgattac aaggatgacg atgacaagta ataa
//
