LOCUS       sequence-424089-        2898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral overexpression of human MTHFD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Flickinger KM, Wilson KM, Rossiter NJ, Hunger AL, Vishwasrao PV, Lee
            TD, Mellado Fritz CA, Richards RM, Hall MD, Cantor JR
  TITLE     Conditional lethality profiling reveals anticancer mechanisms of 
            action and drug-nutrient interactions.
  JOURNAL   Sci Adv. 2024 Oct 4;10(40):eadq3591. doi: 10.1126/sciadv.adq3591. 
            Epub 2024 Oct 4.
  PUBMED    39365851
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-10-4"; volume: "10(40):eadq3591. doi"; pages: " 
            10.1126/sciadv.adq3591. Epub 2024 Oct 4"
COMMENT     Sequence Label: pLJC2-MTHFD1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..2898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2827..2892
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggcgccag cagaaatcct gaacgggaag gagatctccg cgcaaataag ggcgagactg
       61 aaaaatcaag tcactcagtt gaaggagcaa gtacctggtt tcacaccacg cctggcaata
      121 ttacaggttg gcaacagaga tgattccaat ctttatataa atgtgaagct gaaggctgct
      181 gaagagattg ggatcaaagc cactcacatt aagttaccaa gaacaaccac agaatctgag
      241 gtgatgaagt acattacatc tttgaatgaa gactctactg tacatgggtt cttagtgcag
      301 ctacctttag attcagagaa ttccattaac actgaagaag tgatcaatgc tattgcaccc
      361 gagaaggatg tggatggatt gactagcatc aatgctggga aacttgctag aggtgacctc
      421 aatgactgtt tcattccttg tacgcctaag ggatgcttgg aactcatcaa agagacaggg
      481 gtgccgattg ccggaaggca tgctgtggtg gttgggcgca gtaaaatagt tggggccccg
      541 atgcatgact tgcttctgtg gaacaatgcc acagtgacca cctgccactc caagactgcc
      601 catctggatg aggaggtaaa taaaggtgac atcctggtgg ttgcaactgg tcagcctgaa
      661 atggttaaag gggagtggat caaacctggg gcaatagtca tcgactgtgg aatcaattat
      721 gtcccagatg ataaaaaacc aaatgggaga aaagttgtgg gtgatgtggc atacgacgag
      781 gccaaagaga gggcgagctt catcactcct gttcctggcg gcgtagggcc catgacagtt
      841 gcaatgctca tgcagagcac agtagagagt gccaagcgtt tcctggagaa atttaagcca
      901 ggaaagtgga tgattcagta taacaacctt aacctcaaga cacctgttcc aagtgacatt
      961 gatatatcac gatcttgtaa accgaagccc attggtaagc tggctcgaga aattggtctg
     1021 ctgtctgaag aggtagaatt atatggtgaa acaaaggcca aagttctgct gtcagcacta
     1081 gaacgcctga agcaccggcc tgatgggaaa tacgtggtgg tgactggaat aactccaaca
     1141 cccctgggag aagggaaaag cacaactaca atcgggctag tgcaagccct tggtgcccat
     1201 ctctaccaga atgtctttgc gtgtgtgcga cagccttctc agggccccac ctttggaata
     1261 aaaggtggcg ctgcaggagg cggctactcc caggtcattc ctatggaaga gtttaatctc
     1321 cacctcacag gtgacatcca tgccatcact gcagctaata acctcgttgc tgcggccatt
     1381 gatgctcgga tatttcatga actgacccag acagacaagg ctctctttaa tcgtttggtg
     1441 ccatcagtaa atggagtgag aaggttctct gacatccaaa tccgaaggtt aaagagacta
     1501 ggcattgaaa agactgaccc taccacactg acagatgaag agataaacag atttgcaaga
     1561 ttggacattg atccagaaac cataacttgg caaagagtgt tggataccaa tgatagattc
     1621 ctgaggaaga tcacgattgg acaggctcca acggagaagg gtcacacacg gacggcccag
     1681 tttgatatct ctgtggccag tgaaattatg gctgtcctgg ctctcaccac ttctctagaa
     1741 gacatgagag agagactggg caaaatggtg gtggcatcca gtaagaaagg agagcccgtc
     1801 agtgccgaag atctgggggt gagtggtgca ctgacagtgc ttatgaagga cgcaatcaag
     1861 cccaatctca tgcagacact ggagggcact ccagtgtttg tccatgctgg cccgtttgcc
     1921 aacatcgcac atggcaattc ctccatcatt gcagaccgga tcgcactcaa gcttgttggc
     1981 ccagaagggt ttgtagtgac ggaagcagga tttggagcag acattggaat ggaaaagttt
     2041 tttaacatca aatgccggta ttccggcctc tgcccccacg tggtggtgct tgttgccact
     2101 gtcagggctc tcaagatgca cgggggcggc cccacggtca ctgctggact gcctcttccc
     2161 aaggcttaca tacaggagaa cctggagctg gttgaaaaag gcttcagtaa cttgaagaaa
     2221 caaattgaaa atgccagaat gtttggaatt ccagtagtag tggccgtgaa tgcattcaag
     2281 acggatacag agtctgagct ggacctcatc agccgccttt ccagagaaca tggggctttt
     2341 gatgccgtga agtgcactca ctgggcagaa gggggcaagg gtgccttagc cctggctcag
     2401 gccgtccaga gagcagcaca agcacccagc agcttccagc tcctttatga cctcaagctc
     2461 ccagttgagg ataaaatcag gatcattgca cagaagatct atggagcaga tgacattgaa
     2521 ttacttcccg aagctcaaca caaagctgaa gtctacacga agcagggctt tgggaatctc
     2581 cccatctgca tggctaaaac acacttgtct ttgtctcaca acccagagca aaaaggtgtc
     2641 cctacaggct tcattctgcc cattcgcgac atccgcgcca gcgttggggc tggttttctg
     2701 taccccttag taggaacgat gagcacaatg cctggactcc ccacccggcc ctgtttttat
     2761 gatattgatt tggaccctga aacagaacag gtgaatggat tattcggagg cagcgcggcc
     2821 gcaggcgact acaaagacca tgacggtgat tataaagatc atgacattga ttacaaggat
     2881 gacgatgaca agtaataa
//
